The Effect of Combined Pulsed Wave Low-Level Laser Therapy and Human Bone Marrow Mesenchymal Stem Cell-Conditioned Medium on Open Skin Wound Healing in Diabetic Rats

2016 ◽  
Vol 34 (8) ◽  
pp. 345-354 ◽  
Author(s):  
Ramin Pouriran ◽  
Abbas Piryaei ◽  
Ataroalsadat Mostafavinia ◽  
Sara Zandpazandi ◽  
Farzane Hendudari ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Low Level Laser Therapy ◽  
Skin Wound Healing ◽  
Level Laser ◽  
Cell Conditioned Medium

Adipose tissue derived mesenchymal stem cell (AD-MSC) promotes skin wound healing in diabetic rats

2011 ◽  
Vol 93 (2) ◽  
pp. 228-234 ◽  
Author(s):  
Mohsen Khosravi Maharlooei ◽  
Mansooreh Bagheri ◽  
Zhabiz Solhjou ◽  
Behnam Moein Jahromi ◽  
Majid Akrami ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Skin Wound Healing

scholarly journals Effect of Equal Daily Doses Achieved by Different Power Densities of Low-Level Laser Therapy at 635 nm on Open Skin Wound Healing in Normal and Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Róbert Kilík ◽  
Lucia Lakyová ◽  
Ján Sabo ◽  
Peter Kruzliak ◽  
Kamila Lacjaková ◽  
...  
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Low Level Laser Therapy ◽  
Collagen Fibres ◽  
Skin Wound Healing ◽  
Level Laser ◽  
Significant Difference ◽  
Power Densities

Background and Objective.Despite the fact that the molecular mechanism of low-level laser therapy (LLLT) is not yet known, the exploitation of phototherapy in clinical medicine and surgery is of great interest. The present study investigates the effects of LLLT on open skin wound healing in normal and diabetic rats.Materials and Methods.Four round full-thickness skin wounds on dorsum were performed in male adult nondiabetic (n=24) and diabetic (n=24) Sprague–Dawley rats. AlGaInP (635 nm, wavelength; 5 J/cm2, daily dose) was used to deliver power densities of 1, 5, and 15 mW/cm2three times daily until euthanasia.Results.PMNL infiltration was lower in the irradiated groups (15 mW/cm2). The synthesis and organisation of collagen fibres were consecutively enhanced in the 5 mW/cm2and 15 mW/cm2groups compared to the others in nondiabetic rats. In the diabetic group the only significant difference was recorded in the ratio PMNL/Ma at 15 mW/cm2. A significant difference in the number of newly formed capillaries in the irradiated group (5, 15 mW/cm2) was recorded on day six after injury compared to the control group.Conclusion.LLLT confers a protective effect against excessive inflammatory tissue response; it stimulates neovascularization and the early formation of collagen fibres.

scholarly journals Low-Level Laser Therapy with 810 nm Wavelength Improves Skin Wound Healing in Rats with Streptozotocin-Induced Diabetes

2014 ◽  
Vol 32 (4) ◽  
pp. 198-204 ◽  
Author(s):  
Ludmila Dancáková ◽  
Tomáš Vasilenko ◽  
Ivan Kováč ◽  
Katarína Jakubčová ◽  
Martin Hollý ◽  
...  
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Skin Wound ◽  
Low Level Laser Therapy ◽  
Skin Wound Healing ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Streptozotocin Induced Diabetes

Human Bone Marrow Mesenchymal Stem Cell Conditioned Medium Promotes Wound Healing in Deep Second-Degree Burns in Male Rats

2018 ◽  
Vol 206 (6) ◽  
pp. 317-329 ◽  
Author(s):  
Arefeh Aryan ◽  
Mohammad Bayat ◽  
Shahin Bonakdar ◽  
Soudabeh Taheri ◽  
Newsha Haghparast ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Conditioned Medium ◽  
Wound Closure ◽  
Burn Injury ◽  
Human Bone ◽  
Male Rats ◽  
Cell Conditioned Medium

Burn wound treatment is difficult and one of the most challenging problems in the clinic. Researchers have examined the applications of mesenchymal stem cells as a cell-based therapy for skin regeneration. But the role of human bone marrow mesenchymal stem cell conditioned medium (hBM-MSC-CM) in the treatment of burn injury remains unclear. This research aims at detecting whether hBM-MSC-CM can increase the wound healing of deep second-degree burns in male rats. In this study, 32 adult male rats per each time point were randomly divided into four groups: (1) control group, (2) sham group (DMEM), (3) common treatment group (CT), and (4) conditioned media group (CM). A 3 × 3 cm circular burn was created on the back of the rats. On postsurgical days 7, 15, and 28, the wound closure area of each wound was measured and then the skin samples were removed and analyzed using stereological methods. Wound closure area was significantly increased in the CM and CT groups on the 15th and the 28th day after burn injury compared to the control and DMEM groups. The stereological parameters and immunohistochemistry analysis of the wounds revealed significantly improved healing in the CM group compared to the control and other groups. It is concluded that these findings indicate that hBM-MSC-CM promotes skin wound healing by increasing cell proliferation, regulating collagen synthesis and collagen composition, and inducing angiogenesis at the injury site.

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