Alterations in B cell development, CDR-H3 repertoire and dsDNA-binding antibody production among C57BL/6 ΔD−iD mice congenic for the lupus susceptibility loci sle1, sle2 or sle3

Tyrosine and glycine constitute 40% of complementarity determining region 3 of the immunoglobulin heavy chain (CDR-H3), the center of the classic antigen-binding site. To assess the role of DH RF1-encoded tyrosine and glycine in regulating CDR-H3 content and potentially influencing B cell function, we created mice limited to a single DH encoding asparagine, histidine, and arginines in RF1. Tyrosine and glycine content in CDR-H3 was halved. Bone marrow and spleen mature B cell and peritoneal cavity B-1 cell numbers were also halved, whereas marginal zone B cell numbers increased. Serum immunoglobulin G subclass levels and antibody titers to T-dependent and T-independent antigens all declined. Thus, violation of the conserved preference for tyrosine and glycine in DH RF1 alters CDR-H3 content and impairs B cell development and antibody production.

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Preferential Use of DHReading Frame 2 Alters B Cell Development and Antigen-Specific Antibody Production

The Journal of Immunology ◽

10.4049/jimmunol.181.12.8409 ◽

2008 ◽

Vol 181 (12) ◽

pp. 8409-8415 ◽

Cited By ~ 21

Author(s):

Robert L. Schelonka ◽

Michael Zemlin ◽

Ryoki Kobayashi ◽

Gregory C. Ippolito ◽

Yingxin Zhuang ◽

...

Keyword(s):

B Cell ◽

Antibody Production ◽

Specific Antibody ◽

Cell Development ◽

B Cell Development ◽

Specific Antibody Production

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Variation in genes implicated in B‐cell development and antibody production affects susceptibility to pemphigus

Immunology ◽

10.1111/imm.13259 ◽

2020 ◽

Vol 162 (1) ◽

pp. 58-67

Author(s):

Verónica Calonga‐Solís ◽

Leonardo M. Amorim ◽

Ticiana D. J. Farias ◽

Maria Luiza Petzl‐Erler ◽

Danielle Malheiros ◽

...

Keyword(s):

B Cell ◽

Antibody Production ◽

Cell Development ◽

B Cell Development

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B lymphocytes: how they develop and function

Blood ◽

10.1182/blood-2008-02-078071 ◽

2008 ◽

Vol 112 (5) ◽

pp. 1570-1580 ◽

Cited By ~ 533

Author(s):

Tucker W. LeBien ◽

Thomas F. Tedder

Keyword(s):

B Cell ◽

B Lymphocytes ◽

Antibody Production ◽

Cell Development ◽

B Cell Development ◽

Molecular Events ◽

Congenital Immunodeficiency ◽

1960S And 1970S ◽

The 1960S ◽

And Function

Abstract The discovery that lymphocyte subpopulations participate in distinct components of the immune response focused attention onto the origins and function of lymphocytes more than 40 years ago. Studies in the 1960s and 1970s demonstrated that B and T lymphocytes were responsible primarily for the basic functions of antibody production and cell-mediated immune responses, respectively. The decades that followed have witnessed a continuum of unfolding complexities in B-cell development, subsets, and function that could not have been predicted. Some of the landmark discoveries that led to our current understanding of B lymphocytes as the source of protective innate and adaptive antibodies are highlighted in this essay. The phenotypic and functional diversity of B lymphocytes, their regulatory roles independent of antibody production, and the molecular events that make this lineage unique are also considered. Finally, perturbations in B-cell development that give rise to certain types of congenital immunodeficiency, leukemia/lymphoma, and autoimmune disease are discussed in the context of normal B-cell development and selection. Despite the significant advances that have been made at the cellular and molecular levels, there is much more to learn, and cross-disciplinary studies in hematology and immunology will continue to pave the way for new discoveries.

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The Roles of Bursal Nonapeptide (BP9) on AIV Vaccine Immune Response in Chick Immunization and on Avian Immature B Cell

Protein and Peptide Letters ◽

10.2174/0929866526666190730101455 ◽

2019 ◽

Vol 26 (12) ◽

pp. 940-948

Author(s):

Yang Zheng ◽

Man M. Zong ◽

Bo Y. Chen ◽

Xiao H. Zhou ◽

Zi N. Liu ◽

...

Keyword(s):

Gene Expression ◽

B Cell ◽

Antibody Production ◽

Cell Development ◽

B Cell Development ◽

Mrna Levels ◽

Biological Processes ◽

Gene Expressions ◽

Antibody Titers ◽

Dt40 Cells

Background: Bursa of Fabricius plays the vital functions on B cell development and antibody production in poultry. The bursal-derived peptide plays the essential roles on avian immature B cell development. Objectives: Here we explored the functions of the recently reported bursal nonapeptide (BP9) on the antibody production and the molecular basis of BP9 on avian immature B cell. Methods: Chicken were twice immunized with Avian Influenza Virus (AIV) inactivated vaccine plus with BP9 at three dosages, respectively. On two weeks after the second immunization, sera samples were collected from all experimental groups to measure AIV-specific Agglutination Inhibition (HI) antibody titers. Also, on 7th day after the second immunization, spleen lymphocytes were isolated from the immunized chicken to detect the lymphocyte viabilities. DT40 cells were treated with BP9 from 0.02 to 2 μg/mL for 4 and 20h to detect sIgM mRNA levels, and total RNAs from BP9-treated DT40 cells were collected to investigate the gene expression profiles of DT40 cells, and to analyze the enriched pathways and functional biological processes. Finally, nine gene expressions were validated with quantitative PCR (qPCR). Results: Our investigation proved the strong regulatory roles of BP9 on AIV-specific HI antibody titers and lymphocyte viabilities. BP9 promoted sIgM mRNA levels in DT40 cells, and upregulated 598 gene expressions and downregulated 395 gene expressions in DT40 cells with 0.2μg/mL BP9 treatment. Moreover, our findings verified the significantly enriched six pathways and various the biological functional processes of BP9 on avian immature B cell. Also, we found eight signaling pathways in the enriched biological processes of BP9-treated DT40 cells, and the expressions of nine selected genes with qPCR were identical to that of microarray data. Conclusion: BP9 promoted the antibody production in the 21-old-day chicken immunization, and stimulated the sIgM expression in DT40 cells. Furthermore, we analyzed the gene expression profile and immune-related biological processes of DT40 cells treated with BP9, which provided some new insights into the mechanism on immature B cell development, and provided important references for adjuvant development on vaccine improvement and clinical application.

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