Adventitious Shoot Organogenesis and Plant Regeneration from Leaf and Cotyledon Explants ofCitrullus colocynthis

2014 ◽  
Vol 20 (3) ◽  
pp. 235-244
Author(s):  
T. Shasthree ◽  
D. Ramakrishna ◽  
M. A. Imran ◽  
Ch. Chandrashekar
Keyword(s):  
Plant Regeneration ◽  
Shoot Organogenesis ◽  
Adventitious Shoot ◽  
Cotyledon Explants ◽  
Adventitious Shoot Organogenesis

scholarly journals Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

2014 ◽  
Vol 31 (2) ◽  
pp. 115-121 ◽  
Author(s):  
Hao Huang ◽  
JunCheng Li ◽  
KunXi OuYang ◽  
XianHai Zhao ◽  
Pei Li ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Shoot Organogenesis ◽  
Adventitious Shoot ◽  
Cotyledon Explants ◽  
Adventitious Shoot Organogenesis

scholarly journals Adventitious Shoot Organogenesis and Plant Regeneration from Cotyledons of Tetraploid Watermelon

HortScience ◽  
1994 ◽  
Vol 29 (3) ◽  
pp. 211-213 ◽  
Author(s):  
Michael E. Compton ◽  
D.J. Gray
Keyword(s):  
Shoot Organogenesis ◽  
Citrullus Lanatus ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Mature Seed ◽  
Regeneration Medium ◽  
Breeding Lines ◽  
Cotyledon Explants ◽  
Explant Source ◽  
Adventitious Shoot Organogenesis

Cotyledon explants of four watermelon [Citrullus lanatus (Thunb.) Mataum. & Nakai] breeding lines (F92U8, SP90-1, SP90-2, and SP90-4) were prepared from mature seed or from 2-, 4-, 6-, 8-, or 10-day-old seedlings. Explants were incubated on shoot regeneration medium for 8 weeks followed by 4 weeks on shoot elongation medium. The four genotypes differed in their ability to produce shoots at each explant age. The highest frequency with which F92U8 (66%) and SP90-2 (60%) explants produced shoots was for 2-day-old seedlings. Fewer explants formed shoots when established from mature seed or seedlings older than 2 days. In contrast, the percentage of SP90-4 explants that produced shoots was highest when cotyledons were obtained from 4-day-old seedlings (40%), but the response was less than the optimum for F92U8 and SP90-2. SP90-1 cotyledon explants exhibited the poorest response of the four breeding lines (<11% produced shoots), with little difference in response among the explant ages tested. The number of shoots per responding explant also depended on the age of the explant source. Explants from 2- to 4-day-old seedlings produced the most shoots. Fewer shoots formed on cotyledons from mature seed or seedlings older than 4 days.

scholarly journals Adventitious Shoot Organogenesis and Plant Regeneration from Internode Explants of Paederia foetida L.:A Valuable Medicinal Plant

2017 ◽  
Vol 14 (3) ◽  
pp. 893-900
Author(s):  
Biswaranjan Behera ◽  
Shashikanta Behera ◽  
Padan K. Jena ◽  
Durga P. Barik ◽  
Soumendra K. Naik
Keyword(s):  
Plant Regeneration ◽  
Shoot Organogenesis ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Garden Soil ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Paederia Foetida ◽  
Adventitious Shoot Organogenesis ◽  
Internode Explants

ABSTRACT: A plant regeneration protocol via adventitious shoot organogenesis from internode explants of Paederia foetida (Skunk vine) is reported here for the first time. Three explants (leaf, mature internode and internode derived from axenic shoot cultures) were tested for shoot organogenesis. Leaf explants failed to induce adventitious shoots whereas axenic internode explant was found to be superior to mature internode explants for the induction of adventitious shoots. Axenic internode explants cultured on MS medium supplemented with 3.0 mg/l BAP showed maximum (86.7 %; 10.4 shoots per explant) adventitious shoot organogenesis. The regenerated shoots were best rooted (90 %; 14 roots per shoot) on half-strength MS medium. Eighty percent of the rooted shoots were successfully acclimatized in soil: sand (1:1) mixture. All these acclimatized plants were successfully transferred to larger pots containing garden soil and subsequently established in the field.

Adventitious shoot organogenesis and encapsulation technology in hop (Humulus lupulus L.)

2020 ◽  
Vol 270 ◽  
pp. 109416 ◽  
Author(s):  
Claudia Maria Liberatore ◽  
Margherita Rodolfi ◽  
Deborah Beghè ◽  
Andrea Fabbri ◽  
Tommaso Ganino ◽  
...  
Keyword(s):  
Shoot Organogenesis ◽  
Adventitious Shoot ◽  
Humulus Lupulus ◽  
Humulus Lupulus L ◽  
Adventitious Shoot Organogenesis

scholarly journals Gelling Agents Affect Adventitious Shoot Organogenesis in White Ash

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 873E-873
Author(s):  
Sharon A. Bates ◽  
John E. Preece ◽  
John H. Yopp
Keyword(s):  
Liquid Medium ◽  
Shoot Growth ◽  
Shoot Organogenesis ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Gelling Agent ◽  
White Ash ◽  
Shoot Initiation ◽  
Adventitious Shoot Organogenesis

Dissected white ash seeds were placed on an agar-solidified MS medium with 10 μM TDZ and 1 μM 2,4-D (shoot initiation medium). After 4 weeks, explants were transferred to shoot elongation medium (3 μM TDZ, 1 μM BA, and 1 μM IBA) solidified with 0.7% Sigma agar, 0.525% agar + 0.05% gelrite, 0.35% agar + 0.1% gelrite, 0.175% agar + 0.15% gelrite, 0.2% gelrite, or no gelling agent (liquid medium). After 12 weeks in vitro, shoot growth and number were suppressed in cultures containing 0.2% gelrite (9.3 mm and 0.7 shoots) and in cultures containing no gelling agent (6.9 mm and 0.7 shoots). There were no differences in shoot growth and number in cultures containing 0.7% Sigma agar (2.2 mm and 16.5 shoots), 0.525% agar + 0.05% gelrite (2.6 mm and 18.7 shoots), 0.35% agar + 0.1% gelrite (1.6 mm and 17.4 shoots), and 0.175% agar + 0.15% gelrite (2.0 mm and 20.4 shoots). The most vitrification occurred in cultures on medium with the lowest amount of agar, gelrite only, and liquid medium.

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