AbstractWe propose a new scheme for creating three-dimensional Skyrmions in a ferromagnetic spin-1 Bose-Einstein condensate by manipulating a multipole magnetic field and a pair of counter-propagating laser beams. The result shows that a three-dimensional Skyrmion with topological number Q = 2 can be created by a sextupole magnetic field and the laser beams. Meanwhile, the vortex ring and knot structure in the Skyrmion are found. The topological number can be calculated analytically in our model, which implies that the method can be extended to create Skyrmions with arbitrary topological number. As the examples, three-dimensional Skyrmions with Q = 3, 4 are also demonstrated and are distinguishable by the density distributions with a specific quantization axis. These topological objects have the potential to be realized in ferromagnetic spin-1 Bose-Einstein condensates experimentally.