Enzymic fluorometric assay of plasma ammonia with a centrifugal analyzer.

1984 ◽  
Vol 30 (10) ◽  
pp. 1670-1672 ◽  
Author(s):  
M R Rodger ◽  
P Jenkins

Abstract This method for rapid plasma ammonia determination, supplied in the form of a commercially available kit, is based on a combination of enzymic reaction and fluorometry. Concentrations up to 350 mumol/L can be measured. The detection limit is 15 mumol/L. Intra-assay coefficients of variation for 30 and 100 mumol/L concentrations were 5.0% and 3.0%, respectively. The corresponding interassay CVs were 8.0% and 3.8%. Assay of 16 samples takes about 15 min. Only 25 microL of sample is required, which makes this assay suitable for pediatric use and small-animal research.

2005 ◽  
Author(s):  
Eric Potter ◽  
Fabrice Ouandji ◽  
Yuhua Li ◽  
Pascal Laignier ◽  
Preeti Kshirsagar ◽  
...  

2010 ◽  
Vol 15 (1) ◽  
pp. 016023 ◽  
Author(s):  
Fartash Vasefi ◽  
Michelle Belton ◽  
Bozena Kaminska ◽  
Glenn H. Chapman ◽  
Jeffrey J. L. Carson

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