Effect of Phenolic Antioxidants, BHA and BHT, on the Kinetics of Metabolic Processes of Benzo[a]pyrene and its Two Metabolites 3-Hydroxy- and 9-Hydroxy-Benzo[a]pyrene in Single Living RTG2 Cells

1994 ◽  
Vol 4 (1-2) ◽  
pp. 49-60
Author(s):  
A. Lopp ◽  
J. M. Salmon ◽  
P. Viallet ◽  
U. Kirso
Keyword(s):  
Phenolic Antioxidants ◽  
Metabolic Processes ◽  
Kinetics Of ◽  
Single Living

The effect of heterotrophic yield on the assessment of the correction factor for anoxic growth

1996 ◽  
Vol 34 (5-6) ◽  
pp. 67-74 ◽  
Author(s):  
D. Orhon ◽  
S. Sözen ◽  
N. Artan
Keyword(s):  
Correction Factor ◽  
Domestic Sewage ◽  
Yield Coefficient ◽  
Heterotrophic Growth ◽  
Metabolic Processes ◽  
Anoxic Conditions ◽  
Industrial Wastewaters ◽  
Systems Modelling ◽  
Kinetics Of ◽  
Very High

For single-sludge denitrification systems, modelling of anoxic reactors currently uses the kinetics of aerobic heterotrophic growth together with a correction factor for anoxic conditions. This coefficient is computed on the basis of respirometric measurements with the assumption that the heterotrophic yield remains the same under aerobic and anoxic coditions. The paper provides the conceptual proof that the yield coefficient is significantly lower for the anoxic growth on the basis of the energetics of the related metabolic processes. This is used for the interpretation of the very high values for the correction factor experimentally determined for a number of industrial wastewaters. A default value for the anoxic heterotrophic yield coefficient is calculated for domestic sewage and compatible wastewaters and proposed for similar evaluations.

scholarly journals Monitoring the Kinetics of the Cellular Uptake of a Metal Carbonyl Conjugated with a Lipidic Moiety in Living Cells Using Synchrotron Infrared Spectromicroscopy

2019 ◽  
Vol 74 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Sylvain Clède ◽  
Christophe Sandt ◽  
Paul Dumas ◽  
Clotilde Policar
Keyword(s):  
Breast Cancer ◽  
Cellular Uptake ◽  
Microfluidic Device ◽  
Metal Carbonyl ◽  
Living Cells ◽  
High Survival ◽  
High Survival Rate ◽  
Short Term ◽  
Kinetics Of ◽  
Single Living

Presented here is the exploitation of synchrotron infrared spectromicroscopy to evaluate the feasibility of monitoring the cellular uptake of rhenium-tris-carbonyl-tagged (Re(CO)3) lipophilic chains in living cells. To this aim, an in-house thermostated microfluidic device was used to limit water absorption while keeping cells alive. Indeed, cells showed a high survival rate in the microfluidic device over the course of the experiment, proving the short-term biocompatibility of the device. We recorded spectra of single, living, fully hydrated breast cancer MDA-MB231 cells and could follow the penetration of the rhenium complexes for up to 2 h. Despite the strong variations observed in the uptake kinetics between individual cells, the Re(CO)3 complex was traced inside the cells at low concentration and shown to enter them on the hour time scale by active transport.

Subcellular and multicelluar organization of calcium signaling in liver

1996 ◽  
Vol 54 ◽  
pp. 736-737
Author(s):  
A.P. Thomas ◽  
L.D. Robb-Gaspers
Keyword(s):  
Mammalian Cells ◽  
Hepatic Metabolism ◽  
Isolated Hepatocytes ◽  
Temporal Organization ◽  
Continuous Exposure ◽  
Imaging Studies ◽  
The Individual ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

Cytosolic Ca2+ ([Ca2+]i) is a ubiquitous intracellular messenger in mammalian cells, and imaging studies of single living cells loaded with fluorescent Ca2+ indicators have demonstrated that the [Ca2+]i changes induced by extracellular agonists are often organized in complex temporal and spatial patterns. A number of hormones that regulate hepatic metabolism, including vasopressin and α1adrenergic agonists, bring about their effects through a rise in [Ca2+]i mediated by the Ca2+-mobilizing second messenger IP3. Imaging studies of isolated hepatocytes have demonstrated that the [Ca+]i responses during continuous exposure to these hormones consist of a series of discrete [Ca+]i spikes, the frequency of which is determined by the dose of agonist. By contrast, the amplitude and kinetics of the individual [Ca+]i spikes are not affected by changing the agonist dose. In addition to the temporal organization in the form of [Ca2+]i oscillations, the [Ca2+]i changes are also spatially organized into regenerative [Ca2+]i waves that propagate throughout the cytoplasm and nucleoplasm of the cell from a discrete plasma membrane locus. These oscillatory [Ca2+]i waves are generated by the complex interplay of [Ca2+]i and IP3 in regulating the gating properties of the IP3- receptor Ca2+ channel, which is located in the endoplasmic reticulum Ca2+ store.

Modelling Interpretation of the Kinetics of Metabolic Processes

2000 ◽  
pp. 305-328 ◽  
Author(s):  
S. Bastianoni ◽  
C. Bonechi ◽  
A. Gastaldelli ◽  
S. Martini ◽  
C. Rossi
Keyword(s):  
Metabolic Processes ◽  
Kinetics Of

NF-κB activation in single living cells — Analysis of anti-apoptosis and kinetics of activation by IL-1β

1999 ◽  
Vol 27 (3) ◽  
pp. A94-A94
Author(s):  
Franço Carlotti ◽  
Lin Yang ◽  
Steven K. Dower ◽  
Eva E. Qwarnstrom
Keyword(s):  
Living Cells ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

scholarly journals The Rate of Proflavin Passage into Single Living Cells with Application to Permeability Studies

1960 ◽  
Vol 43 (4) ◽  
pp. 853-866 ◽  
Author(s):  
Elliott Robbins
Keyword(s):  
Tissue Culture ◽  
Physicochemical Properties ◽  
Single Cell ◽  
Uptake Rate ◽  
Living Cells ◽  
Dye Transport ◽  
Permeability Studies ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

A technique for the measurement of the uptake rate of proflavin by the single cell in tissue culture has been developed, and the kinetics of the dye transport are discussed in terms of its physicochemical properties. Some applications of the technique to the study of permeability are given.

Reaction Kinetics of Phenolic Antioxidants toward Photoinduced Pyranine Free Radicals in Biological Models

2017 ◽  
Vol 121 (26) ◽  
pp. 6331-6340 ◽  
Author(s):  
Alexis Aspée ◽  
Christian Aliaga ◽  
Luca Maretti ◽  
Daniel Zúñiga-Núñez ◽  
Jessica Godoy ◽  
...  
Keyword(s):  
Free Radicals ◽  
Reaction Kinetics ◽  
Phenolic Antioxidants ◽  
Biological Models ◽  
Kinetics Of
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