scholarly journals Characterization and Evaluation The Biological Activity of Prepared Nano-Gentamicin Nanoparticles

2021 ◽  
Vol 910 (1) ◽  
pp. 012078
Author(s):  
Rana A. Kamal ◽  
Quraish A. Kahdhum ◽  
Awatif I. Mohammed ◽  
Ahmed J. Essa ◽  
Maan A. Abd elhamid ◽  
...  
Keyword(s):  
Biological Activity ◽  
Antibacterial Effect ◽  
Sol Gel ◽  
Veterinary Drug ◽  
Homogeneous Distribution ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Force Microscopy ◽  
Average Size

Abstract Nanotechnology is a capable approach to enhance the activity of common antimicrobial agent mainly used in human and veterinary drug. Gentamycin is a broad-spectrum antibacterial against Gram-positive and Gram-negative bacteria. The study aims to determine the characterization and the biological activity of gentamicin in the Nano-form prepared by Sol-gel application using an ultrasound device by aqueous solution at temperature (80)0C. Nano gentamycin examined by Scanning electron microscopy (SEM), transmission (TEM), atomic force microscopy (AFM) and antibacterial effect of both Nano and stander gentamycin were analyzed. The results showed that the average size of gentamycin nanoparticles was 68.51 nanometers with homogeneous distribution. The EDX showed large number of elements present in the Nano solution. The antibacterial effect of Nano gentamycin took a wide inhibition range of Gram-positive and Gram-negative bacteria compared to the standard substance, where the inhibition diameter for E.coli was 29 mm, and for St. epidermidis was 27 mm. The MIC of gentamicin nanostructures was the concentration 0.00125 mg/ml.

scholarly journals Evaluation the Biological Activity of Nano- Gentamicin Prepared and Characterization of Nanoparticles

2021 ◽  
Vol 8 (2) ◽  
pp. 84-90
Author(s):  
Rana Kamal ◽  
Quraish A. Kahdhum ◽  
Awatif I. Mohammed ◽  
Ahmed J. Essa ◽  
Maan A. Abd Elhamid ◽  
...  
Keyword(s):  
Biological Activity ◽  
Antibacterial Effect ◽  
Sol Gel ◽  
Homogeneous Distribution ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Average Size

Gentamycin is a broad-spectrum antibacterial against Gram-positive and Gram-negative bacteria. Gentamycin Sulfate is a white powder that is freely soluble in water and insoluble in alcohol, acetone, chloroform and ether. The study aims to determine the characterization and the biological activity of gentamicin in the Nano-form prepared by Sol-gel application using an ultrasound device by aqueous solution at temperature of 80°. Nano gentamycin examined by Scanning electron microscopy (SEM), transmission (TEM) and the antibacterial effect test of both Nano and stander gentamycin were analyzed. The results showing that the average size of gentamycin nanoparticles is 68.51 nanometers with homogeneous distribution. The antibacterial effect of Nano gentamycin take a wide inhibition range of Gram-positive and Gram-negative bacteria compared to the standard substance, the inhibition diameters are for E. coli (29 mm) and for St. epidermidis (27 mm). In the other side the inhibitory ability for standard gentamicin for St. epidrmidis and E. coli were 18 mm and 20 mm, respectively.

scholarly journals Long-distance electron transfer in a filamentous Gram-positive bacterium

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yonggang Yang ◽  
Zegao Wang ◽  
Cuifen Gan ◽  
Lasse Hyldgaard Klausen ◽  
Robin Bonné ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Microbial Fuel Cells ◽  
Extracellular Electron Transfer ◽  
Gram Negative Bacteria ◽  
Positive Bacterium ◽  
Long Distance ◽  
Gram Positive ◽  
Gram Negative ◽  
Force Microscopy ◽  
Cell Envelopes

AbstractLong-distance extracellular electron transfer has been observed in Gram-negative bacteria and plays roles in both natural and engineering processes. The electron transfer can be mediated by conductive protein appendages (in short unicellular bacteria such as Geobacter species) or by conductive cell envelopes (in filamentous multicellular cable bacteria). Here we show that Lysinibacillus varians GY32, a filamentous unicellular Gram-positive bacterium, is capable of bidirectional extracellular electron transfer. In microbial fuel cells, L. varians can form centimetre-range conductive cellular networks and, when grown on graphite electrodes, the cells can reach a remarkable length of 1.08 mm. Atomic force microscopy and microelectrode analyses suggest that the conductivity is linked to pili-like protein appendages. Our results show that long-distance electron transfer is not limited to Gram-negative bacteria.

scholarly journals A Xenorhabdus budapestensis entomopatogén baktérium sejtmentes fermentlevének és tisztítottfehérje-frakciójának antimikrobiális hatása néhány zoonoticus baktériumra

2015 ◽  
Vol 156 (44) ◽  
pp. 1782-1786 ◽  
Author(s):  
Erzsébet Burgettiné Böszörményi ◽  
István Barcs ◽  
Gyula Domján ◽  
Katalin Bélafiné Bakó ◽  
András Fodor ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Antibacterial Effect ◽  
Conditioned Media ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Salmonella Gallinarum ◽  
Gram Positive Bacteria ◽  
Agar Diffusion Test

Introduction: Many multi-resistant patogens appear continuously resulting in a permanent need for the development of novel antibiotics. A large number of antibiotics introduced in clinical and veterinary practices are not effective. Antibacterial peptides with unusual mode of action may represent a promising option against multi-resistant pathogens. The entomopathogenic Xenorhabdus budapestensis bacteria produce several different antimicrobial peptides compounds such as bicornutin-A and fabclavin. Aim: The aim of the authors was to evaluate the in vitro antibacterial effect of Xenorhabdus budapestensis using zoonotic patogen bacteria. Method: Cell-free conditioned media and purified peptide fractions of Xenorhabdus budapestensis were tested on Gram-positive (Rhodococcus equi, Erysipelothrix rhusiopathia, Staphylococcus aureus, Streptococcus equi, Corynebacterium pseudotuberculosis, Listeria monocytagenes) and Gram-negative bacteria (Salmonella gallinarum, Salmonella derbi, Bordatella bronchoseptica, Escherichia coli, Pasteurella multocida, Aeromonas hydrophila) using agar diffusion test on blood agar plates. Results: It was found that Xenorhabdus budapestensis bacteria produced compounds with strong and dose-dependent effects on the tested organisms. Purified peptid fraction exerted a more marked effect than cell free conditioned media. Gram-positive bacteria were more sensitive to this antibacterial effect than Gram-negative bacteria. Conclusions: Antibacterial peptide compound from Xenorhabdus budapestensis exert marked antibacterial effect on zoonotic patogen bacteria and they should be further evaluated in future for their potential use in the control or prevention of zoonoses. Orv. Hetil., 2015, 156(44), 1782–1786.

scholarly journals Two-Step Triethylamine-Based Synthesis of MgO Nanoparticles and Their Antibacterial Effect against Pathogenic Bacteria

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 410
Author(s):  
Ramiro Muñiz Diaz ◽  
Pablo Eduardo Cardoso-Avila ◽  
José Antonio Pérez Tavares ◽  
Rita Patakfalvi ◽  
Virginia Villa Cruz ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Structural Changes ◽  
Antibacterial Effect ◽  
Precipitation Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Bacterial Membranes ◽  
Force Microscopy ◽  
Mean Size ◽  
Escherichia Coli Bacteria

Magnesium oxide nanoparticles (MgO NPs) were obtained by the calcination of precursor microparticles (PM) synthesized by a novel triethylamine-based precipitation method. Scanning electron microscopy (SEM) revealed a mean size of 120 nm for the MgO NPs. The results of the characterizations for MgO NPs support the suggestion that our material has the capacity to attack, and have an antibacterial effect against, Gram-negative and Gram-positive bacteria strains. The ability of the MgO NPs to produce reactive oxygen species (ROS), such as superoxide anion radicals (O2•−) or hydrogen peroxide (H2O2), was demonstrated by the corresponding quantitative assays. The MgO antibacterial activity was evaluated against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria, with minimum inhibitory concentrations (MICs) of 250 and 500 ppm on the microdilution assays, respectively. Structural changes in the bacteria, such as membrane collapse; surface changes, such as vesicular formation; and changes in the longitudinal and horizontal sizes, as well as the circumference, were observed using atomic force microscopy (AFM). The lipidic peroxidation of the bacterial membranes was quantified, and finally, a bactericidal mechanism for the MgO NPs was also proposed.

Facile Synthesis of SiO2/CMC/Ag Hybrids Derived from Waste Biomass (Sugarcane Bagasse) Having Special Medical Application

2020 ◽  
Vol 20 (10) ◽  
pp. 6413-6421
Author(s):  
Harish Kumar ◽  
Avneesh Kumar Gehlaut ◽  
Ankur Gaur ◽  
Jin-Won Park ◽  
Sanjeev Maken
Keyword(s):  
Sugarcane Bagasse ◽  
Sol Gel ◽  
Ultra Violet ◽  
Gram Negative Bacteria ◽  
Waste Biomass ◽  
Ag Nps ◽  
Gram Positive ◽  
Gram Negative ◽  
X Ray ◽  
Gram Positive Bacteria

This research is focused on the use of sol gel technique to synthesize amorphous SiO2 hybrids derived from lab made CMC (made from sugarcane bagasse) and tetraethoxysilane (TEOS) comprising silver nanoparticles (Ag-NPs). The dominant absorption peak in the order of 425 nm confirms the presence of Ag-NPs hybrid group owing to the surface Plasmon resonance (SPR). Ag-NPs hybrid characterization of was performed by Ultra violet-visible spectra (UV-Vis), Scanning electronic microscopy (SEM), Transmission electron microscopy (TEM), Particle size analyser (PSA), Energy Dispersive X-ray spectroscopy (EDX), X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). The antibacterial action of Ag-NPs in contrast to Gram-negative bacteria (Escherichia coli) (ATCC 433) and Gram-positive bacteria (Bacillus subtilis) (ATCC 1688) was analyzed by using the method of agar disk diffusion technique. Ag-NPs hybrids extracted from lab-made CMC confirm higher adverse bacterial action through Gram-positive bacteria as well as Gram-negative bacteria related to synthetic CMC acquired from the market.

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