Endothelial Cell Monolayer Formation: Effect of Substrate and Fluid Shear Stress

Endothelium ◽  
2004 ◽  
Vol 11 (1) ◽  
pp. 29-44 ◽  
Author(s):  
S. M. Kladakis ◽  
R. M. Nerem
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Fluid Shear Stress ◽  
Cell Monolayer ◽  
Endothelial Cell Monolayer ◽  
Fluid Shear ◽  
Monolayer Formation

scholarly journals Turbulent fluid shear stress induces vascular endothelial cell turnover in vitro.

1986 ◽  
Vol 83 (7) ◽  
pp. 2114-2117 ◽  
Author(s):  
P. F. Davies ◽  
A. Remuzzi ◽  
E. J. Gordon ◽  
C. F. Dewey ◽  
M. A. Gimbrone
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Fluid Shear Stress ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Cell Turnover ◽  
Fluid Shear ◽  
Turbulent Fluid

Effect of Shear Stress on Platelet Adhesion to Expanded Polytetrafluoroethylene, a Silicone Sheet, and an Endothelial Cell Monolayer

ASAIO Journal ◽  
2000 ◽  
Vol 46 (6) ◽  
pp. 696-701 ◽  
Author(s):  
Katsuko Sakai Furukawa ◽  
Takashi Ushida ◽  
Hirohito Sugano ◽  
Tamotsu Tamaki ◽  
Norio Ohshima ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Platelet Adhesion ◽  
Cell Monolayer ◽  
Endothelial Cell Monolayer ◽  
Expanded Polytetrafluoroethylene ◽  
Silicone Sheet

scholarly journals Endothelial cell monolayer formation on a small-diameter vascular graft surface under pulsatile flow conditions

2021 ◽  
Vol 23 (3) ◽  
pp. 101-114
Author(s):  
M. Yu. Khanova ◽  
E. A. Velikanova ◽  
V. G. Matveeva ◽  
E. O. Krivkina ◽  
T. V. Glushkova ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Pulsatile Flow ◽  
Vascular Graft ◽  
Cell Monolayer ◽  
Small Diameter ◽  
Flow Conditions ◽  
Endothelial Cell Culture ◽  
Monolayer Formation ◽  
Whole Transcriptome

Objective: to create a cell-populated small-diameter vascular graft (SDVG) using autologous endothelial cells and extracellular matrix proteins, and to evaluate the efficiency of endothelial cell monolayer formation during shear stress preconditioning in a SDVG.Materials and methods. PHBV/PCL tubular scaffolds of vascular grafts were made by electrospinning from a mixture of polyhydroxybutyrate-valerate (PHBV) copolymer and polycaprolactone (PCL) and modified with fibrin. To populate the graft, an endothelial cell culture was isolated from the blood of patients with coronary heart disease. Phenotyping of endothelial colony-forming cell (ECFC) culture was performed by flow cytometry and immunofluorescence microscopy. Cell proliferative and angiogenic activity were also studied. Cell-populated vascular scaffolds were cultured in a pulsatile flow setup with a final shear stress of 2.85 dyne/cm2. The effect of pulsatile flow on monolayer formation was assessed by immunofluorescence, scanning electron microscopy, atomic force microscopy, and whole-transcriptome RNA sequencing.Results. Under the influence of pulsatile flow, endothelial cells that were seeded into the tubular scaffold showed an increase in the expression level of endothelial profile proteins, focal adhesion and cytoskeleton. In contrast to endothelial cell culture on a vascular graft surface under static conditions, when cultured under pulsatile flow with 2.85 dyne/ cm2 shear stress, endothelial lining cells have an increased ability to adhere and are oriented along the pulsatile flow path. Whole-transcriptome RNA sequencing showed that induced shear stress increased expression levels of differentially expressed genes encoding proteins that ensure vascular development, endothelial integrity, and endothelial metabolism. A protocol for fabrication of a personalized cell-populated biodegradable SDVG under pulsatile flow conditions was developed.Conclusion. The use of autologous fibrin and ECFC culture, as well as shear stress preconditioning, allow to obtain a personalized cell-populated SDVG with continuous functional endothelial monolayer adapted to the flow.

A mechanosensory complex that mediates the endothelial cell response to fluid shear stress

Nature ◽  
2005 ◽  
Vol 437 (7057) ◽  
pp. 426-431 ◽  
Author(s):  
Eleni Tzima ◽  
Mohamed Irani-Tehrani ◽  
William B. Kiosses ◽  
Elizabetta Dejana ◽  
David A. Schultz ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Response ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Endothelial Cell Response

Effects of Fluid Shear Stress on Endothelial Cell Invasion Into Three-Dimensional Matrices

2007 ◽  
Author(s):  
Hojin Kang ◽  
Kayla J. Bayless ◽  
Roland Kaunas
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Invasion ◽  
Fluid Shear Stress ◽  
Vascular Endothelial Cells ◽  
Umbilical Vein ◽  
Shear Stresses ◽  
Collagen Gels ◽  
Fluid Shear

We have previously developed a cell culture model to study the effects of angiogenic factors, such as sphingosine-1-phosphate (S1P), on the invasion of endothelial cells into the underlying extracellular matrix. In addition to biochemical stimuli, vascular endothelial cells are subjected to fluid shear stress due to blood flow. The present study is aimed at determining the effects of fluid shear stress on endothelial cell invasion into collagen gels. A device was constructed to apply well-defined fluid shear stresses to confluent human umbilical vein endothelial cells (HUVECs) seeded on collagen gels. Fluid shear stress induced significant increases in cell invasion with a maximal induction at ∼5 dyn/cm2. These results provide evidence that fluid shear stress is a significant stimulus for endothelial cell invasion and may play a role in regulating angiogenesis.

T-786C Polymorphism of thenos-3Gene and the Endothelial Cell Response to Fluid Shear Stress—A Proteome Analysis

2009 ◽  
Vol 8 (6) ◽  
pp. 3161-3168 ◽  
Author(s):  
Abdul R. Asif ◽  
Michael Oellerich ◽  
Victor William Armstrong ◽  
Markus Hecker ◽  
Marco Cattaruzza
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Response ◽  
Fluid Shear Stress ◽  
Proteome Analysis ◽  
Fluid Shear ◽  
Endothelial Cell Response

Glycated collagen alters endothelial cell actin alignment and nitric oxide release in response to fluid shear stress

2011 ◽  
Vol 44 (10) ◽  
pp. 1927-1935 ◽  
Author(s):  
Steven F. Kemeny ◽  
Dannielle S. Figueroa ◽  
Allison M. Andrews ◽  
Kenneth A. Barbee ◽  
Alisa Morss Clyne
Keyword(s):  
Nitric Oxide ◽  
Shear Stress ◽  
Endothelial Cell ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Nitric Oxide Release

Endothelial cell cytoskeletal alignment independent of fluid shear stress on micropatterned surfaces

2008 ◽  
Vol 371 (4) ◽  
pp. 787-792 ◽  
Author(s):  
Keri B. Vartanian ◽  
Sean J. Kirkpatrick ◽  
Stephen R. Hanson ◽  
Monica T. Hinds
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Fluid Shear Stress ◽  
Fluid Shear
Sign in / Sign up

Export Citation Format

Share Document