scholarly journals Topical anti-inflammatory activity of n-hexane extract of santalum album linn leaves on rat ear oedema induced by croton oil

2019 ◽  
Vol 1321 ◽  
pp. 022033 ◽  
Author(s):  
N M Puspawati ◽  
W S Rita
Keyword(s):  
Hexane Extract ◽  
Inflammatory Activity ◽  
Santalum Album ◽  
Croton Oil ◽  
Anti Inflammatory ◽  
Ear Oedema

scholarly journals Anti-Inflammatory Activity of Cnidoscolus aconitifolius (Mill.) Ethyl Acetate Extract on Croton Oil-Induced Mouse Ear Edema

2021 ◽  
Vol 11 (20) ◽  
pp. 9697
Author(s):  
Eduardo Padilla-Camberos ◽  
Omar Ricardo Torres-Gonzalez ◽  
Ivan Moises Sanchez-Hernandez ◽  
Nestor Emmanuel Diaz-Martinez ◽  
Oscar Rene Hernandez-Perez ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inflammatory Activity ◽  
Therapeutic Effects ◽  
Ear Edema ◽  
Croton Oil ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema

Nowadays, there is a growing interest in the development of medicinal plant-based therapies to diminish the ravages of the inflammatory process related to diseases and tissue damage. Most therapeutic effects of these traditional medicinal plants are owed to their phenolic and antioxidant properties. C. aconitifolius is a traditional medicinal plant in Mexico. Previous characterization reports have stated its high nutritional and antioxidant components. The present study aimed to better understand the biological activity of C. aconitifolius in inflammation response. We developed an ethyl acetate extract of this plant to evaluate its anti-inflammatory capacity and its flavonoid content. The topical anti-inflammatory effect of the ethyl acetate extract of C. aconitifolius was determined by the croton oil-induced mouse ear edema test, while flavonoid detection and concentration were determined by thin layer chromatography and the aluminum chloride colorimetric assay, respectively. Topical application of the extract showed significant inhibition of the induced-ear edema (23.52 and 49.41% for 25 and 50 mg/kg dose, respectively). The extract also exhibited the presence of flavonoids. The finding of the anti-inflammatory activity exerted by the C. aconitifolius and the identification of its active principles may suggest and support its use for inflammation treatment.

scholarly journals In vitro antioxidant anti inflammatory and cytotoxicity activities from Hexane extract of Bryonopsis laciniosa fruits.

2018 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Sanjeevkumar C.B ◽  
Ramesh L Londonkar ◽  
Umesh Madire Kattegouda
Keyword(s):  
Cell Line ◽  
Potential Application ◽  
Protein Denaturation ◽  
Cancer Cell Line ◽  
Hexane Extract ◽  
Inflammatory Activity ◽  
Cytotoxicity Activity ◽  
Anti Inflammatory ◽  
Mcf 7

<p><em>Bryonopsis laciniosa </em>also known as “Shivlingi” annual climber with bright red fruits and is reported to be highly medicinal in India<em>. </em>As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study, Hexane extract of <em>B. laciniosa </em>fruits were used to evaluate <em>in</em> <em>vitro</em> anti inflammatory, antioxidant and Cytotoxicity (towards MCF-7 cell line) activities.<em> In vitro</em> anti inflammatory activity by inhibition of protein denaturation, antioxidant assays like DPPH, ABTS, H<sub>2</sub>O<sub>2</sub> and FRAP were used to measure the antioxidant capacity of the hexane extracts and cytotoxicity activity using MCF-7 breast cancer cell line. Hexane extract showed the effective antioxidant activity in all assays compared to ascorbic acid and BHT. The results for<em> In vitro</em> anti inflammatory activity of hexane extract and Dichlofenac drug were equivalent, hexane extract showed promising activity for inhibition of protein denaturation assay. The cytotoxicity activity from hexane extract was noticeable against MCF-7 cell line. The overall results show potential application of <em>Bryonopsis laciniosa </em>fruits suggesting their potential application as a health-promoting functional ingredient or natural preservative in foods.</p>

scholarly journals Screening for anti-inflammatory activity of extract and fractions of Morinda lucida stem bark and detection of bioactive secondary metabolite

2021 ◽  
Vol 16 (1) ◽  
pp. 031-039
Author(s):  
Chukwubuikem C. Okolo ◽  
Nancy A. Mbachu ◽  
Ogechi O. Anyanwu ◽  
Kenneth G. Ngwoke ◽  
Festus B.C. Okoye
Keyword(s):  
Stem Bark ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Paw Oedema ◽  
Dependent Inhibition ◽  
Egg Albumen ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Ear Oedema ◽  
Rat Paw Oedema

Morinda lucida Benth (Rubiaceae) is a versatile plant used in traditional medicine for the treatment of a variety of ailments and the claims of its efficacy are particularly remarkable in the treatment of infections and immuno-inflammatory disorders. This study evaluates the anti-inflammatory properties of methanolic stem bark extract and fractions of M. lucida and also identifies the phytochemicals responsible for its anti-inflammatory activity. The crude extract was subjected to liquid- liquid partitioning successively with n- hexane, ethylacetate, butanol and water. High performance liquid chromatography (HPLC) of the four fractions and Vacuum Liquid Chromatography fraction (VLC) of the promising fraction was evaluated. The effect of the fractions on egg albumen induced rat paw oedema were also evaluated. Anti-inflammatory activity of the fractions was further screened using xylene induce ear oedema models and human red blood cell membrane stabilization test. Ulcerogenic test on the normal stomach mucosa was also evaluated. The result of the egg albumen induced rat paw oedema showed that the butanol fractions maximally inhibited egg albumen induced effect at 400 mg/kg (70%) and 200mg/kg (67.5%) after 180 minutes compared to the positive control, ibuprofen (20mg/kg) with 100% inhibition after 180 minutes. The result of the xylene induced ear oedema showed that the inhibition produced by 100 µg/ear of the Butanol fraction (BF) was 56.67 % and was greater than inhibition produced by 200 µg/ear of ibuprofen (38.89 %). HPLC analysis of the fractions revealed the following phytocompounds; Cytreo- a-pyrone, Cytosporin- J and Waol A. Ulcerogenic test was negative at the doses of 200 mg/kg and 400 mg/kg of the fractions when compare with the indomethacin (positive control) at dose of 50 mg/kg. Human red blood cell membrane stabilization assay showed that BF-VLC 2 (Dichloromethane: methanol (8:2) VLC of Butanol fraction) exhibited concentration dependent inhibition of heat-induced haemolysis while other extract showed a non- concentration dependent inhibition of haemolysis when compared to the standard, ibuprofen. These findings suggest that the stem bark of M. lucida possess promising anti-inflammatory phytocompounds which justify its use in ethno-medicine.

Anti-inflammatory activity of Aloe vera against a spectrum of irritants

1989 ◽  
Vol 79 (6) ◽  
pp. 263-276 ◽  
Author(s):  
RH Davis ◽  
MG Leitner ◽  
JM Russo ◽  
ME Byrne
Keyword(s):  
Broad Spectrum ◽  
Aloe Vera ◽  
Time Dependent ◽  
Inflammatory Activity ◽  
Croton Oil ◽  
Oral Activity ◽  
Anti Inflammatory

The authors have evaluated the spectrum of anti-inflammatory activity of A. vera in a number of models of inflammation in the hind paw of the experimental rat induced by kaolin, carrageenan, albumin, dextran, gelatin, and mustard. Croton oil was used in a topical model of inflammation to determine the oral activity and time-dependent dosing of A. vera. The authors found that A. vera was active in all models of inflammation. Of the various irritants tested, A. vera was especially active against gelatin-induced and kaolin-induced edema and, in contrast, had minimal activity when tested against dextran-induced edema. Oral activity of A. vera was demonstrated to be dependent on the presence of anthraquinones. The various irritant-induced edema models provided a broad spectrum of anti-inflammatory activity for A. vera.

Isolation of a stimulatory system in an Aloe extract

1991 ◽  
Vol 81 (9) ◽  
pp. 473-478 ◽  
Author(s):  
RH Davis ◽  
WL Parker ◽  
RT Samson ◽  
DP Murdoch
Keyword(s):  
Wound Healing ◽  
Lower Extremity ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Supernatant Fraction ◽  
Croton Oil ◽  
Precipitate Fraction ◽  
Anti Inflammatory ◽  
Wound Healing Activity

The authors' previous work on a 50% ethanol extract of Aloe vera was done to evaluate anti-inflammatory activity using the croton oil-induced ear swelling assay. The anti-inflammatory activity was found in the supernatant fraction. The supernatant fraction decreased inflammation, when applied topically, by 29.2%, and the precipitate decreased inflammation by 12.1%. However, in the present work, the precipitate fraction decreased the wound diameter by an average of 47.1% (stimulatory system). Little or no wound healing activity was found in the supernatant. Aloe vera appears to act as a modulatory system toward wounds and inflammation and is a potentially valuable tool for managing lower extremity conditions.

scholarly journals Antinociceptive and Anti-Inflammatory Activities of the Ethanolic Extract fromSynadenium umbellatumPax. (Euphorbiaceae) Leaves and Its Fractions

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Rodrigo Borges ◽  
Marcus Vinícius Mariano Nascimento ◽  
Adryano Augustto Valladão de Carvalho ◽  
Marize Campos Valadares ◽  
José Realino de Paula ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Antinociceptive Activity ◽  
Opioid System ◽  
Tail Flick ◽  
Tail Flick Test ◽  
Ear Edema ◽  
Croton Oil ◽  
Anti Inflammatory

Synadenium umbellatumPax., popularly known in Brazil as “cola-nota,” “avelós,” “cancerola,” and “milagrosa”, is a plant species used in folk medicine for the treatment of inflammation, pain, and several diseases. This study aimed to investigate the antinociceptive and anti-inflammatory activities of the ethanolic extract fromSynadenium umbellatumPax. leaves (EES) and its hexane (HF), chloroform (CF), and methanol/water (MF) fractions using the acetic acid-induced abdominal writhing test, formalin-induced paw licking test, tail flick test, croton oil-induced ear edema test, and carrageenan-induced peritonitis test. EES and MF reduced the number of acetic acid-induced abdominal writhes, while CF and HF did not. EES effect on acetic acid-induced abdominal writhing was reversed with a pretreatment with naloxone. EES reduced licking time in both phases of the formalin-induced paw licking test, but did not prolong the latency in the tail flick test. These results show that EES presented antinociceptive activity, probably involving the opioid system, anti-inflammatory activity in the croton oil-induced ear edema test, and leukocyte migration into the intraperitoneal cavity. MF also presented anti-inflammatory activity in the croton oil-induced ear edema test. In conclusion, EES and MF have antinociceptive activity involving the opioid system and anti-inflammatory activity.

scholarly journals Cecropia pachystachya: A Species with ExpressiveIn VivoTopical Anti-Inflammatory andIn VitroAntioxidant Effects

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Natália Ramos Pacheco ◽  
Nícolas de Castro Campos Pinto ◽  
Josiane Mello da Silva ◽  
Renata de Freitas Mendes ◽  
Juliana de Carvalho da Costa ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Inflammatory Activity ◽  
Histopathological Analysis ◽  
Croton Oil ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Cecropia pachystachyais a species traditionally used in Brazil to treat inflammation. This work aims to evaluate the topical anti-inflammatory and antioxidant activities of the methanolic extract ofC. pachystachya(CPM) and to perform its chemical fingerprint by HPLC-DAD. The topical anti-inflammatory activity was evaluated using the mouse models of acute ear inflammation induced by croton oil, arachidonic acid, capsaicin, EPP, phenol, and chronic inflammation induced by multiple application of croton oil. Thein vitroantioxidant effect of CPM was investigated using DPPH, reducing power,β-carotene bleaching, and TBARS assays. HPLC analysis was performed to quantify the antioxidant phenolics orientin, isoorientin, and chlorogenic acid previously identified in CPM. CPM exhibited significant anti-inflammatory effect in the acute models, in some cases comparable to the reference drugs. Histopathological analysis showed a moderate chronic skin anti-inflammatory effect with decrease in vasodilation, edema, cell infiltration, and epidermal hyperproliferation. It also showed strongin vitroantioxidant activity. The contents of orientin, isoorientin, and chlorogenic acid were 66.5 ± 1.8, 118.8 ± 0.7, and 5.4 ± 0.2 µg/mg extract, respectively. The topical anti-inflammatory activity of CPM could be based on its antioxidant properties, although other effects are probably involved, including COX inhibition and other mechanisms.

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