A Simple Screening Method for Detecting Isovalerylglycine in Urine of Patients with Isovaleric Acidemia

1970 ◽  
Vol 16 (5) ◽  
pp. 420-422 ◽  
Author(s):  
Toshiyuki Ando ◽  
William L Nyhan
Keyword(s):  
Thin Layer ◽  
Metabolic Diseases ◽  
Neonatal Period ◽  
Screening Method ◽  
Isovaleric Acid ◽  
Isovaleric Acidemia ◽  
Simple Detection ◽  
Normal Urine ◽  
Layer Chromatography ◽  
Simple Screening

Abstract Isovaleric acidemia is one of an enlarging group of inherited metabolic diseases now recognized to cause death in the neonatal period. Rapid, simple detection is essential for diagnosis of these conditions. We describe a method that fulfills these criteria for isovaleric acidemia. Excretion of isovalerylglycine in the urine more reliably indicates the condition than the presence of volatile isovaleric acid in the blood. Isovalerylglycine is extracted from urine and separated by thin-layer chromatography from other constituents of urine. It is detected by spraying the chromatogram with bromcresol purple. Isovalerylglycine does not appear in normal urine.

Screening Method for the Detection of Aflatoxins, Ochratoxin, Patulin, Sterigmatocystin, and Zearalenone in Cereals

1977 ◽  
Vol 60 (6) ◽  
pp. 1369-1371 ◽  
Author(s):  
B G Egon Josefsson ◽  
Tord E Möller
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ochratoxin A ◽  
Screening Method ◽  
Gel Filtration ◽  
Limits Of Detection ◽  
Layer Chromatography

Abstract A screening method has been developed for the detection of aflatoxins, ochratoxin A, patulin, sterigmatocystin, and zearalenone in cereals. After extraction, the sample is cleaned up by gel filtration. The mycotoxins are separated by thin layer chromatography. The limits of detection are about 5 μg aflatoxins, 10 ochratoxin A, 50 μg patulin, 10 μg sterigmatocystin, and 35 μg zearalenone/kg.

Thin Layer Chromatographic Screening Method for Sulfadiazine Residues in Calf Tissues, Plasma, and Urine

1978 ◽  
Vol 61 (3) ◽  
pp. 545-549
Author(s):  
Joseph L Woolley ◽  
Oliver Murch ◽  
Carl W Sigel
Keyword(s):  
Thin Layer ◽  
Screening Method ◽  
Target Tissue ◽  
Highly Sensitive ◽  
Tissue Homogenates ◽  
Specific Tissue ◽  
Sulfonamide Residues ◽  
Kidney Liver ◽  
Layer Chromatography ◽  
Endogenous Substances

Abstract Because of the lack of specificity of the Bratton-Marshall procedure for assaying sulfonamides, a sensitive, specific tissue residue assay for sulfadiazine (SDZ) was developed. The methodology has been extended to provide a highly sensitive screen for sulfonamide residues, which employs 2-dimensional thin layer chromatography in conjunction with fluorescamine derivatization. The procedure described, which has been developed for SDZ in calf tissues, involves direct ethyl acetate extraction of tissue homogenates. Following evaporation of the organic phase, a portion of the residue is spotted on a 20x20 cm silica gel 60 plate, which is then developed in 2 dimensions with solvent systems devised to separate SDZ from endogenous substances as well as from 12 other sulfonamides that might be present in calf tissues. The presence of SDZ at a concentration of 0.1 ppm or its absence is easily demonstrated in calf kidney, liver, muscle, plasma, and urine. The basic method can be modified for a particular sulfonamide in a target tissue and can be used as a quantitative assay for sulfonamide residues.

scholarly journals Thin-Layer Chromatographic Determination of Monensin in Feeds: Screening Method

1998 ◽  
Vol 81 (4) ◽  
pp. 844-847 ◽  
Author(s):  
Wynne W Landgraf ◽  
P Frank Ross
Keyword(s):  
Solid Phase Extraction ◽  
Thin Layer ◽  
Solid Phase ◽  
Screening Method ◽  
Chromatographic Determination ◽  
Phase Extraction ◽  
Color Development ◽  
Layer Chromatography ◽  
Positive Results

Abstract Monensin is extracted from feed with methanol and purified by solvent-partitioning solid-phase extraction. After solvent reduction, monensin is separated by thin-layer chromatography on silica gel and visualized by color development with vanillin. No false-positive results were obtained in validation studies by submitting or peer laboratories when blank samples were analyzed. Three of 20 samples spiked with 5 ppm monensin were reported as containing no monensin. All samples spiked with 10 ppm monensin were reported positive for monensin.

High efficiency screening of nine lipid-lowering adulterants in herbal dietary supplements using thin layer chromatography coupled with surface enhanced Raman spectroscopy

2017 ◽  
Vol 9 (10) ◽  
pp. 1595-1602 ◽  
Author(s):  
Qingxia Zhu ◽  
Mengyun Chen ◽  
Lu Han ◽  
Yongfang Yuan ◽  
Feng Lu
Keyword(s):  
Raman Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Efficiency ◽  
Screening Method ◽  
Surface Enhanced Raman Spectroscopy ◽  
Lipid Lowering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Layer Chromatography

A high efficiency screening method was developed to analyze lipid-lowering adulterants in complicated HDS systems.

Rapid Screening Method for Deoxynivalenol in Agricultural Commodities by Fluorescent Minicolumn

1990 ◽  
Vol 73 (2) ◽  
pp. 266-270
Author(s):  
William C Gordon ◽  
Linda J Gordon
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Selective Adsorption ◽  
Screening Method ◽  
Rapid Screening ◽  
Blue Fluorescence ◽  
False Negatives ◽  
Rapid Screening Method ◽  
Gas Chromatography Mass Spectroscopy ◽  
Layer Chromatography

Abstract A rapid screening procedure based on the selective adsorption of deoxynivalenol (DON) from extracts of wheat and corn has been developed. DON is extracted from the sample with acetonitrile-water (85 + 15) and partially purified on a preparative minicolumn. Solvent is evaporated and the residue is dissolved in toluene-acetone (95 + 5) and chromatographed on a novel detector minicolumn which selectively adsorbs DON. A blue fluorescence is produced when the column is heated 5 min at 100°C. The procedure is capable of detecting DON at ≥ 500 ng/g. Forty-three wheat samples, contaminated with DON at 60-6300 ng/g, were assayed by gas chromatography-mass spectroscopy (GC-MS) of the heptafluorobutyryl derivative of DON and by the selective adsorption procedure. Comparison of results showed 9 1% agreement between data from the 2 methods. Selective adsorption assays were positive for all samples that were ≥ 500 ng/g by GC-MS (no false negatives) and were negative for 85 % of samples < 500 ng/g (4/27 false positives). These four samples contained > 200 ng/g by GC-MS. Samples of wheat (64), corn (23), soybeans (8), and sorghum (6) were extracted and extracts were assayed by thin-layer chromatography and the selective adsorption procedure. Selective adsorption assays agreed with TLC results.

A Screening Method for Sulfonamides Extracted from Animal Tissues

1975 ◽  
Vol 58 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Wendell F Phillips ◽  
John E Trafton
Keyword(s):  
Quantitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Screening Method ◽  
Qualitative And Quantitative Analysis ◽  
Animal Tissues ◽  
Qualitative And Quantitative ◽  
Poultry Tissues ◽  
Layer Chromatography

Abstract A screening method for the estimation of possible residues of sulfonamides in poultry tissues is described. The method utilizes thin layer chromatography (TLC) to separate Bratton-Marshall positive reactants. In the absence of interference and the identification of 1 sulfonamide by TLC, the colorimctric method is recommended for quantitation. When interferences are present, TLC should be used for both qualitative and quantitative analysis. The screening method has a sensitivity of less than 0.05 ppm and a recovery of greater than 80%.

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