Rapid, fully automated radioimmunoassay of prostatic acid phosphatase in serum.

1980 ◽  
Vol 26 (11) ◽  
pp. 1583-1587 ◽  
Author(s):  
S Dass ◽  
N L Bowen ◽  
K D Bagshawe
Keyword(s):  
Acid Phosphatase ◽  
Prostatic Acid Phosphatase ◽  
Metastatic Carcinoma ◽  
Control Group ◽  
Control Groups ◽  
Carcinoma Of The Prostate ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Specific Antisera ◽  
Chloramine T

Abstract Prostatic acid phosphatase was purified from human semen and its purity established by biochemical and immunological criteria. Rabbits were injected with the purified isoenzyme to raise specific antisera. The prostatic acid phosphatase was radiolabeled with 125I by the Chloramine T method. We developed a fully automated double-antibody radiommunosassay for measuring prostatic acid phosphatase in serum from patients with carcinoma of the prostate and from several control groups. The lower detection limit of the radioimmunoassay was 2.0 microgram of prostatic acid phosphatase per litre of serum. Values for most members of the control group was <2.0 microgram/L; patients with metastatic carcinoma of the prostate had values ranging from <2.0 to 300 microgram/L of serum.

Comparison of Bolton-Hunter and Chloramine-T Techniques for the Radioiodination of Prostatic Acid Phosphatase

1983 ◽  
Vol 20 (2) ◽  
pp. 112-115 ◽  
Author(s):  
C M Sturgeon ◽  
L Beynon ◽  
J Seth ◽  
G D Chisholm
Keyword(s):  
Acid Phosphatase ◽  
Detection Limit ◽  
Prostatic Acid Phosphatase ◽  
Chromatographic Purification ◽  
Double Antibody ◽  
Chloramine T

A double antibody, semi-automated radioimmunoassay for serum prostatic acid phosphatase (PAP) is described, which uses the 125I-labelled N-succinimidyl-3-(4-hydroxyphenyl) propionate ester of PAP. This type of label has substantially higher immunoreactivity than that prepared using the chloramine-T method of radioiodination. Chromatographic purification of either label on Ultrogel ACA44 further improved immunoreactivity. The lowest detection limit (0·35 μg/l) was achieved with the chromatographically purified ester label.

Measurements of serum gamma-seminoprotein and prostate specific antigen evaluated for monitoring carcinoma of the prostate.

1986 ◽  
Vol 32 (11) ◽  
pp. 2040-2043 ◽  
Author(s):  
J K Siddall ◽  
S D Shetty ◽  
E H Cooper
Keyword(s):  
Acid Phosphatase ◽  
Disease Progression ◽  
Prostate Specific Antigen ◽  
Prostatic Cancer ◽  
Specific Antigen ◽  
Prostatic Acid Phosphatase ◽  
Carcinoma Of The Prostate ◽  
Normal Limits ◽  
Bony Metastases

Abstract We have compared the concentrations in serum of gamma-seminoprotein (gamma-SM) and prostate specific antigen (PSA), two antigens of prostatic origin that are synthesized independently of prostatic acid phosphatase (PAP, EC 3.1.3.2), to assess their potential in monitoring prostatic cancer. At presentation, 27/30 (90%) patients with metastases had a PSA concentration greater than 10 ng/mL, and 29/30 (97%) a gamma-SM concentration greater than 10 ng/mL; 21/61 (34%) with disease but without metastases had an abnormal content of PSA, and 23/61 (38%) an abnormal gamma-SM. Concentrations of PSA and gamma-SM were significantly correlated (r = 0.68, p less than 0.001). In 20 patients without metastases followed longitudinally, the median concentrations of gamma-SM, PSA, and PAP in the 13 patients who developed bony metastases or showed signs of local spreading of the tumor were 58 ng/mL, 34 ng/mL, and 2.1 U/L, respectively. The corresponding median values in the seven patients who remained clinically stable were 2.5 and 3.9 ng/mL, and 2.3 U/L. We conclude that either PSA or gamma-SM can warn of disease progression when PAP activities are still within normal limits.

Prostate Specific Antigen and Prostatic Acid Phosphatase for Monitoring Therapy of Carcinoma of the Prostate

1991 ◽  
Vol 146 (4) ◽  
pp. 1064-1067 ◽  
Author(s):  
Andre Dupont ◽  
Leonello Cusan ◽  
Jose-Luis Gomez ◽  
Marie-Marthe Thibeault ◽  
Marjolaine Tremblay ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Prostatic Acid Phosphatase ◽  
Carcinoma Of The Prostate ◽  
Monitoring Therapy
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