Short Communication: HIV Type 1 Tropism Determination in a Novel Dried Blood Spot Membrane and the Use of a Mixture of Outer Nested Polymerase Chain Reaction Primers

2014 ◽  
Vol 30 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Shirlene Lavigne ◽  
Carlos Santos ◽  
Muhammad Shoaib Arif ◽  
Alexanda Reis ◽  
Sadia Samer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Short Communication ◽  
Dried Blood Spot ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Hiv Type 1 ◽  
Polymerase Chain Reaction Primers ◽  
Blood Spot

Splicing Variability in HIV Type 1 Revealed by Quantitative RNA Polymerase Chain Reaction

1994 ◽  
Vol 10 (11) ◽  
pp. 1531-1542 ◽  
Author(s):  
MARKUS NEUMANN ◽  
JEANETTE HARRISON ◽  
MARY SALTARELLI ◽  
EMILIA HADZIYANNIS ◽  
VOLKER ERFLE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rna Polymerase ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Hiv Type 1

scholarly journals Specific Polymerase Chain Reaction Primers for the Detection of Plasmodiophora brassicae in Soil and Water

1999 ◽  
Vol 89 (5) ◽  
pp. 392-397 ◽  
Author(s):  
R. Faggian ◽  
S. R. Bulman ◽  
A. C. Lawrie ◽  
I. J. Porter
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodiophora Brassicae ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Simple Method ◽  
Soil P ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Primers ◽  
Soil And Water

The development of specific oligonucleotide primers for Plasmodiophora brassicae has led to a nested polymerase chain reaction (PCR) detection method for P. brassicae in soil and water. Initially, the PCR was used to amplify a section of the rDNA repeat. The PCR products were sequenced and the data used to design primers that were directed at the ribosomal RNA genes and internal transcribed spacer regions. Specificity was tested against more than 40 common soil organisms, host plants, and spore suspension contaminants, as well as P. brassicae isolates from around Australia and the world. Sensitivity was determined to be 0.1 fentograms (fg; 10-15 g) for pure template and as low as 1,000 spores per g of potting mix. In soil, P. brassicae was detected in all soils where the inoculum was sufficient to result in clubroot symptoms. Also outlined is a simple method of DNA extraction from soil.

Sign in / Sign up

Export Citation Format

Share Document