scholarly journals Characterization of the translocon of the outer envelope of chloroplasts

2003 ◽  
Vol 160 (4) ◽  
pp. 541-551 ◽  
Author(s):  
Enrico Schleiff ◽  
Jürgen Soll ◽  
Michael Küchler ◽  
Werner Kühlbrandt ◽  
Roswitha Harrer

The protein translocon of the outer envelope of chloroplasts (Toc) consists of the core subunits Toc159, Toc75, and Toc34. To investigate the molecular structure, the core complex was purified. This core complex has an apparent molecular mass of ∼500 kD and a molecular stoichiometry of 1:4:4–5 between Toc159, Toc75, and Toc34. The isolated translocon recognizes both transit sequences and precursor proteins in a GTP-dependent manner, suggesting its functional integrity. The complex is embedded by the lipids phosphatidylcholine and digalactosyldiacylglyceride. Two-dimensional structural analysis by EM revealed roughly circular particles consistent with the formation of a stable core complex. The particles show a diameter of ∼130 Å with a solid ring and a less dense interior structure. A three-dimensional map obtained by random conical tilt reconstruction of electron micrographs suggests that a “finger”-like central region separates four curved translocation channels within one complex.

Author(s):  
Rayya A. Al Balushi ◽  
Muhammad S. Khan ◽  
Md. Serajul Haque Faizi ◽  
Ashanul Haque ◽  
Kieran Molloy ◽  
...  

In the crystal structure of the title compound, [Cu4Cl6O(C13H9N)4]·CH2Cl2, the core molecular structure consists of a Cu4 tetrahedron with a central interstitial O atom. Each edge of the Cu4 tetrahedron is bridged by a chlorido ligand. Each copper(II) cation is coordinated to the central O atom, two chlorido ligands and one N atom of the 4-phenylethynylpyridine ligand. In the crystal, the molecules are linked by intermolecular C—H...Cl interactions. Furthermore, C—H...π and π–π interactions also connect the molecules, forming a three-dimensional network. Hirshfeld surface analysis indicates that the most important contributions for the packing arrangement are from H...H and C...H/H...C interactions.


2008 ◽  
Vol 16 (4) ◽  
Author(s):  
T. Antosiewicz ◽  
T. Szoplik

AbstractIn a previous paper we proposed a modification of metal-coated tapered-fibre aperture probes for scanning near-field optical microscopes (SNOMs). The modification consists in radial corrugations of the metal-dielectric interface oriented inward the core. Their purpose is to facilitate the excitation of surface plasmons, which increase the transport of energy beyond the cut-off diameter and radiate a quasi-dipolar field from the probe output rim. An increase in energy output allows for reduction of the apex diameter, which is the main factor determining the resolution of the microscope. In two-dimensional finite-difference time-domain (FDTD) simulations we analyse the performance of the new type of SNOM probe. We admit, however, that the two-dimensional approximation gives better results than expected from exact three-dimensional ones. Nevertheless, optimisation of enhanced energy throughput in corrugated probes should lead to at least twice better resolution with the same sensitivity of detectors available nowadays.


2017 ◽  
Vol 73 (5) ◽  
pp. 407-413 ◽  
Author(s):  
Balasubramanian Sridhar ◽  
Jagadeesh Babu Nanubolu ◽  
Krishnan Ravikumar ◽  
Govindaraju Karthik ◽  
Basi Venkata Subba Reddy

Isostructurality is more likely to occur in multicomponent systems. In this context, three closely related solvates were crystallized, namely, benzene (C27H21BrO6·C6H6), toluene (C27H21BrO6·C7H8) and xylene (C27H21BrO6·C8H10) with methyl 3a-acetyl-3-(4-bromophenyl)-4-oxo-1-phenyl-3,3a,4,9b-tetrahydro-1H-furo[3,4-c]chromene-1-carboxylate, and their crystal structures determined. All three structures belong to the same space group (P\overline{1}) and display similar unit-cell dimensions and conformations, as well as isostructural crystal packings. The isostructurality is confirmed by unit-cell and isostructural similarity indices. In each solvate, weak C—H...O and C—H...π interactions extend the molecules into two-dimensional networks, which are further linked by C—H...Br and Br...Br interactions into three-dimensional networks. The conformation of the core molecule is predominantly responsible for governing the isostructurality.


Kelvin showed that a two-dimensional vortex under a two-dimensional disturbance in incompressible flow responds at a discrete set of eigenvalues, which were found by Broadbent & Moore ( Phil. Trans. R. Soc. Lond. A 290, 353-371 (1979) to become unstable in a compressible fluid. It is now shown that three-dimensional perturbations are also unstable provided the wavelength is greater than some critical value that depends on the Mach number of the vortex. A critical boundary dividing stable from unstable modes is defined. Most of the results relate to a Rankine vortex, as in the previous work mentioned above, but some results are also given for a vortex with a different velocity profile within the core; qualitatively the same kind of behaviour is found.


Author(s):  
Graeme W Milton ◽  
Pierre Seppecher

We give a complete characterization of the possible response matrices at a fixed frequency of n -terminal electrical networks of inductors, capacitors, resistors and grounds, and of n -terminal discrete linear elastodynamic networks of springs and point masses, both in three-dimensional and two-dimensional cases. Specifically, we construct networks that realize any response matrix that is compatible with the known symmetry properties and thermodynamic constraints of response matrices. Owing to a mathematical equivalence, we also obtain a characterization of the response matrices of discrete acoustic networks.


1996 ◽  
Vol 449 ◽  
Author(s):  
R. Di Felice ◽  
J. E. Northrup ◽  
J. Neugebauer

ABSTRACTWe present a first-principles characterization of the initial stages of formation of AlN films on c-plane SiC substrates. Studying the competition between two-dimensional films and three-dimensional islands as a function of Al and N abundances, we find that a two-dimensional film can wet the surface in N-rich conditions. Ordered layer-by-layer growth can proceed to some extent on this wetting layer, and is improved by the formation of an atomically mixed interface which eliminates interface charge accumulation. Our results indicate that the stable AlN films grow in the (0001) orientation on the Si-terminated SiC(0001) substrate.


2013 ◽  
Vol 19 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Miquella G. Chavez ◽  
Wenli Yu ◽  
Brian Biehs ◽  
Hidemitsu Harada ◽  
Malcolm L. Snead ◽  
...  

Author(s):  
C. Rogers ◽  
T. Ruggeri ◽  
W. K. Schief

A classical system of conservation laws descriptive of relativistic gasdynamics is examined. In the two-dimensional stationary case, the system is shown to be invariant under a novel multi-parameter class of reciprocal transformations. The class of invariant transformations originally obtained by Bateman in non-relativistic gasdynamics in connection with lift and drag phenomena is retrieved as a reduction in the classical limit. In the general 3+1-dimensional case, it is demonstrated that Synge’s geometric characterization of the pressure being constant along streamlines encapsulates a three-dimensional extension of an integrable Heisenberg spin equation.


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