scholarly journals Marker-assisted selection and gene pyramiding for resistance to bacterial leaf blight disease of rice (Oryza sativa L.)

2019 ◽  
Vol 33 (1) ◽  
pp. 440-455 ◽  
Author(s):  
Samuel Chibuike Chukwu ◽  
Mohd Y. Rafii ◽  
Shairul Izan Ramlee ◽  
Siti Izera Ismail ◽  
Yussuf Oladosu ◽  
...  
Euphytica ◽  
2011 ◽  
Vol 187 (3) ◽  
pp. 313-323 ◽  
Author(s):  
R. K. Salgotra ◽  
B. B. Gupta ◽  
Reginald J. Millwood ◽  
Muthukumar Balasubramaniam ◽  
C. N. Stewart

Author(s):  
V. Srujana ◽  
M. Balram ◽  
B. Srinivas ◽  
N. Balram

Bacterial Leaf Blight disease epidemically damaged the rice crop. Absence of resistance against BLB disease in MTU1010 is one of the main reason for these epidemics. Thus, there is urgent need to search for resistant source and subsequently, their phenotypic evaluation to validate the resistant source. In this paper discussing about the highlights of phenotypic evaluation of Bacterial Leaf Blight resistance in MTU1010 NIL variety of rice. In the present study, fifty advanced breeding lines along with parents i.e., MTU1010 NIL X Akshayadhan NIL, were screened for bacterial leaf blight disease, Thirty-four breeding lines were shown resistance against bacterial leaf blight disease.


2018 ◽  
Vol 6 (2) ◽  
pp. 134
Author(s):  
Endang Kantikowati ◽  
Ridwan Haris ◽  
Saiful Anwar

The Experiment  was aimed to assess influence of concentration and application of biological agent Paenibacillus polymixa to bacterial leaf blight disease and  black rice yields  (Oryza sativa L.) local varieties The research was conducted in P4S Al Mukhlis   Kiangroke Village, Bandung District, West Java. In December 2016 until April 2017. The experiment used a split-plot design with two factor : 3 application period (W) as the main plot and 4 concentration level (K) as the subplot, thus obtained 12 treatment combinations and each combination was repeated 3 times. Then obtained 36 plot of experiment. Factor I main plot (W) : W1 = morning application (10, 20, 30, 40, 50, 60, 70 days after planting), W2 = daytime (10, 20, 30, 40, 50, 60, 70 days after planting), W3 = afternoon application (10, 20, 30, 40, 50, 60, 70 days after planting) and factor II (K) : K1 = Control, K2 = 5 ml L-1, K3 = 10 ml L-1, K4 = 15 ml L-1. The result showed that the effect of concentration  and time of application of biological agent of Paenibacillus polymixa gave no significant effect on plant height, disease intensity, number of productive tillers, number of the dry grain harvest, number of unhulled grain per panicle, and weight of 1000 filled grains. There was an interaction between concentration and time of application of biological agent of Paenibacillus polymixa to bacterial leaf blight disease intensity at age of 95 DAP, treatment of K3 (10ml L-1) with W1 (morning application)   gave more resistance effect to bacterial leaf blight intensity. 


2018 ◽  
Vol 35 (1) ◽  
pp. 113-120
Author(s):  
A. Acharya ◽  
N. R. Adhikari ◽  
R. B. Amgain ◽  
A. Poudel ◽  
R. Yadav ◽  
...  

 Bacterial blight disease of rice is a growing and challenging concern in Nepal. Since bacterial pathogen (Xanthomonas oryzae pv. oryzae) is difficult to manage by other means effectively, development of host plant resistance is the most effective mean to control this disease. This study was carried out to identify the bacterial leaf blight resistant genotypes of rice by using of molecular markers linked with bacterial blight resistant genes. Sixty genotypes of rice were screened at glass house for BB resistance and they were tested for the presence of Xa4, Xa5, Xa7 and Xa21 genes using markers MP, RM122, M5 and pTA248 respectively. IRBB 60 and Jumli Marshi were used as resistant and susceptible check respectively. Twenty five genotypes of rice were detected with presence of Xa4 gene, 24 genotypes with Xa5 gene and fourteen genotypes with Xa7 gene. Twenty four genotypes did not showed presence of any gene. Twenty four genotypes showed the presence of more than one gene with the specific molecular markers. Almost all genotypes that did not show presence of any gene were found highly susceptible in greenhouse conditions with both inoculums. Genotypes having more than one BB resistance gene were found resistant in greenhouse conditions with both inoculums suggesting combination of BB resistance gene through gene pyramiding will provide BB resistant varieties in rice breeding.


2015 ◽  
Vol 4 (1) ◽  
pp. 12-17
Author(s):  
Katelyn Joy Horgan ◽  
Jeffrey Odell Henderson

Rice (Oryza sativa) is a major staple crop around the world, particularly in developing regions such as Africa and Asia.  Attack of the rice crop by pathogens has the capacity to greatly diminish crop yield with major agricultural, economical and human consequences.  Xanthomonas orzyza pv. oryzae (Xoo), the causative agent of bacterial leaf blight, has the ability to cause substantial crop loss, up to 80% worldwide.  Genetic sources of resistance have long been considered the most sustainable method of disease control, both from an environmental and agricultural perspective.  This review considers the discovery, evolution, characterization and utilization of resistance genes (R genes) in rice against Xoo. R genes for Xoo are plentiful and exist at multiple loci throughout the rice genome due to multiple gene duplication and transposition events.  The presence of multiple gene loci makes gene pyramiding and resistance stacking accessible and fruitful applications in breeding for resistance.  Biotechnological approaches to take advantage of R gene function have also begun to take root.  However, a more global and concerted effort to study the multiple varieties and climates in which rice is grown is required in order to truly attain food security and find sustainable resistance.


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