Studies on Mimic Peroxidase Behaviors of Molybdenum(VI)–Sodium Dodecyl Sulphate Complex for the Determination of Hydrogen Peroxide and Glucose

2004 ◽  
Vol 37 (4) ◽  
pp. 561-573 ◽  
Author(s):  
Li‐Hua Chen ◽  
Liu‐Zhan Liu ◽  
Han‐Xi Shen
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl

THE SULPHYDRYL CONTENT OF β-LACTOGLOBULIN

1960 ◽  
Vol 38 (3) ◽  
pp. 269-274 ◽  
Author(s):  
A. F. S. A. Habeeb
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Iodoacetic Acid ◽  
Urea Denaturation ◽  
Denatured Protein ◽  
A Value ◽  
Sulphydryl Groups ◽  
Β Lactoglobulin

The reaction of sulphydryl groups of β-lactoglobulin with N-ethylmaleimide, p-chloromercuribenzoate, and iodoacetic acid has been studied. Using N-ethylmaleimide with native and sodium dodecyl sulphate denatured protein, a value of 2.3 groups of sulphydryl per mole of protein was obtained. A value of 2.15 moles of sulphydryl per mole of protein was obtained from reaction with p-chloromercuribenzoate but lower values were obtained after urea denaturation. The reaction of iodoacetic acid was followed by the determination of residual sulphydryl by the p-chloromercuribenzoate method.

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