The Separation of the Long Chain Fatty Acids by Gas-Liquid Chromatography

1958 ◽  
Vol 6 (6) ◽  
pp. 595-600 ◽  
Author(s):  
A. T. JAMES
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Gas Liquid Chromatography ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

Analysis of Long-Chain Fatty Acids by Gas-Liquid Chromatography

1959 ◽  
Vol 31 (2) ◽  
pp. 307-308 ◽  
Author(s):  
Wilhelm. Stoffel ◽  
Florence. Chu ◽  
E. H. Ahrens
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Gas Liquid Chromatography ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

Long-chain fatty acids of peptococci and peptostreptococci

1976 ◽  
Vol 4 (6) ◽  
pp. 515-521
Author(s):  
C L Wells ◽  
C R Field
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Single Species ◽  
Gas Liquid Chromatography ◽  
Long Chain Fatty Acids ◽  
Whole Cells ◽  
Group I ◽  
Group Ii ◽  
Long Chain ◽  
Chain Fatty Acids

The long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. The resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into four groups. Fifteen strains of Peptostreptococcus anaerobius were placed in group I because they had a unique, prominent compound that occurred in the area where a C8 to C10 fatty acid would be expected. Group II, consisting of Peptostreptococcus intermedius, Peptostreptococcus micros, Peptostreptococcus parvulus, Peptococcus morbillorum, and Peptococcus constellatus, produced C14, C16:1, C18:1, and C18 fatty acids. Peptococcus prevotii, Peptococcus variabilus, Peptococcus magnus, Peptococcus asaccharolyticus, and Peptostreptococcus productus were placed in group III because they contained three to six additional, unidentified compounds that strikingly differentiated them from group II. Peptococcus saccharolyticus was the single species assigned to group IV because it yielded C14, C16, C18:1, C18, and C20 fatty acids and a prominent unidentified peak that occurred between C14 and C16 fatty acids. This study indicated that cellular long-chain fatty acids may be an important tool in clarifying the taxonomy of the peptococci and peptostreptococci.

Release of essential fatty acids from the rat mesenteric vascular bed perfused in vitro: modulation by zinc

1990 ◽  
Vol 68 (7) ◽  
pp. 903-907 ◽  
Author(s):  
Stephen C. Cunnane ◽  
Bassam A. Nassar
Keyword(s):  
Fatty Acids ◽  
Essential Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Long Chain Fatty Acids ◽  
Perfusion Medium ◽  
Vascular Bed ◽  
Mesenteric Vascular Bed ◽  
Long Chain ◽  
Chain Fatty Acids

The rat mesenteric vascular bed releases prostaglandins when perfused in vitro. The present study evaluated the effect of perfusion of the rat mesenteric vascular bed in vitro with a buffer containing 0, 3, 6, or 9 nM of added zinc on the release of essential fatty acids over a 150-min period. Long chain fatty acids in the mesenteric lipids and in total lipid of the perfusion effluent were assayed by gas liquid chromatography. The presence of 6 nM zinc in the perfusing buffer almost completely prevented the change in 16–22 carbon long chain fatty acids in the mesenteric phospholipids and decreased the release of free fatty acids in comparison to that occurring in the absence of additional zinc. The results sugest that physiological amounts of zinc in the perfusion medium reduce the release of essential fatty acids from rat mesenteric lipids.Key words: zinc, phospholipid, linoleic acid, arachidonic acid, prostaglandin.

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