Adipocyte hyperplasia: the primary mechanism of supraspinatus intramuscular fat accumulation after a complete rotator cuff tendon tear: a study in the rabbit

Aim: We investigated the therapeutic effects and optimal dose of human umbilical cord blood (UCB)-derived mesenchymal stem cell (MSC) injection in a chronic full-thickness rotator cuff tendon tear. Methods: Rabbits (n = 30) were allocated into three groups (normal saline, G1-Sal; 1 × 106 cells UCB-MSC, G2-Low; 2 × 106 cells UCB-MSC, G3-High). Injections were done into the chronic full-thickness rotator cuff tendon tear 6 weeks after a full-thickness tendon tear of the subscapularis was created. Gross & histologic evaluation and motion analysis was done at pre and 4 weeks post-injection. Results: There were no significant differences in tear size and motion analysis parameters 4 weeks after injection between G2-Low and G3-High. Conclusion: The benefits of UCB-MSCs are not dose-dependent in a rabbit model.

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Synergetic effects of shock waves with polydeoxyribonucleotides on rotator cuff tendon tear in a rabbit model

BIOCELL ◽

10.32604/biocell.2021.014350 ◽

2021 ◽

Vol 45 (3) ◽

pp. 527-536

Author(s):

DONG HAN KIM ◽

DONG RAK KWON ◽

GI-YOUNG PARK ◽

YONG SUK MOON

Keyword(s):

Rotator Cuff ◽

Shock Waves ◽

Rabbit Model ◽

Tendon Tear ◽

Rotator Cuff Tendon ◽

Synergetic Effects

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Therapeutic Effects of Umbilical Cord Blood-Derived Mesenchymal Stem Cells Combined with Polydeoxyribonucleotides on Full-Thickness Rotator Cuff Tendon Tear in a Rabbit Model

Cell Transplantation ◽

10.1177/0963689718799040 ◽

2018 ◽

Vol 27 (11) ◽

pp. 1613-1622 ◽

Cited By ~ 4

Author(s):

Dong Rak Kwon ◽

Gi-Young Park ◽

Yong Suk Moon ◽

Sang Chul Lee

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Rotator Cuff ◽

Cord Blood ◽

Umbilical Cord Blood ◽

Rabbit Model ◽

Full Thickness ◽

Tendon Tear ◽

Rotator Cuff Tendon ◽

Significant Difference

While therapies using mesenchymal stem cells (MSCs) to treat rotator cuff tendon tear (RCTT) have yielded some promising preliminary results, MSCs therapy has not yet completely regenerated full-thickness RCTT (FTRCTT). It has recently been reported that polydeoxyribonucleotide (PDRN) is effective in the treatment of chronic rotator cuff disease. We hypothesized that local injection of human umbilical cord blood-derived (UCB)–MSCs with PDRN would be more effective in regenerating tendon tear than UCB-MSCs alone. The purpose of this study was to evaluate the effects of UCB–MSCs combined with different doses of PDRN on the regeneration of RCTT in a chronic RCTT model by using a rabbit model. New Zealand white rabbits ( n = 24) with FTRCTT were allocated randomly into three groups (8 rabbits per group). Three different injectants (G1-S, 0.2 mL UCB-MSCs; G2-P1, 0.2 mL UCB-MSCs with one injection of 0.2 mL PDRN; G3-P4, 0.2 mL UCB-MSCs, and four injections of 0.2 mL PDRN per week) were injected into FTRCTT under US-guidance. After the rabbits were euthanized, we evaluated ross morphological and histological change. Motion analysis was also performed. There were significant differences in gross morphological changes between before, and at 4 weeks after injection, in all three groups, but no differences were found among the three groups. Masson’s trichrome (MT) or anti-type 1 collagen antibody (COL-1)-positive cell densities in G2-P1 and G3-P4 were improved significantly compared with those in G1-S, but showed no significant difference between G2-P1 and G3-P4. On motion analysis, walking distance and fast walking time in G2-P1 and G3-P4 were significantly longer/higher than those in G1-S, but showed no significant differences between G2-P1 and G3-P4. These results demonstrated that there was no significant difference in the gross morphologic change of tendon tear between UCB-MSCs only and combination with PDRN injection in rabbit model of chronic traumatic FTRCTT. Furthermore, there were no significant differences in the regenerative effects between high and low doses of (0.8 and 0.2) mL of PDRN.

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Regeneration of Full-Thickness Rotator Cuff Tendon Tear After Ultrasound-Guided Injection With Umbilical Cord Blood-Derived Mesenchymal Stem Cells in a Rabbit Model

Stem Cells Translational Medicine ◽

10.5966/sctm.2015-0040 ◽

2015 ◽

Vol 4 (11) ◽

pp. 1344-1351 ◽

Cited By ~ 35

Author(s):

Gi-Young Park ◽

Dong Rak Kwon ◽

Sang Chul Lee

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Rotator Cuff ◽

Cord Blood ◽

Umbilical Cord Blood ◽

Rabbit Model ◽

Full Thickness ◽

Ultrasound Guided ◽

Tendon Tear ◽

Rotator Cuff Tendon

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Editorial Commentary: Look More Closely at those Coronal Magnetic Resonance Imaging Cuts Before Concluding a Rotator Cuff Tendon Tear Is Irreparable—Don’t Let an L-Shaped Tear Fool You

Arthroscopy The Journal of Arthroscopic and Related Surgery ◽

10.1016/j.arthro.2020.08.027 ◽

2020 ◽

Vol 36 (11) ◽

pp. 2831

Author(s):

Josef K. Eichinger

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Rotator Cuff ◽

Resonance Imaging ◽

Tendon Tear ◽

Rotator Cuff Tendon ◽

Editorial Commentary

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Effect of Bone Marrow Aspirate Concentrate-Platelet-Rich Plasma on Tendon-Derived Stem Cells and Rotator Cuff Tendon Tear

Cell Transplantation ◽

10.3727/096368917x694705 ◽

2017 ◽

Vol 26 (5) ◽

pp. 867-878 ◽

Cited By ~ 24

Author(s):

Sun Jeong Kim ◽

Da Hyun Song ◽

Jong Wook Park ◽

Silvia Park ◽

Sang Jun Kim

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Rotator Cuff ◽

Bone Marrow Aspirate ◽

Platelet Rich Plasma ◽

Immunocytochemical Staining ◽

Osteogenic Potential ◽

Tendon Tear ◽

Rotator Cuff Tendon ◽

Significant Difference

Bone marrow aspirate concentrates (BMACs) and platelet-rich plasma (PRP) are good sources to control the differentiation of tendon-derived stem cells (TDSCs), but there has been no study about the effect of the BMAC–PRP complex on TDSCs and tendinopathy. The aim of this study was to investigate the effect of BMAC–PRP on the TDSCs and to find the therapeutic effect of BMAC–PRP on the rotator cuff tendon tear. The chondrogenic and osteogenic potential of TDSCs decreased, but the adipogenic potential of TDSCs revealed no significant difference when they were cocultured with BMAC–PRP. Cell proliferation was significantly greater in TDSCs cocultured with BMAC–PRP than in TDSCs. The degree of wound closure (percentage) was different between TDSCs and TDSCs with BMAC–PRP. There was no significant difference in expression of collagen type I and type III in immunocytochemical staining in the presence of BMAC–PRP. Initial visual analog scale (VAS) score was 5.8±1.9, which changed to 5.0±2.3 at 3 weeks and 2.8±2.3 at 3 months after the BMAC–PRP injection ( p<0.01). The American Shoulder Elbow Surgeon score changed from 39.4±13.0 at baseline to 52.9±22.9 at 3 weeks and 71.8±19.7 at 3 months after the injection ( p<0.01). The initial torn area of the rotator cuff tendon was 30.2±24.5 mm2, and this area was reduced to 22.5±18.9 mm2 at 3 months, but the change was not significant ( p > 0.05). The data indicate that BMAC–PRP enhances the proliferation and migration of TDSCs and prevents the aberrant chondrogenic and osteogenic differentiation of TDSCs, which might provide a mechanistic basis for the therapeutic benefits of BMAC–PRP for rotator cuff tendon tear.

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