scholarly journals Isolation and characterization of monoclonal antibodies against human carbonic anhydrase-IX

mAbs ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Anne E.G. Lenferink ◽  
Paul C. McDonald ◽  
Christiane Cantin ◽  
Suzanne Grothé ◽  
Mylene Gosselin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Carbonic Anhydrase ◽  
Carbonic Anhydrase Ix ◽  
Isolation And Characterization ◽  
Human Carbonic Anhydrase

Production and Characterization of Monoclonal Antibodies to Human Carbonic Anhydrase III

Hybridoma ◽  
1998 ◽  
Vol 17 (6) ◽  
pp. 553-558 ◽  
Author(s):  
HASSAN M.E. AZZAZY ◽  
PATRICK J. CUMMINGS ◽  
DAVID R. AMBROZAK ◽  
ROBERT H. CHRISTENSON
Keyword(s):  
Monoclonal Antibodies ◽  
Carbonic Anhydrase ◽  
Carbonic Anhydrase Iii ◽  
Human Carbonic Anhydrase

scholarly journals Hypoxia-inducible expression of the mouse carbonic anhydrase IX demonstrated by new monoclonal antibodies

2007 ◽  
Author(s):  
Miriam Zatovicova ◽  
Silvia Pastorekova ◽  
Jaromir Pastorek ◽  
Seppo Parkkila ◽  
Alzbeta Hulikova ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Carbonic Anhydrase ◽  
Inducible Expression ◽  
Carbonic Anhydrase Ix

scholarly journals Sortase-mediated site-specific modification of interleukin-2 for the generation of a tumor targeting acetazolamide-cytokine conjugate

2020 ◽  
Author(s):  
Baptiste Gouyou ◽  
J Millul ◽  
A Villa ◽  
S Cazzamalli ◽  
D Neri ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Tumor Targeting ◽  
Interleukin 2 ◽  
Industrial Applications ◽  
Carbonic Anhydrase Ix ◽  
Site Specific ◽  
Tumor Bearing ◽  
Bioactive Proteins

1.AbstractSmall ligands specific to tumor-associated antigens can be used as alternatives to antibodies for the delivery of small payloads such as radionuclides, cytotoxic drugs and fluorophores. Their use as delivery moiety of bioactive proteins like cytokines remains largely unexplored. Here, we describe the preparation and in vivo characterization of the first small molecule-cytokine conjugate targeting carbonic anhydrase IX (CAIX), a marker of renal cell carcinoma and hypoxia. Site-specific conjugation between interleukin-2 and acetazolamide was obtained by Sortase A-mediated transpeptidation. Binding of the conjugate to the cognate CAIX antigen was confirmed by surface plasmon resonance. The in vivo targeting of structures expressing carbonic anhydrase IX was assessed by biodistribution experiments in tumor bearing mice. Optimization of manufacturability and tumor targeting performance of acetazolamide-cytokine products will be required in order to enable industrial applications.Graphical abstract

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