scholarly journals Amplification rate of matK and rbcL genes in three types of durian

2021 ◽  
Vol 886 (1) ◽  
pp. 012011
Author(s):  
Y F Cahyaningsih ◽  
Sukartini ◽  
A Sutanto ◽  
P J Santoso ◽  
M A Arsyad ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Plant Species ◽  
Genetic Relationship ◽  
Genetic Relationships ◽  
Chloroplast Genes ◽  
Rbcl Sequence ◽  
Durio Zibethinus ◽  
Amplification Rate ◽  
Plant Sequence

Abstract Durian is a tropic fruit having numerous variations on its fruits. Its variations are not only in its shape but also in its aril fruit, aril color, flavor, and aril thickness. In addition to its fruit variations, the genus Durio also has many species which quite hard to distinguish morphologically, except during flowering and fruiting times. This study aimed to determine the genetic relationship among Durian, Pelangi Atuturi Variety Durian, Durio graveolent, and Durio zibetinus based on chloroplast genes (RbcL and matK genes). The primers were previously designed for amplifying matK and rbcL genes based on the Durio zibethinus sequence. Both genes were used because of having great competence to describe genetic relationships between plant species. The rbcL primer could amplify all evaluated samples. Meanwhile, matK primer generated a smeared band in Durian Pelangi; thus, we did not obtain any sequence of this plant. Sequence analysis showed no variation of rbcL sequence in these evaluated species. A similar result was also observed on D. zibethinus and D. graveolent. Overall, both genes could not describe the genetic relationship among the evaluated durians, and they were grouped in the same cluster in phylogenetic.

Comparative characteristics of clinical and genetic relationships pathology amount of amniotic fluid and congenital malformations of the fetus

2015 ◽  
Vol 21 (31) ◽  
pp. 51-55
Author(s):  
Каркусова ◽  
Alla Karkusova
Keyword(s):  
Comparative Analysis ◽  
Amniotic Fluid ◽  
Genetic Relationship ◽  
Fetal Development ◽  
Congenital Malformations ◽  
Genetic Relationships ◽  
Case Histories ◽  
History Of ◽  
Genetic Consultation

A comparative analysis of clinical and genetic relationship pathology of amniotic fluid and congenital malformations during the study 12150 case histories of women aged 16 to 45 years. To identify the disease heritability amount of amniotic fluid and to establish relationships with congenital fetal development floggings calculation of direct and indirect correlations between them. Was used the estimation of the genetic community pathological content of the amniotic fluid and malformations, which amounted to 27.9% at oligohydramnios, at polyhydramnios - 25.8%, which allows women who have a history of pregnancy with abnormal content of amniotic fluid to send to the institution of medico-genetic consultation in order to get the recommendations for planning future pregnancies.

scholarly journals Genomic Profiling of F1 Hybrids of Durian (Durio zibethinus) Revealed by RAPD-PCR

2016 ◽  
Vol 24 (2) ◽  
pp. 69-76 ◽  
Author(s):  
Riry Prihatini ◽  
Farihul Ihsan ◽  
Ni Luh Putu Indriyani
Keyword(s):  
Molecular Analysis ◽  
Genetic Relationships ◽  
Female Parent ◽  
Similarity Coefficient ◽  
F1 Hybrids ◽  
Genomic Profiling ◽  
Pca Analysis ◽  
Genetic Characteristics ◽  
Durio Zibethinus ◽  
Rapd Pcr

Abstract The molecular analysis of 32 durian F1 hybrids, resulted from crossing of the Arp 8990 (female parent) and ‘Otong’ (male parent), was conducted in order to determine the genetic characteristics of hybrids and parents, as it would be followed/evidenced by the variability of traits produced from the cross breeding. The RAPD analyses of 14 primers resulted in 114 scoring bands, 112 (98.2%) of them were polymorphic, with 4 to 11 bands amplified per primer. The electrophoresis gel of the PCR results revealed that some hybrids produced different band patterns compared to the parents; this indicated the crossing between parents’ alleles and trait combinations from both the parents. The Dice-Sorensen similarity coefficient demonstrated that most of the hybrids had distant genetic similarities with both parents, which were ranged from 0.141 [71B(4) and 72B(15)] to 0.776 [71B(15) and 48B(1)]. The UPGMA method was used to construct the dendrogram, which grouped the hybrids in five clusters with distinct genetic relationships and was confirmed with the PCA analysis. This result implied that above crossing produced hybrids having characters different from the parents.

scholarly journals Genetic Analysis of Tropical Orchid Hybrids (Dendrobium) with Fluorescence Amplified Fragment-length Polymorphism (AFLP)

2003 ◽  
Vol 128 (5) ◽  
pp. 731-735 ◽  
Author(s):  
N. Xiang ◽  
Y. Hong ◽  
L.T. Lam-Chan
Keyword(s):  
Length Polymorphism ◽  
Genetic Relationship ◽  
Fragment Length Polymorphism ◽  
Genetic Relationships ◽  
Aflp Analysis ◽  
Tropical Orchid ◽  
Trade Names ◽  
Relationship Of ◽  
Intensive Breeding ◽  
Different Parts

Intensive breeding activities of tropical orchids have given rise to many hybrids, among which genetic relationships are difficult to evaluate due to free interbreeding of different species in the same genus or even from different genera, the use of hybrids for further breeding, use of abbreviated or trade names and sometimes intentional non-disclosure of parentage for commercial considerations. We have subjected 43 popular commercial Dendrobium hybrids to fluorescence amplified length polymorphism (AFLP) analysis and their genetic relationship was estimated. The hybrids bearing flowers of similar shapes and colors were clustered into five groups. Each hybrid tested had a distinct AFLP fingerprint profile except the tissue culture mutants. Sibling hybrids were closely clustered (with genetic distance <0.09) followed by those sharing one parent. These results suggest that AFLP fingerprint profiling gives accurate and objective estimation of genetic relationship of the Dendrobium hybrids tested. Our study also found that the AFLP fingerprint profiles were uniform in different parts of tested plants, stable among individuals in vegetatively propagated populations throughout different growth periods. We conclude that AFLP fingerprint profiling has the potential to be an integral part of current new plant varieties protection sytems.

scholarly journals DNA Fingerprinting to Identify Nine Anthurium Pot Plant Cultivars and Examine Their Genetic Relationship

HortScience ◽  
2001 ◽  
Vol 36 (4) ◽  
pp. 758-760 ◽  
Author(s):  
D.G. Ranamukhaarachchi ◽  
R.J. Henny ◽  
C.L. Guy ◽  
Q.B. Li
Keyword(s):  
Genetic Relationship ◽  
Rapd Markers ◽  
Parental Species ◽  
Genetic Relationships ◽  
Dna Fingerprints ◽  
Randomly Amplified Polymorphic Dna ◽  
Arbitrary Primers ◽  
D Values ◽  
High Degree ◽  
Pot Plant

Randomly amplified polymorphic DNA (RAPD) markers were utilized to determine the genetic relationships of nine morphologically similar pot plant cultivars of Anthurium sp. by developing DNA fingerprints (DFP). Of 25 arbitrary primers screened, nine generated DFPs that were used in computing the genetic distance (d) and similarity coefficient (C) values. All cultivars tested exhibited a high degree of genetic similarity. `Lady Ann' and `Lady Beth' possessed the closest relationship with d and C values of 0.06 and 0.98, respectively. The next closest genetic relationship was between `Red Hot' and `Southern Blush' (d = 0.33, C = 0.89). These two cultivars exhibited a more distant relationship to the other seven cultivars as indicated by higher `d' values. However, this study showed that the nine Anthurium cultivars examined were genetically closely related. These cultivars share specific DNA bands with three possible parental species (A. andraeanum Linden ex Andre, A. antioquens L., and A. amnicola Dressler) included in this study, which may indicate similarities in their pedigree. This study shows that RAPDs can be a useful tool to distinguish Anthurium pot plant cultivars as well as identify their genetic relationships.

scholarly journals Genetic Relationship of Transcriptional Factor Genes in the Regulation of Cells Death in Arabidopsis: A Review

2021 ◽  
Author(s):  
A.J. Khaskheli ◽  
L. Zhang ◽  
M.I. Khaskheli ◽  
A.A. Khaskheli ◽  
L.H. Qing
Keyword(s):  
Cell Death ◽  
Transcription Factors ◽  
Genetic Relationship ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Genetic Relationships ◽  
Transcriptional Factor ◽  
Environmental Strain ◽  
Relationship Of ◽  
Cells Death

The leaf yellowing is the first visible sign of senescence, which starts at the margins of the leaf and progresses to the blade. Although, transcriptional factor family genes generally encode meticulous regulators which perform a range of functions in turns regulating the physiological and developmental mechanism of plant stature. However, the genetic relationship of TFs genes in regulating the cell death of Arabidopsis is well not understood to date. TFs family in a plant regulates various developmental and stress responses in underline pathways. In our review we observed the genetic relationship of TFs genes in regulations of cell death in Arabidopsis. Given that, programmed cell death (PCD) being an active process that includes the expression of hundreds of genes. It is speculated that many TFs are involved in the core elements of the regulatory network. There are only a few factors that are being demonstrated in involving the regulation of cell death, by evaluating the leaf senescence appearances of knocking of mutants and by identifying downstream target genes. In this review, we have focused on the manifold roles of TFs during genetic relationships and the regulation of cell death in Arabidopsis. We also deliberated how the transcription factors family gene regulates the cells’ death by different hormonal stress, environmental strain and their role in retrograde signaling. For deep understanding of regulatory molecular mechanisms of cell death in the plant, future research may be hypothesized to collect appropriate evidence and a detailed study may be implemented on the upstream pathway with a specific targeted gene that recognizes the stress signals involved in cell death in plants. Also, crosstalk between mitochondria and chloroplast is mainly being focused to better understand the regulations of cell death in plants. Present review concludes that regulating the cell death of Arabidopsis is very important for meeting future global food needs, crop yields. Overexpression of ERF transcription factors genes relating cell death of Arabidopsis confers broad-spectrum resistance to pathogens and other abiotic stresses and can also make transgenic plants resistant to drought, salinity and freezing.

scholarly journals DNA Sequence Analysis of Microsatellite Markers Enhances Their Efficiency for Germplasm Management in an Italian Olive Collection

2006 ◽  
Vol 131 (3) ◽  
pp. 352-359 ◽  
Author(s):  
Innocenzo Muzzalupo ◽  
Nicola Lombardo ◽  
Aldo Musacchio ◽  
Maria Elena Noce ◽  
Giuseppe Pellegrino ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Microsatellite Markers ◽  
Dna Sequence ◽  
Genetic Relationships ◽  
Germplasm Collection ◽  
Electrophoretic Analysis ◽  
Dna Sequence Analysis ◽  
Germplasm Management ◽  
Olive Cultivars ◽  
High Level

Genetic diversity studies using microsatelite analysis were carried out in a set of 39 accessions of Olea europaea L., corresponding to the majority of the regional autochthon germplasm in Apulia. Samples of olive leaves were harvested from plants growing in the olive germplasm collection of the Consiglio per la Ricerca e Sperimentazione in Agricoltura (C.R.A.) - Istituto Sperimentale per l'Olivicoltura at Rende in Cosenza Italy. Herein, we evaluated the extent to which microsatellite analysis using electrophoresis was capable of identifying traditional olive cultivars. In addition, the DNA sequence of all amplicons was determined and the number of repeat units was established for each sample. Using five loci, electrophoretic analysis identified 24 genotype profiles, while DNA sequence analysis detected 28 different genotype profiles, identifying 54% of cultivars. The remaining 46% were composed of seven different accession groups containing genetically indistinguishable cultivars, which are presumably synonyms. This study demonstrates the utility of microsatellite markers for management of olive germplasm and points out the high level of polymorphisms in microsatellite repeats when coupled with DNA sequence analysis. The establishment of genetic relationships among cultivars in the Apulian germplasm collection allows for the construction of a molecular database that can be used to establish the genetic relationships between known and unknown cultivars.

scholarly journals Estimation of Genetic Variation in Grapes Based on RAPD and Isozyme Analysis

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 513E-513
Author(s):  
J. Lu ◽  
Z. Liu ◽  
Y. Zheng
Keyword(s):  
Genetic Variation ◽  
Genetic Relationship ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Isozyme Analysis ◽  
Similarity Coefficients ◽  
Taxonomic Information ◽  
Geographic Origins ◽  
Asiatic Species ◽  
Rapd Method

Genetic relationships among 42 grape accession of at least 15 species were estimated and compared using RAPD and isozyme techniques. These accessions were either hybrids or wild collections of the Asiatic species, the American species, the European grape (V. vinifera), and muscadine grape (V. rotundifolia). A total of 196 RAPD fragments were generated from twenty 10-mer primers. The pairwise similarities among the accession ranged from 0.46 to 0.94. A dendrogram was generated based on the RAPD similarity coefficients. Species/accessions were basically grouped together in accordance with their geographic origins. The similarities and dendrogram resulted from the RAPD analysis were consistent with the ones generated from the isozyme data, and also consistent with the known taxonomic information. This result suggest that the RAPD method, like isozyme, is an useful tool for studying grape genetic relationship/diversity and origination.

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