Effects of media composition on submerged culture spores of the entomopathogenic fungus,Metarhizium anisopliaevar.acridumPart 2: Effects of media osmolality on cell wall characteristics, carbohydrate concentrations, drying stability, and pathogenicity

2005 ◽  
Vol 15 (4) ◽  
pp. 393-409 ◽  
Author(s):  
Jarrod E. Leland ◽  
Donald E. Mullins ◽  
Larry J. Vaughan ◽  
Herman L. Warren
Keyword(s):  
Cell Wall ◽  
Entomopathogenic Fungus ◽  
Submerged Culture ◽  
Media Composition ◽  
Effects Of Media

Production of Exopolysaccharides by a Submerged Culture of an Entomopathogenic Fungus, Paecilomyces sp.

2007 ◽  
Vol 62 (7-8) ◽  
pp. 576-578
Author(s):  
Luis Lillo ◽  
Julio Alarcón ◽  
Gerardo Cabello ◽  
Sergio Águila ◽  
Joel B. Alderete
Keyword(s):  
Entomopathogenic Fungus ◽  
Submerged Culture

Exopolysaccharide basic was obtained from a submerged culture of a native Paecilomyces sp. strain isolated from Chilean soil

scholarly journals Secretion of Cryparin, a Fungal Hydrophobin

1999 ◽  
Vol 65 (12) ◽  
pp. 5431-5435 ◽  
Author(s):  
Patricia M. McCabe ◽  
Neal K. Van Alfen
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Submerged Culture ◽  
Secretory Pathway ◽  
Culture Medium ◽  
Secretory Vesicle ◽  
Cryphonectria Parasitica ◽  
Secreted Protein ◽  
Filamentous Ascomycete ◽  
A Cell

ABSTRACT Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are not glycosylated. This glycosylation occurred in the proprotein region, which is cleaved during maturation by a Kex2-like serine protease, leaving a mature form of cryparin that could be isolated from both the cell wall and culture medium. Pulse-chase labeling experiments showed that cryparin was secreted through the cell wall, without being bound, into the culture medium. The secreted protein then binds to the cell walls ofC. parasitica, where it remains. Binding of cryparin to the cell wall occurred in submerged culture, presumably because of the lectin-like properties unique to this hydrophobin. Thus, the binding of this hydrophobin to the cell wall is different from that of other hydrophobins which are reported to require a hydrophobic-hydrophilic interface for assembly.

scholarly journals An inner cell wall protein (cwp1) from conidia of the entomopathogenic fungus Beauveria bassiana

Microbiology ◽  
1995 ◽  
Vol 141 (5) ◽  
pp. 1075-1080 ◽  
Author(s):  
M. J. Bidochka ◽  
R. J. St Leger ◽  
L. Joshi ◽  
D. W. Roberts
Keyword(s):  
Cell Wall ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Cell Wall Protein ◽  
Fungus Beauveria Bassiana

scholarly journals Effects of Media composition on Asymbiotic Seed Culture of Vanda tessellata : An Approach to In vitro Conservation

2020 ◽  
Vol 30 (2) ◽  
pp. 285-295
Author(s):  
Sumana Aditya ◽  
Tustu Mondal ◽  
Nirmalya Banerjee
Keyword(s):  
Survival Rate ◽  
Seed Germination ◽  
Significant Variation ◽  
Basal Medium ◽  
Common Phenomenon ◽  
Media Composition ◽  
Basal Media ◽  
Effects Of Media ◽  
Vitamin Mixture

For initiation of seed germination and protocorm growth in Vanda tessellata (Roxb.) Hook. ex G. Don different media, namely Knudson’s C basal medium, modified Knudson’s C basal medium, Murashige and Skoog basal medium, Vacin and Went basal medium and Lindeman basal medium either alone or supplemented with vitamin or 0.1% peptone (w/v) were used. Vitamin mixture was consisted of nicotinic acid, pyridoxine and thiamine HCl at 1 : 1 : 10, respectively. For the initiation of germination, all the treatments exhibited promising results without showing significant variation. But the rate of survival of the germinated seedlings was remarkably low in all the basal media. Maximum survival rate of germinated seedlings was recorded in MS + 0.1% peptone. Necrosis of protocorms was a common phenomenon in all the treatments and 100% necrosis was recorded in LM basal medium and LM + vitamin. Addition of vitamin mixture and peptone in the basal media increased the rate of survival as well as differentiation of the germinated protocorms. Maximum rooted plantlets were recorded in VW + 0.1% peptone Plant Tissue Cult. & Biotech. 30(2): 285-295, 2020 (December)

Change of resistance to lysozyme and ultrasonic disintegration of the mycelium of Streptomyces griseus under the influence of chelating agents and polyvalent cations

1968 ◽  
Vol 14 (7) ◽  
pp. 769-773 ◽  
Author(s):  
F. Szeszák ◽  
G. Szabó ◽  
J. Erdel ◽  
F. Müller
Keyword(s):  
Cell Wall ◽  
Life Cycle ◽  
Submerged Culture ◽  
Chelating Agents ◽  
Ethylenediaminetetraacetic Acid ◽  
Streptomyces Griseus ◽  
Enhanced Resistance ◽  
Polyvalent Cations

Mycelia of Streptomyces griseus strain No. 52-1 from a 2-day submerged culture pretreated with ethylenediaminetetraacetic acid (EDTA) were digested with lysozyme. The sensitivity of younger vegetative hyphae to lysozyme increased while that of older reproductive hyphae remained almost unchanged.EDTA or borate pretreatment increased resistance to ultrasonic disintegration in the case of 96-h mycelia from submerged culture. EDTA was most effective at a concentration of 10−5 M.The enhanced resistance to ultrasonic disintegration can be reversed by Mg2+ and Al3+ in a concentration of 10−5 M, while Ca2+ ions are ineffective.These results suggest the existence of a polyvalent-ion-dependent layer in the cell wall of streptomycetes. Such a layer would be exterior to the mucopeptide layer. Its thickness changes during the life cycle and it is most prominent in younger vegetative hyphae.

scholarly journals Mr-AbaA Regulates Conidiation by Interacting with the Promoter Regions of Both Mr-veA and Mr-wetA in Metarhizium robertsii

2021 ◽  
Author(s):  
Hao Wu ◽  
Youmin Tong ◽  
Rong Zhou ◽  
Yulong Wang ◽  
Zhangxun Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Entomopathogenic Fungus ◽  
Submerged Culture ◽  
Comprehensive Understanding ◽  
Promoter Regions ◽  
Metarhizium Robertsii ◽  
Content Type

Metarhizium robertsii is an emerging model entomopathogenic fungus for developing biopesticides; therefore, a comprehensive understanding of its conidiation is very important for its application. In this study, we revealed that the transcription factor Mr-AbaA is involved in the control of aerial conidiation and blastospore separation in submerged culture.

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