High-resolution brain tumor visualization using three-dimensional x-ray phase contrast tomography

2007 ◽  
Vol 52 (23) ◽  
pp. 6923-6930 ◽  
Author(s):  
F Pfeiffer ◽  
O Bunk ◽  
C David ◽  
M Bech ◽  
G Le Duc ◽  
...  
Keyword(s):  
Brain Tumor ◽  
High Resolution ◽  
Phase Contrast ◽  
Three Dimensional ◽  
X Ray ◽  
Tumor Visualization ◽  
Phase Contrast Tomography

scholarly journals Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography

2018 ◽  
Vol 115 (27) ◽  
pp. 6940-6945 ◽  
Author(s):  
Mareike Töpperwien ◽  
Franziska van der Meer ◽  
Christine Stadelmann ◽  
Tim Salditt
Keyword(s):  
Phase Contrast ◽  
Spatial Organization ◽  
Granular Layer ◽  
Three Dimensional ◽  
Cell Segmentation ◽  
X Ray ◽  
Human Cerebellum ◽  
Conventional Histology ◽  
3D Information ◽  
Phase Contrast Tomography

To quantitatively evaluate brain tissue and its corresponding function, knowledge of the 3D cellular distribution is essential. The gold standard to obtain this information is histology, a destructive and labor-intensive technique where the specimen is sliced and examined under a light microscope, providing 3D information at nonisotropic resolution. To overcome the limitations of conventional histology, we use phase-contrast X-ray tomography with optimized optics, reconstruction, and image analysis, both at a dedicated synchrotron radiation endstation, which we have equipped with X-ray waveguide optics for coherence and wavefront filtering, and at a compact laboratory source. As a proof-of-concept demonstration we probe the 3D cytoarchitecture in millimeter-sized punches of unstained human cerebellum embedded in paraffin and show that isotropic subcellular resolution can be reached at both setups throughout the specimen. To enable a quantitative analysis of the reconstructed data, we demonstrate automatic cell segmentation and localization of over 1 million neurons within the cerebellar cortex. This allows for the analysis of the spatial organization and correlation of cells in all dimensions by borrowing concepts from condensed-matter physics, indicating a strong short-range order and local clustering of the cells in the granular layer. By quantification of 3D neuronal “packing,” we can hence shed light on how the human cerebellum accommodates 80% of the total neurons in the brain in only 10% of its volume. In addition, we show that the distribution of neighboring neurons in the granular layer is anisotropic with respect to the Purkinje cell dendrites.

scholarly journals Synchrotron Radiation X-Ray Phase-Contrast Tomography Visualizes Microvasculature Changes in Mice Brains after Ischemic Injury

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Miao ◽  
Zhixia Wu ◽  
Miao Li ◽  
Yuanyuan Ji ◽  
Bohua Xie ◽  
...  
Keyword(s):  
High Resolution ◽  
Synchrotron Radiation ◽  
Contrast Agent ◽  
Phase Contrast ◽  
Cerebrovascular Diseases ◽  
Intact Mouse ◽  
X Ray ◽  
Brain Vasculature ◽  
Phase Contrast Tomography ◽  
Brain Microvasculature

Imaging brain microvasculature is important in plasticity studies of cerebrovascular diseases. Applying contrast agents, traditionalμCT andμMRI methods gain imaging contrast for vasculature. The aim of this study is to develop a synchrotron radiation X-ray inline phase-contrast tomography (SRXPCT) method for imaging the intact mouse brain (micro)vasculature in high resolution (~3.7 μm) without contrast agent. A specific preparation protocol was proposed to enhance the phase contrast of brain vasculature by using density difference over gas-tissue interface. The CT imaging system was developed and optimized to obtain 3D brain vasculature of adult male C57BL/6 mice. The SRXPCT method was further applied to investigate the microvasculature changes in mouse brains (n=14) after 14-day reperfusion from transient middle cerebral artery occlusion (tMCAO). 3D reconstructions of brain microvasculature demonstrated that the branching radius ratio (post- to preinjury) of small vessels (radius < 7.4 μm) in the injury group was significantly smaller than that in the sham group (p<0.05). This result revealed the active angiogenesis in the recovery brain after stroke. As a high-resolution and contrast-agent-free method, the SRXPCT method demonstrates higher potential in investigations of functional plasticity in cerebrovascular diseases.

scholarly journals Noninvasive 3D Structural Analysis of Arthropod by Synchrotron X-Ray Phase Contrast Tomography

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Shengkun Yao ◽  
Yunbing Zong ◽  
Jiadong Fan ◽  
Zhibin Sun ◽  
Huaidong Jiang
Keyword(s):  
Phase Contrast ◽  
Phase Retrieval ◽  
High Efficiency ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Circulatory System ◽  
Low Contrast ◽  
X Ray ◽  
Potential Applications ◽  
Phase Contrast Tomography

X-ray imaging techniques significantly advanced our understanding of materials and biology, among which phase contrast X-ray microscopy has obvious advantages in imaging biological specimens which have low contrast by conventional absorption contrast microscopy. In this paper, three-dimensional microstructure of arthropod with high contrast has been demonstrated by synchrotron X-ray in-line phase contrast tomography. The external morphology and internal structures of an earthworm were analyzed based upon tomographic reconstructions with and without phase retrieval. We also identified and characterized various fine structural details such as the musculature system, the digestive system, the nervous system, and the circulatory system. This work exhibited the high efficiency, high precision, and wide potential applications of synchrotron X-ray phase contrast tomography in nondestructive investigation of low-density materials and biology.

Three-dimensional morphology of cementite in steel studied by X-ray phase-contrast tomography

2012 ◽  
Vol 67 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Alexander Kostenko ◽  
Hemant Sharma ◽  
E. Gözde Dere ◽  
Andrew King ◽  
Wolfgang Ludwig ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Three Dimensional ◽  
X Ray ◽  
Phase Contrast Tomography

scholarly journals Multiscale three-dimensional imaging of intact human organs down to the cellular scale using hierarchical phase-contrast tomography

2021 ◽  
Author(s):  
C. Walsh ◽  
P. Tafforeau ◽  
Willi L. Wagner ◽  
D. J. Jafree ◽  
A. Bellier ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Soft Tissues ◽  
Structural Information ◽  
Three Dimensional ◽  
High Energy ◽  
X Ray ◽  
Human Organs ◽  
Health And Disease ◽  
Functional Units ◽  
Phase Contrast Tomography

ABSTRACTHuman organs are complex, three-dimensional and multiscale systems. Spatially mapping the human body down through its hierarchy, from entire organs to their individual functional units and specialised cells, is a major obstacle to fully understanding health and disease. To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique utilising the European Synchrotron Radiation Facility’s Extremely Brilliant Source: the world’s first high-energy 4th generation X-ray source. HiP-CT enabled three-dimensional and non-destructive imaging at near-micron resolution in soft tissues at one hundred thousand times the voxel size whilst maintaining the organ’s structure. We applied HiP-CT to image five intact human parenchymal organs: brain, lung, heart, kidney and spleen. These were hierarchically assessed with HiP-CT, providing a structural overview of the whole organ alongside detail of the organ’s individual functional units and cells. The potential applications of HiP-CT were demonstrated through quantification and morphometry of glomeruli in an intact human kidney, and identification of regional changes to the architecture of the air-tissue interface and alveolar morphology in the lung of a deceased COVID-19 patient. Overall, we show that HiP-CT is a powerful tool which can provide a comprehensive picture of structural information for whole intact human organs, encompassing precise details on functional units and their constituent cells to better understand human health and disease.

scholarly journals A computational platform for the virtual unfolding of Herculaneum Papyri

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sara Stabile ◽  
Francesca Palermo ◽  
Inna Bukreeva ◽  
Daniela Mele ◽  
Vincenzo Formoso ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Three Dimensional ◽  
Complicated Structure ◽  
X Ray ◽  
Challenging Tasks ◽  
Comprehensive Survey ◽  
Phase Contrast Tomography ◽  
Computational Platform ◽  
The Way

AbstractAncient Herculaneum papyrus scrolls, hopelessly charred in the 79 A.D. Vesuvius eruption, contain valuable writings of the Greek philosophers of the day, including works of the Epicurean Philodemus. X-ray phase contrast tomography has recently begun unlocking their secrets. However, only small portions of the text hidden inside the scroll have been recover. One of the challenging tasks in Herculaneum papyri investigation is their virtual unfolding because of their highly complicated structure and three-dimensional arrangement. Although this procedure is feasible, problems in segmentation and flattening hinder the unrolling of a large portion of papyrus. We propose a computational platform for the virtual unfolding procedure, and we show the results of its application on two Herculaneum papyrus fragments. This work paves the way to a comprehensive survey and to further interpretation of larger portions of text hidden inside the carbonized Herculaneum papyri.

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