Serum alkaline phosphatase measured by using thymol blue monophosphate and a bichromatic analyzer.

1976 ◽  
Vol 22 (10) ◽  
pp. 1737-1740 ◽  
Author(s):  
R C Elser
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Blood Donors ◽  
Serum Alkaline Phosphatase ◽  
Thymol Blue ◽  
Serum Alkaline Phosphatase Activity ◽  
Nitrophenyl Phosphate ◽  
Stable Substrate ◽  
High Concentrations

Abstract Thymol blue monophosphate is evaluated as a substrate for use in kinetic bichromatic analysis of serum alkaline phosphatase activity. The effect of abnormally high concentrations of hemoglobin, bilirubin, or chylomicrons is negligible. The expected range for ambulatory adults established by assaying sera from 206 blood donors, was 12-44 U/lliter at 37 degrees C. Comparison of the method with a commonly used p-nitrophenyl phosphate method yielded a correlation coefficient, r, of 0.9949. Advantages of the method are the reduced cost per test and the exceptionally stable substrate.

Use of N-methyl-D-glucamine as buffer in the determination of serum alkaline phosphatase activity.

1981 ◽  
Vol 27 (10) ◽  
pp. 1729-1732 ◽  
Author(s):  
V Chromý ◽  
L Zahradnícek ◽  
J Voznícek
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Reaction Conditions ◽  
Serum Alkaline Phosphatase Activity ◽  
Nitrophenyl Phosphate ◽  
Optimum Reaction ◽  
Assay Conditions

Abstract We describe a method for determining serum alkaline phosphatase activity with use of N-methyl-D-glucamine buffer, Na+ is a definite activator, whereas NH4+ and Li+ inhibit enzyme activity. Optimum reaction conditions are: methylglucamine buffer, 0.35 mol/L, pH 10.2 +/- 0.1 (30 degrees C); NaCl, 70 mmol/L; MgCl2, 0.5 mmol/L; disodium 4-nitrophenyl phosphate, 15 mmol/L; reaction temperature, 30 degrees C; reaction time, 2 min. The assay conditions are optimum for all human serum isoenzymes.

Alkaline phosphatase activity in the plasma of children and adolescents.

1977 ◽  
Vol 23 (3) ◽  
pp. 469-472 ◽  
Author(s):  
G A Fleisher ◽  
E S Eickelberg ◽  
L R Elveback
Keyword(s):  
Alkaline Phosphatase ◽  
Public Schools ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Intraclass Correlation ◽  
Serum Alkaline Phosphatase Activity ◽  
Upper Limits ◽  
Prepubertal Girls

Abstract We determined plasma (serum alkaline phosphatase activity in 854 healthy students of the Rochester, Minnesota, public schools. Prepubertal girls had somewhat greater upper limits than did boys, and there was a low trend of increasing activity in both sexes. At the beginning of adolescence increasing activities were observed, which peaked at ages 11 to 12 years in girls and at ages 13 to 14 in boys. Adult values were not reached until six to eight years later. In 180 pairs of siblings, a significant intraclass correlation was noted. A possible role of alkaline phosphatase in the regulation of protein synthesis is suggested.

Effects of various fibrates on serum alkaline phosphatase activity

2002 ◽  
Vol 165 (1) ◽  
pp. 187-188 ◽  
Author(s):  
Emanuel Ganotakis ◽  
Vasilios Tsimihodimos ◽  
Eleni Bairaktari ◽  
Evagelos Rizos ◽  
Vasilios Athyros ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase Activity
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