scholarly journals Effect of High Glucose Concentration on Collagen Synthesis and Cholesterol Level in the Phenotypic Modulation of Aortic Cultured Smooth Muscle Cells of Sand Rat (Psammomys obesus)

2004 ◽  
Vol 5 (3) ◽  
pp. 227-235 ◽  
Author(s):  
S. Aouichat Bouguerra ◽  
Y. Benazzoug ◽  
F. Bekkhoucha ◽  
M. C. Bourdillon
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cholesterol Level ◽  
High Glucose ◽  
Glucose Concentration ◽  
Collagen Synthesis ◽  
Muscle Cells ◽  
High Glucose Concentration ◽  
Collagen Biosynthesis ◽  
Cellular Cholesterol

To simulate diabetic conditions, the effects of high glucose concentration on collagen synthesis and cholesterol level in cultured aortic smooth muscle cells ofPsammomyswere investigated. For collagen biosynthesis, smooth muscle cells (SMCs) were incubated in synthetic proliferative phase and in postconfluent phase withH3-proline. Cellular cholesterol was determined by enzymatic method. Under high glucose concentration, the results showed morphological modifications characterized by morphometric cellular, nuclear, and nucleolar changes. In biochemical studies, the authors observed an increase of free and esterified cellular cholesterol as well as of total proteins, collagen biosynthesis, andα1 (I+III) andα2 (I) chains of collagen contained in the SMCs and in the extracellular matrix. These results showed the sensitivity ofPsammomysaortic SMCs to high glucose concentration and would constitute an interesting cellular model to study atherosclerosis pathogeny in experimental diabetes.

Vasopressin activates Akt/mTOR pathway in smooth muscle cells cultured in high glucose concentration

2013 ◽  
Vol 441 (4) ◽  
pp. 923-928 ◽  
Author(s):  
Daniela K. Montes ◽  
Marianne Brenet ◽  
Vanessa C. Muñoz ◽  
Patricia V. Burgos ◽  
Carolina I. Villanueva ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
High Glucose ◽  
Glucose Concentration ◽  
Muscle Cells ◽  
Mtor Pathway ◽  
High Glucose Concentration ◽  
Cells Cultured

High glucose inhibits apoptosis in human coronary artery smooth muscle cells by increasing bcl-xL and bfl-1/A1

2002 ◽  
Vol 283 (2) ◽  
pp. C422-C428 ◽  
Author(s):  
Hiroya Sakuma ◽  
Mayumi Yamamoto ◽  
Mie Okumura ◽  
Toshihiro Kojima ◽  
Takako Maruyama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Coronary Artery ◽  
Smooth Muscle Cells ◽  
High Glucose ◽  
Glucose Concentration ◽  
Diabetic Patients ◽  
High Glucose Concentration ◽  
Human Coronary Artery ◽  
Normal Glucose ◽  
Apoptosis Regulation

Cardiovascular disease is a serious complication in diabetic patients. To elucidate the precise mechanisms of atherosclerosis in diabetic patients, the effects of high glucose concentration (25 mM) on apoptosis regulation and bcl-2 family protein expression in human coronary artery smooth muscle cells (CASMC) were examined. Treatment with a high level of glucose (25 mM) caused a significant decrease in apoptosis in CASMC compared with the same cells treated with a physiologically normal glucose concentration (5.5 mM) (23.9 ± 2.4% vs. 16.5 ± 1.8%; P < 0.01). With respect to apoptosis regulation, treatment of CASMC with high glucose concentration markedly increased mRNA expressions of bcl-xL and bfl-1/A1 compared with cells treated with normal glucose. High glucose induced phosphorylation of phosphatidylinositol 3-kinase (PI 3-K) and extracellular signal-regulated kinase (ERK)1/2 along with bcl-xL and bfl-1/A1 upregulation. These results suggest that high glucose suppresses apoptosis via upregulation of bcl-xL and bfl-1/A1 levels through PI 3-K and ERK1/2 pathways in CASMC. High glucose-induced increase in the expression of antiapoptotic proteins may be important in the development of atherosclerosis in diabetic patients.

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