Real-time transport kinetics of drug encapsulated nanoparticles into apoptotic cancer cells inside microchannels

2021 ◽  
Vol 32 (50) ◽  
pp. 505704
Author(s):  
Surjendu Maity ◽  
Tamanna Bhuyan ◽  
Jagannath Prasad Pattanayak ◽  
Siddhartha Sankar Ghosh ◽  
Dipankar Bandyopadhyay
Keyword(s):  
Cancer Cells ◽  
Real Time ◽  
Transport Kinetics ◽  
Kinetics Of ◽  
Encapsulated Nanoparticles

scholarly journals Differential toxicity of gold-doxorubicin in cancer cells vs. cardiomyocytes as measured by real-time growth assays and fluorescence lifetime imaging microscopy (FLIM)

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5732-5741 ◽  
Author(s):  
Eric Tawagi ◽  
Charlotte Massmann ◽  
Hicham Chibli ◽  
Jay L. Nadeau
Keyword(s):  
Cell Growth ◽  
Cancer Cells ◽  
Real Time ◽  
Gold Nanoparticle ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Differential Toxicity ◽  
Kinetics Of

The kinetics of toxicity of doxorubicin (Dox) and gold nanoparticle-conjugated doxorubicin (Au-Dox) were investigated in cultured B16 melanoma cells and cardiomyocytes using real-time cell-growth imaging.

scholarly journals Using real-time impedance-based assays to monitor the effects of fibroblast-derived media on the adhesion, proliferation, migration and invasion of colon cancer cells

2014 ◽  
Vol 34 (4) ◽  
Author(s):  
Catríona M. Dowling ◽  
Carmen Herranz Ors ◽  
Patrick A. Kiely
Keyword(s):  
Colon Cancer ◽  
Cell Adhesion ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Real Time ◽  
Colon Cancer Cell ◽  
Migration And Invasion ◽  
Colon Cancer Cells ◽  
Cancer Cell Adhesion ◽  
Kinetics Of

Here, we use an RTCA platform to assess the kinetics of colon cancer cell adhesion, proliferation, migration and invasion in response to media derived from fibroblasts. This work highlights the importance of considering the stromal environment in cancer treatment.

scholarly journals CDX2 and Reg IV expression and correlation in gastric cancer

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dandan Chai ◽  
Huifen Du ◽  
Kesheng Li ◽  
Xueliang Zhang ◽  
Xiaoqin Li ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Migration ◽  
Cancer Cells ◽  
Real Time ◽  
Real Time Pcr ◽  
Ectopic Expression ◽  
Rank Correlation ◽  
Migration And Invasion ◽  
Gastric Cancer Cells ◽  
Cell Migration And Invasion

Abstract Background Ectopic expression of CDX2 is associated with the development and progression of gastric cancer. Previous studies showed that CDX2 may be an upstream regulator of Reg IV expression in gastric cancer, and our previous report showed that Reg IV upregulated SOX9 expression and enhanced cell migration and invasion in gastric cancer cells. However, the regulatory roles of CDX2 have not been clarified in gastric cancer, and the correlation between CDX2 and Reg IV requires further study. Methods CDX2 and Reg IV were examined in gastric cancer specimens and paired adjacent tissues via real-time PCR and immunohistochemistry (IHC). The association between CDX2 and Reg IV was assessed using the χ2-test and Spearman’s rank correlation. To verify their relationship, knockdown and exogenous expression of CDX2 or Reg IV were performed in AGS and MKN-45 gastric cancer cells, and their expression was subsequently analyzed via a real-time PCR and western blotting. Wound-healing and Transwell assays were used to examine migration and invasion in AGS and MKN-45 cells following CDX2 silencing or overexpression. Results A positive correlation was observed between CDX2 and Reg IV expression at the mRNA and protein levels in gastric cancer tissues. CDX2 silencing significantly downregulated Reg IV expression, and CDX2 overexpression significantly upregulated Reg IV expression in AGS and MKN-45 cells. Neither Reg IV silencing nor overexpression had any effect on CDX2 protein expression in AGS or MKN-45 cells, even though both affected the expression of CDX2 mRNA. Functionally, CDX2 silencing significantly inhibited cell migration and invasion, and CDX2 overexpression significantly promoted cell migration and invasion in AGS and MKN-45 cells. Conclusions Our findings demonstrate that CDX2 expression was positively correlated with that of Reg IV in gastric cancer, and CDX2 promoted cell migration and invasion through upregulation of Reg IV expression in AGS and MKN-45 cells.

A nanostructured microfluidic device for plasmonic-assisted electrochemical detection of hydrogen peroxide released from cancer cells

Nanoscale ◽  
2021 ◽  
Author(s):  
Carolina del Real Mata ◽  
Roozbeh Siavash Moakhar ◽  
Sayed Iman Isaac Hosseini ◽  
Mahsa Jalali ◽  
Sara Mahshid
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Real Time ◽  
Electrochemical Detection ◽  
Microfluidic Device ◽  
Cancer Biomarker ◽  
Cancer Diagnostics ◽  
Liquid Biopsies ◽  
Non Invasive

Non-invasive liquid biopsies offer hope for a rapid, risk-free, real-time glimpse into cancer diagnostics. Recently, hydrogen peroxide (H2O2) is identified as a cancer biomarker due to continued release from cancer...

Kinetics of interaction of HIV fusion protein (gp41) with lipid membranes studied by real-time AFM imaging

2010 ◽  
Vol 110 (6) ◽  
pp. 694-700 ◽  
Author(s):  
Arkady Bitler ◽  
Naama Lev ◽  
Yael Fridmann-Sirkis ◽  
Lior Blank ◽  
Sidney R. Cohen ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Real Time ◽  
Lipid Membranes ◽  
Afm Imaging ◽  
Protein Gp41 ◽  
Kinetics Of
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