An interlaboratory study of the determination of digoxin by immunoassay.

1980 ◽  
Vol 26 (3) ◽  
pp. 480-486 ◽  
Author(s):  
D M Fast ◽  
W H Hannon ◽  
C A Burtis ◽  
D D Bayse
Keyword(s):  
Frequency Distribution ◽  
Statistical Difference ◽  
Analytical Data ◽  
Statistical Information ◽  
Mean Values ◽  
Clinical Laboratories ◽  
The Mean ◽  
Computational Procedures

Abstract We conducted a voluntary survey of laboratories and manufacturers to assess the current quality of analytical assays for serum digoxin. More than 300 clinical laboratories and 18 manufacturers responded, giving data on methods, instruments, computational procedures, and results for five survey samples. We sorted the analytical data to provide statistical information on the grand mean values separately for manufacturers and clinical laboratories, the frequency distribution of all reported values, and the mean values by method of interpolation and algorithms used for linear transformation. There was no statistical difference (alpha = 0.05) between the means for each specimen as determined by the kit manufacturers as a group and the clinical laboratories as a group.

An interlaboratory study of the determination of digoxin by immunoassay.

1980 ◽  
Vol 26 (3) ◽  
pp. 480-486
Author(s):  
D M Fast ◽  
W H Hannon ◽  
C A Burtis ◽  
D D Bayse
Keyword(s):  
Frequency Distribution ◽  
Statistical Difference ◽  
Analytical Data ◽  
Statistical Information ◽  
Mean Values ◽  
Clinical Laboratories ◽  
The Mean ◽  
Computational Procedures

Abstract We conducted a voluntary survey of laboratories and manufacturers to assess the current quality of analytical assays for serum digoxin. More than 300 clinical laboratories and 18 manufacturers responded, giving data on methods, instruments, computational procedures, and results for five survey samples. We sorted the analytical data to provide statistical information on the grand mean values separately for manufacturers and clinical laboratories, the frequency distribution of all reported values, and the mean values by method of interpolation and algorithms used for linear transformation. There was no statistical difference (alpha = 0.05) between the means for each specimen as determined by the kit manufacturers as a group and the clinical laboratories as a group.

scholarly journals pH of exudate test in the physiological quality of crambe seeds

2016 ◽  
Vol 46 (6) ◽  
pp. 1014-1018 ◽  
Author(s):  
Charline Zaratin Alves ◽  
Lennis Afraire Rodrigues ◽  
Carlos Henrique Queiroz Rego ◽  
Josué Bispo da Silva
Keyword(s):  
Pearson Correlation ◽  
Raw Material ◽  
Mean Values ◽  
Tukey Test ◽  
Physiological Quality ◽  
The Subject ◽  
The Mean ◽  
C 30

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.

scholarly journals The Quality of Statistical Reporting and Data Presentation in Predatory Dental Journals Was Lower Than in Non-Predatory Journals

Entropy ◽  
2021 ◽  
Vol 23 (4) ◽  
pp. 468
Author(s):  
Pentti Nieminen ◽  
Sergio E. Uribe
Keyword(s):  
Open Access ◽  
Peer Review ◽  
Scientific Journals ◽  
Mean Values ◽  
Data Presentation ◽  
Predatory Journals ◽  
Study Results ◽  
The Mean ◽  
Highly Cited

Proper peer review and quality of published articles are often regarded as signs of reliable scientific journals. The aim of this study was to compare whether the quality of statistical reporting and data presentation differs among articles published in ‘predatory dental journals’ and in other dental journals. We evaluated 50 articles published in ‘predatory open access (OA) journals’ and 100 clinical trials published in legitimate dental journals between 2019 and 2020. The quality of statistical reporting and data presentation of each paper was assessed on a scale from 0 (poor) to 10 (high). The mean (SD) quality score of the statistical reporting and data presentation was 2.5 (1.4) for the predatory OA journals, 4.8 (1.8) for the legitimate OA journals, and 5.6 (1.8) for the more visible dental journals. The mean values differed significantly (p < 0.001). The quality of statistical reporting of clinical studies published in predatory journals was found to be lower than in open access and highly cited journals. This difference in quality is a wake-up call to consume study results critically. Poor statistical reporting indicates wider general lower quality in publications where the authors and journals are less likely to be critiqued by peer review.

SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

1987 ◽  
Author(s):  
J Grøndahl-HANSEN ◽  
N Agerlin ◽  
L S Nielsen ◽  
K Danø
Keyword(s):  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mean Values ◽  
Amino Terminal ◽  
Type Plasminogen Activator ◽  
Healthy Donors ◽  
Urokinase Type Plasminogen Activator ◽  
The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

scholarly journals AB0101 THE IMPACT OF TIME SPAN TO ACHIEVE BOOLEAN REMISSION FOR MAINTAINING DISEASE ACTIVITY AFTER ACQUISITION IN RHEUMATOID ARTHRITIS PATIENT

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1079.1-1079
Author(s):  
I. Yoshii
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Disease Duration ◽  
Remission Rate ◽  
Mean Value ◽  
Time Span ◽  
Mean Values ◽  
The Mean ◽  
The Relationship

Background:Boolean remission criteria is one most popular and stringent criteria in treating patient with rheumatoid arthritis (RA), because it may guarantees a stable clinical course after attaining remission.Objectives:Impact of time span from initiation to achieving Boolean remission on maintaining disease activity, daily activities, and quality of life after attaining Boolean remission was investigated from daily clinical practice data.Methods:685 patients with RA since August 2010 under the T2T strategy were treated. They were monitored for their TJC, SJC, PGA, EGA, CRP, and disease activity indices such as CDAI, SDAI, DAS28, and Boolean criteria at every visit. HAQ-DI score, pain score using visual analog scale (PS-VAS), and EQ-5D were also monitored, and the quality of life score (QOLS) calculated from EQ-5D was determined at every visit from the time of diagnosis (baseline).Of 685 patients, 465 patients had achieved Boolean remission >1 times, and were consecutively followed up for >3 years. These patients were enrolled in the study. Time span from the first visit to first Boolean remission was calculated. The relationship between the time span and each of background parameters, and the relationship between the time span and each of the mean values of the SDAI score, HAQ score, PS-VAS, SHS, and QOLS at the first Boolean remission and thereafter was evaluated statistically.Patients were subsequently divided into the G ≤ 6 and G > 6 groups based on the achievement of first Boolean remission within two groups: time span G ≤ 6 months and G > 6 months. The two groups were compared with regard to the SDAI score, HAQ score, PS-VAS, SHS, and QOLS at first visit and at the time of first Boolean remission, and the mean values of these parameters after remission were evaluated statistically. Moreover, changes of these parameters and the mean Boolean remission rate after the first remission, and SDAI remission rate at the first Boolean remission to thereafter were compared between the two groups statistically.Results:Out of 465 patients, females comprised 343 (73.7%), and the mean age was 67.8 years (range, from 21–95 years). The mean disease duration at first visit was 6.1 years (range, from 1 months–45 years). The mean follow up length was 88.1 months (range: 36–122 months; median: 85 months) and mean time span from the first visit to the first Boolean remission was 8.1 months. The mean SDAI score, HAQ score, PS-VAS, and the QOLS at first visit were 13.3, 0.467, 33.2, and 0.834, respectively. Among the study parameters, PS-VAS and QOLS were significantly correlated with the time span. For parameters at the first Boolean remission, HAQ-DI score, PS-VAS, and QOLS demonstrated significant correlation with the time span, whereas SDAI, HAQ-DI score, PS-VAS, SHS, and QOLS after the Boolean remission demonstrated significant correlation with the time span.The comparison between the G ≤ 6 and the G > 6 groups revealed that the disease duration, HAQ score, and PS-VAS at baseline in the G > 6 were significantly higher than that in the G ≤ 6 group, and QOLS in the G ≤ 6 group was significantly higher than that in the G > 6 group at baseline. Similarly, the HAQ score and PS-VAS at the first Boolean remission in the G > 6 group were significantly higher than that in the G ≤ 6 group, whereas QOLS in the G ≤ 6 group demonstrated no significant difference compared with that in the G > 6 group.The mean value of the SDAI score after the first Boolean remission in the G > 6 group was significantly higher than that in the G ≤ 6 group. Similarly, the SDAI score, HAQ score, and PS-VAS after the first Boolean remission in the G > 6 group were also significantly higher than those in the G ≤ 6 group, and the mean value of the QOLS in the G ≤ 6 group were significantly higher than that in the G > 6 group. The Boolean remission rate and SDAI remission rate after the first Boolean remission were significantly higher in the G ≤ 6 group than those in the G > 6 group.Conclusion:Attaining Boolean remission ≤ 6 months for RA has significant benefit for more stable disease control, that leads good maintenance of ADL.Disclosure of Interests:None declared

The Determination of the Probabilistic Properties of Velocities and Accelerations in Kinematic Chains With Uncertainty

1989 ◽  
Author(s):  
S. J. Lee ◽  
B. J. Gilmore
Keyword(s):  
Effective Length ◽  
Radial Clearance ◽  
Probabilistic Design ◽  
Link Length ◽  
Mean Values ◽  
Kinematic Chains ◽  
Analysis Package ◽  
The Mean ◽  
Linkage Model

Abstract A probabilistic model and methods to determine the means and variances of the velocity and acceleration of stochastically-defined planar pin jointed kinematic chains are presented. The presented model considers the effect of tolerances on link length and radial clearance and uncertainty of pin location as a net effect on the link’s effective length. The determination of the mean values and variances of the output variables requires the calculation of sensitivities of secondary variables with respect to the random variables. It is shown that this computation is straightforward and can be accomplished by a conventional kinematic analysis package. Thus, the concepts of tolerance and clearance have been captured by the model and analysis. The only input data is the nominal linkage model and statistical information. The “effective link length” model is shown to be applicable to both analytical solution and Monte Carlo simulation. The results from both methods are compared. This paper solves the higher-order kinematics problem for the probabilistic design analysis of stochastically defined mechanisms.

scholarly journals Interlaboratory Comparison for the Determination of Five Residual Organochlorine Pesticides in Ginseng Root Samples by Gas Chromatography

2007 ◽  
Vol 90 (4) ◽  
pp. 1133-1141 ◽  
Author(s):  
Mei-Fung Kong ◽  
Serena Chan ◽  
Yiu-Chung Wong ◽  
Siu-Kay Wong ◽  
Della Wai-Mei Sin
Keyword(s):  
Measurement Uncertainty ◽  
Organochlorine Pesticides ◽  
Interlaboratory Comparison ◽  
Analytical Data ◽  
Quality Criterion ◽  
Asia Pacific ◽  
Ginseng Root ◽  
Mean Values ◽  
Z Scores

Abstract An interlaboratory comparison study for the determination of 5 residual organochlorine pesticides (hexachlorobenzene and 4 hexachlorocyclohexane isomers) in ginseng root was performed. This program [Asia Pacific Laboratory Accreditation Cooperation (APLAC) T049] was the first of its kind for an herbal matrix and involved the participation of 70 laboratories from 26 countries worldwide. Consensus mean values were computed statistically from the reported results, which were eventually used to assess the performance of individual laboratories in terms of the z-scores. The distribution of analytical data was found to be widespread, with standard deviation ranging from 43.9 to 55.9%, and the result patterns obtained were similar to those residue pesticide programs using other matrixes. Although the estimation of measurement uncertainty is a crucial requirement for all quantitative tests for laboratories that meet the requirements of International Organization for Standardization/International Electrotechnical Commisssion (ISO/IEC) 17025, some laboratories in this program had difficulties and showed unfamiliarity with respect to that quality criterion. It was recommended that laboratories review and rectify the situation promptly so that they would have a better understanding of measurement uncertainty or the test service provided.

P6489Preoperative determination of artificial chordae length by transesophageal echocardiography

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
A Pitsis ◽  
N Tsotsolis ◽  
E Theofilogiannakos ◽  
H Boudoulas ◽  
K Boudoulas
Keyword(s):  
Mitral Valve ◽  
Mitral Valve Repair ◽  
Pearson Correlation ◽  
Transoesophageal Echocardiography ◽  
Valve Repair ◽  
Bottom Part ◽  
Artificial Chordae ◽  
The Mean

Abstract The use of artificial chordae is one of the main techniques used in mitral valve repair to treat prolapsing leaflets, especially in anterior and bileaflet prolapse. With the use of PTFE sutures to replace elongated or ruptured chords mitral valve repair rates have significantly improved. The main difficulty with this technique is to determine the optimal length of the artificial chordae. Intraoperative transoesophageal echocardiography (TOE) is mandatory in mitral valve repair in order to determine the type of lesion of the mitral valve but also to evaluate the quality of the repair. We examined the accuracy of preoperative prediction of artificial chordae length by the preoperativeTOE. Patients and methods Twenty-one consecutive patients (7 females) underwent mitral valve repair with artificial chordae for significant mitral valve prolapse in our department during the last year. The median age of the patients was 62 y. (range 25 - 87) and the mean EuroSCORE II 3,36% (SD 4,61%). During the prep TOE we determined the predicted length of the required replacement chordae for the repair using mainly the 4 chamber view to calculate the distance between the tip of the papillary muscle and the coaptation point of the two leaflets and we subtracted 5mm which is the minimum of the desired coaptation length (top right part of the Figure). All the patients underwent totally endoscopic mitral repair through a 3 cm right periareolar incision. 14,3% of the patients had anterior leaflet (AML) prolapse, 23,8% bileaflet and 61,9% posterior (PML). The appropriate length of the required chordae was measured intraoperatively. Results We used on average 3 loops of artificial chordae size 23,3 mm (SD 1,15mm) to treat the AML prolapse, 5 loops size 23,2 mm (SD 2,28mm) to treat the bileaflet prolapse and 2,23 loops size 18mm (SD 2mm) to treat the PML prolapse (bottom part of the figure). The predicted size of the artificial chordae had a positive correlation to the length used (Pearson correlation, p<0,001) as demonstrated in the top left part of the Figure. An annuloplasty band was implanted to all the patients. All the patients had no mitral regurgitation in the postoperative TOE. The mean valve area was 3,54cm2 (SD 0,57) and the mean peak gradient 5,6 mmHg (SD 1,82). There was no mortality. Chordae length predicted by echo Conclusion The length of artificial chordae can be predetermined with great accuracy with the use of TOE, making TOE an important tool not only for the determination of the mitral lesion and quality of the repair but also for the planning of the operation.

Colorimetric Method for Rapid Determination of Serum Arginase

1967 ◽  
Vol 13 (10) ◽  
pp. 900-908 ◽  
Author(s):  
Brigitta Mellerup
Keyword(s):  
Rapid Determination ◽  
Colorimetric Method ◽  
Normal Range ◽  
Clinical Routine ◽  
Mean Values ◽  
Enzymatic Formation ◽  
Significant Difference ◽  
The Mean ◽  
Normal Men

Abstract A method for the determination of serum arginase is given which combines the enzymatic formation of urea with the sensitive method of Coulombe (1) for measuring this substance. This procedure allows more accurate determinations in the normal range than do previous methods described and is convenient for clinical routine. Significant difference is found between the mean values of normal men and women, 3.9 units/L. for the former and 2.9 units/L. for the latter.

scholarly journals Determination of carcinoembryonic antigen and cancer antigen values with the radioimmunoassay method in healthy females dogs

2013 ◽  
Vol 58 (No. 5) ◽  
pp. 277-283 ◽  
Author(s):  
V. Ledecky ◽  
A. Valencakova-Agyagosova ◽  
J. Lepej ◽  
Z. Frischova ◽  
S. Hornak ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Statistical Significance ◽  
Average Weight ◽  
Cancer Antigen ◽  
Cancer Markers ◽  
Mean Values ◽  
The Mean ◽  
Radioimmunoassay Method ◽  
Healthy Females

The aim of this study was to determine reference values of carcinoembryonic antigen and cancer antigen in 32 clinically healthy bitches. The average age of the bitches in each group was as follows: small breeds 3.50 &plusmn; 2.30, medium breeds 3.83 &plusmn; 3.21, large breeds 6.00 &plusmn; 3.22 and giant breeds 2.40 &plusmn; 2.43. The average weight in each group was as follows: 1<sup>st</sup> group 7.94 kg &plusmn; 1.84, 2<sup>nd</sup> group 22.38 kg &plusmn; 2.77, 3<sup>rd</sup> group 35.94 kg &plusmn; 7.16, and 4<sup>th</sup>&nbsp;group 52.75 kg &plusmn; 5.04. The cancer markers were determined using human kits. The mean values of the carcinoembryotic antigen markers &plusmn; SD were as follows: 1<sup>st</sup> group 0.18 &plusmn; 0.03, 2<sup>nd</sup> group 0.20 &plusmn; 0.03, 3<sup>rd</sup> group 0.22 &plusmn; 0.01, 4<sup>th</sup> group 0.18 &plusmn; 0.04. The statistical significance for the carcinoembryonic antigen markers was P = 0.0042**. The values of cancer antigen markers &plusmn; SD were: 4.90 &plusmn; 1.04, 4.80 &plusmn; 1.13, 5.90 &plusmn; 1.22, and 4.72 &plusmn; 0.97, respectively. The cancer antigen values were statistically insignificant (P = 0.1762). Based on obtained values of the mean 95%, we expect a standard for carcinoembryonic antigen of 0.00&ndash;0.23 ng/ml and for cancer antigen 0.0&ndash;7.00 IU/ml. The results of the present study show that it is possible to use human kits for the determination of carcinoembryonic antigen and cancer antigen in clinically healthy bitches using the radioimmunoassay method. &nbsp;

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