scholarly journals Acanthamoeba Keratitis: Developing a Novel Contact Lens Disinfectant Remains an Unmet Need

Re:GEN Open ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 92-94
Author(s):  
Ruqaiyyah Siddiqui ◽  
Mustafa Khamis ◽  
Taleb Ibrahim ◽  
Naveed Ahmed Khan
Keyword(s):  
Contact Lens ◽  
Unmet Need ◽  
Acanthamoeba Keratitis

scholarly journals Continuous Real-Time Motility Analysis of Acanthamoeba Reveals Sustained Movement in Absence of Nutrients

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 995
Author(s):  
Allison Campolo ◽  
Valerie Harris ◽  
Rhonda Walters ◽  
Elise Miller ◽  
Brian Patterson ◽  
...  
Keyword(s):  
Contact Lens ◽  
Contact Lenses ◽  
Axenic Culture ◽  
Acanthamoeba Keratitis ◽  
Ocular Infection ◽  
Glass Coverslip ◽  
E Coli ◽  
Total Distance ◽  
Care Systems ◽  
Different Strains

Acanthamoeba keratitis is a serious ocular infection which is challenging to treat and can lead to blindness. While this pathogen is ubiquitous and can contaminate contact lenses after contact with water, its habits remain elusive. Understanding this organism’s natural behavior will better inform us on how Acanthamoeba colonize contact lens care systems. Acanthamoeba trophozoites were allowed to adhere to either a glass coverslip or non-nutrient agar (NNA) within a flow cell with nutrients (Escherichia coli or an axenic culture medium (AC6)) or without nutrients (Ringer’s solution). Images were taken once every 24 s over 12 h and compiled, and videos were analyzed using ImageJ Trackmate software. Acanthamoeba maintained continuous movement for the entire 12 h period. ATCC 50370 had limited differences between conditions and surfaces throughout the experiment. Nutrient differences had a noticeable impact for ATCC 30461, where E. coli resulted in the highest total distance and speed during the early periods of the experiment but had the lowest total distance and speed by 12 h. The Ringer’s and AC6 conditions were the most similar between strains, while Acanthamoeba in the E. coli and NNA conditions demonstrated significant differences between strains (p < 0.05). These results indicate that quantifiable visual tracking of Acanthamoeba may be a novel and robust method for identifying the movement of Acanthamoeba in relation to contact lens care products. The present study indicates that Acanthamoeba can undertake sustained movement for at least 12 h with and without nutrients, on both rough and smooth surfaces, and that different strains have divergent behavior.

scholarly journals Results of case-control studies support the association between contact lens use and Acanthamoeba keratitis

2013 ◽  
pp. 991 ◽  
Author(s):  
Elena Pacella ◽  
La Torre ◽  
De Giusti ◽  
Brillante ◽  
Lombardi ◽  
...  
Keyword(s):  
Contact Lens ◽  
Acanthamoeba Keratitis ◽  
Case Control ◽  
Case Control Studies

scholarly journals Continuous 24-hour measurement of intraocular pressure in millimeters of mercury (mmHg) using a novel contact lens sensor: Comparison with pneumatonometry

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248211
Author(s):  
Kevin Gillmann ◽  
Robert Wasilewicz ◽  
Kirsten Hoskens ◽  
Sonja Simon-Zoula ◽  
Kaweh Mansouri
Keyword(s):  
Contact Lens ◽  
Open Angle Glaucoma ◽  
Unmet Need ◽  
The Novel ◽  
Open Label ◽  
Fellow Eye ◽  
Corneal Erosion ◽  
Removal Time ◽  
Severe Intensity ◽  
Contact Lens Sensor

Purpose To address the unmet need of continuous IOP monitoring, a Pressure-Measuring Contact Lens (PMCL) was developed to measure IOP in millimeters of mercury (mmHg) continuously over 24 hours. The present study assessed the reliability of the novel PMCL. Methods In this prospective open-label clinical study, healthy and open-angle glaucoma (OAG) subjects were fitted with the PMCL, and pneumatonometry was performed on study eyes (in absence of the PMCL) and on fellow eyes before, during, and after provocative tests. The primary outcome measures were (1) mean IOP difference between same-eye measurements, and (2) percentage of timepoints at which IOP measured by the PMCL was within 5 mmHg of that measured by pneumatonometry in the fellow eye. Results Eight subjects were analysed (4 healthy, 4 OAG). The average difference in successive IOP measurements made by pneumatonometry and with the PMCL was 2.0±4.3mmHg at placement-time, and 6.5±15.2mmHg at removal time. During water drinking test, a significant increase in IOP was detected both by PMCL in the study eye (2.4±2.5mmHg, p = 0.03) and by pneumatonometry in the fellow eye (1.9±1.9mmHg, p = 0.02). Over the 24-hour recording, 88.0% of IOP variations measured by the PMCL were within 5mmHg of that measured with the pneumatonometer in the fellow eye. A transient corneal erosion of severe intensity was observed following removal of the PMCL on one single eye, and may have affected measurement accuracy in that eye. Conclusions This study is a proof-of-concept for this novel PMCL, and its results are encouraging, with a fair accuracy in IOP values measurement and good sensitivity to subtle IOP variations.

Awareness of Contact Lens Related Ocular Complications among Opticians of Lahore

2021 ◽  
Vol 37 (4) ◽  
Author(s):  
Ahmed Sohail ◽  
Zain Ul Abideen ◽  
Fatima Zahid ◽  
Wahid Bakhsh
Keyword(s):  
Contact Lens ◽  
Contact Lenses ◽  
Corneal Opacity ◽  
Acanthamoeba Keratitis ◽  
Bacterial Keratitis ◽  
Age Group ◽  
Cross Sectional Survey ◽  
Ocular Complications ◽  
Cross Sectional ◽  
One Year

Purpose:  To determine the awareness regarding contact lens related ocular complications among opticians of Lahore and to find out the relationship between the awareness and their educational qualification. Study Design:  Cross sectional survey. Place and duration of Study: University of Lahore from April 2020- December 2020. Methods:  This survey included opticians of different areas of Lahore, Pakistan. Two hundred and eleven opticians responded to a pre-tested, structured close ended questionnaire. Data comprised of demographics and contact lens selling experience. Information regarding awareness about contact lens related complications was also sought. The data were analyzed using SPSS 25.0. Results:  A total of 211 opticians participated in the survey. All subjects were males. Fifty four (25.6%) subjects were from the age group of 15 to 30 years, 122 (57.8%) were from the age group of 31 to 45 and 35 (16.6%) were from the age group of 46 to 60 years. Only 4.7% had contact lens dispensing diploma. Thirty seven percent patients had contact lens selling experience of 2 to 10 years. Similar percentage had more than 10 years of experience. Ten percent had less than one year of experience. Only 54 participants out of 211 had knowledge about dry eye, 9 knew about Acanthamoeba keratitis and Bacterial keratitis and only 2 were aware of corneal opacity. Conclusion:  Majority of the opticians of Lahore are unaware of the contact lenses related complications. Sale of cosmetic lenses for fashion purpose should be discouraged and banned if not prescribed by optometrist/ ophthalmologist. Key Words:  Opticians, Contact lenses, Bacterial Keratitis.

scholarly journals Acanthamoeba Keratitis among Rigid Gas Permeable Contact Lens Wearers in the United States, 2005 through 2011

Ophthalmology ◽  
2016 ◽  
Vol 123 (7) ◽  
pp. 1435-1441 ◽  
Author(s):  
Jennifer R. Cope ◽  
Sarah A. Collier ◽  
Oliver D. Schein ◽  
Allison C. Brown ◽  
Jennifer R. Verani ◽  
...  
Keyword(s):  
United States ◽  
Contact Lens ◽  
Acanthamoeba Keratitis ◽  
The United States

Acanthamoeba keratitis due to genotype T11 in a rigid gas permeable contact lens wearer in Spain

2011 ◽  
Vol 34 (2) ◽  
pp. 83-86 ◽  
Author(s):  
Jacob Lorenzo-Morales ◽  
Rafael Morcillo-Laiz ◽  
Carmen Mª Martín-Navarro ◽  
Rogelio López-Vélez ◽  
Atteneri López-Arencibia ◽  
...  
Keyword(s):  
Contact Lens ◽  
Acanthamoeba Keratitis ◽  
Contact Lens Wearer

The role of domestic tap water on Acanthamoeba keratitis in non-contact lens wearers and validation of laboratory methods

2015 ◽  
Vol 114 (9) ◽  
pp. 3283-3289 ◽  
Author(s):  
Ismail Soner Koltas ◽  
Fadime Eroglu ◽  
Elif Erdem ◽  
Meltem Yagmur ◽  
Ferdi Tanır
Keyword(s):  
Contact Lens ◽  
Tap Water ◽  
Acanthamoeba Keratitis ◽  
Laboratory Methods

scholarly journals Enhanced Killing of Acanthamoeba Cysts with a Plant Peroxidase-Hydrogen Peroxide-Halide Antimicrobial System

2003 ◽  
Vol 69 (5) ◽  
pp. 2563-2567 ◽  
Author(s):  
Reanne Hughes ◽  
Peter W. Andrew ◽  
Simon Kilvington
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Contact Lens ◽  
Acanthamoeba Keratitis ◽  
Free Living ◽  
Soybean Peroxidase ◽  
Plant Peroxidase ◽  
Time Points ◽  
Free Living Amoeba ◽  
Care Systems

ABSTRACT The activity of H2O2 against the resistant cyst stage of the pathogenic free-living amoeba Acanthamoeba was enhanced by the addition of KI and either horseradish peroxidase or soybean peroxidase or, to a lesser degree, lactoperoxidase. This resulted in an increase in the cysticidal activity of 3% (wt/vol) H2O2, and there was >3-log killing in 2 h, compared with the 6 h required for comparable results with the peroxide solution alone (P < 0.05). With 2% H2O2, enhancement was observed at all time points (P < 0.05), and total killing of the cyst inoculum occurred at 4 h, compared with 6 h for the peroxide alone. The activity of sublethal 1% H2O2 was enhanced to give 3-log killing after 8 h of exposure (P < 0.05). No enhancement was obtained when KCl or catalase was used as a substitute in the reaction mixtures. The H2O2 was not neutralized in the enhanced system during the experiments. However, in the presence of a platinum disk used to neutralize H2O2 in contact lens care systems, the enhanced 2% H2O2 system gave 2.8-log killing after 6 h or total cyst killing by 8 h, and total neutralization of the H2O2 occurred by 4 h. In contrast, 2% H2O2 alone resulted in <0.8-log killing of cysts in the presence of the platinum disk due to rapid (<1 h) neutralization of the peroxide. Our observations could result in significant improvement in the efficacy of H2O2 contact lens disinfection systems against Acanthamoeba cysts and prevention of acanthamoeba keratitis.

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