Modulating effect of a new ester, 28-O-phosphatidylbetulin (DAPB), obtained from hen egg yolk lecithin and betulin on lymphocyte subsets and humoral immune response in mice

2019 ◽  
Vol 41 (2) ◽  
pp. 231-241
Author(s):  
Magdalena Lis ◽  
Barbara Barycza ◽  
Angelika Sysak ◽  
Aleksandra Pawlak ◽  
Agnieszka Suszko-Pawłowska ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Lymphocyte Subsets ◽  
Egg Yolk ◽  
Humoral Immune ◽  
Hen Egg Yolk ◽  
Hen Egg

scholarly journals Yolkin Isolated from Hen Egg Yolk as a Natural Immunoregulator, Activating Innate Immune Response in BMDM Macrophages

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
W. Kazana ◽  
M. Mitkiewicz ◽  
M. Ochnik ◽  
M. Sochocka ◽  
A. Zambrowicz ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Innate Immune Response ◽  
Egg Yolk ◽  
The Body ◽  
Innate Immune ◽  
Type I ◽  
Dependent Manner ◽  
Hen Egg Yolk ◽  
Hen Egg

One of the goals of biomedical sciences is to search and identify natural compounds that are safe, have no side effects, and possess immunostimulatory activity. It has been proven that medicines of natural origin can be effective agents, supporting the therapy of many diseases, not only in the weakened immune system of the body but also in the prevention of many diseases in healthy people. It has been shown that yolkin, a polypeptide complex isolated from hen egg yolk as a fraction accompanying immunoglobulin Y (IgY), possesses potential biological activity. However, the mechanism of its action has not been explained. The objective of this investigation was to examine the molecular mechanisms of innate immune response, activated in response to yolkin, in murine bone marrow-derived macrophages (BMDM). It was shown that yolkin induced phosphorylation of extracellular signal-kinases (ERK1/2) and c-Jun N-terminal kinase (JNK) and upregulated expression and production of type I interferons, TNF-α (tumor necrosis factor α), and nitric oxide (NO), in BMDM cells. Using pharmacological inhibitors of ERK 1/2 and JNK kinases, we revealed that the JNK signaling cascade is required for yolkin-induced inducible NOS expression and upregulation of NO production in mouse macrophages. Using the TLR4-deficient BMDM cell line, we established that yolkin can activate macrophages in a TLR4-dependent manner. It was also shown that NO, TNF-α, and type I IFNs (α/β) produced by BMDM cells in response to yolkin triggered antiviral activity. These data indicate that yolkin affects the regulation of the immune system and antiviral response; therefore, it can be used as an effective immunostimulator of the innate immunity or as a supplement of the conventional therapy of immunodeficiency.

Hawthorn (Crataegus monogyna) Phenolic Extract Modulates Lymphocyte Subsets and Humoral Immune Response in Mice

Planta Medica ◽  
2019 ◽  
Vol 86 (02) ◽  
pp. 160-168 ◽  
Author(s):  
Magdalena Lis ◽  
Marianna Szczypka ◽  
Agnieszka Suszko-Pawłowska ◽  
Anna Sokół-Łętowska ◽  
Alicja Kucharska ◽  
...  
Keyword(s):  
Immune Response ◽  
T Lymphocytes ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Humoral Immune Response ◽  
Lymphocyte Subsets ◽  
Mesenteric Lymph Nodes ◽  
Crataegus Monogyna ◽  
Humoral Immune ◽  
Phenolic Extract

AbstractThis study investigated the effect of hawthorn (Crataegus monogyna) phenolic extract on lymphocyte subsets in the lymphoid organs in nonimmunized mice and on humoral immune response in sheep red blood cell-immunized mice. Hawthorn phenolic extract (50, 100, 200 mg/kg) was administered orally five or ten times. Sheep red blood cells were injected 24 h after administration of the last extract dose. The lymphocyte subsets were assessed 24 and 72 h after the last dose. Humoral immune response was determined 4 and 7 days after immunization. Five doses of the extract decreased the percentage of CD4−CD8− and CD4+ thymocytes but elevated the percentage of CD4+CD8+ and CD8+ thymic cells. The extract increased the total number, percentage, and absolute count of T and B splenocytes. When administered five times, it lowered the percentage of T lymphocytes, but boosted the population of B lymphocytes of mesenteric lymph nodes (after 24 h). However, a rise in the population of T lymphocytes was observed 72 h after five and ten doses. The extract administered ten times elevated the number of plaque-forming cells and total anti-sheep red blood cell hemagglutinin titer but reduced the 2-ME-resistant antibody titer (day 7). At the same time, five doses of the extract increased antibody titers. Considering its impact on lymphocyte subsets and humoral immune response, hawthorn extract may be used as an immunomodulator.

The effects of florfenicol on lymphocyte subsets and humoral immune response in mice

2011 ◽  
Vol 14 (2) ◽  
Author(s):  
M. Lis ◽  
M. Szczypka ◽  
A. Suszko ◽  
M. Świtała ◽  
B. Obmińska-Mrukowicz
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Lymphocyte Subsets ◽  
Humoral Immune
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