Luminometric assays of seven acute-phase proteins in minimal volumes of serum, plasma, sputum, and bronchioalveolar lavage.

Abstract We describe immunoluminometric assays for seven acute-phase proteins, which can be determined in minimal volumes of plasma, serum, sputum, and bronchioalveolar lavage. The theoretical volume of serum or plasma required to measure all seven analytes in duplicate is 130 nL, although in practice the smallest volume of sample was enough to fill a hematocrit tube (about 25 microL of blood), collected from neonates by the heel-prick method. The assays could be performed with 10 microL of sputum or with 100 microL of bronchioalveolar lavage. We measured alpha 1-antitrypsin, alpha 2-macroglobulin, alpha 1-acid glycoprotein, thyroxin-binding prealbumin, C-reactive protein, and total and secretory immunoglobulin A. The assays are rapid enough for all results to be returned to the ward on the same day and are suitable for monitoring neonatal sepsis. All coefficients of variation, derived from compound precision profiles, were less than 7% for clinically relevant analyte concentrations. Correlation with commercially available nephelometric assays was good.

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The Acute Phase Proteins Reaction in Children Suffering from Pseudocroup

Mediators of Inflammation ◽

10.1155/2019/6518308 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7

Author(s):

Beata Pucher ◽

Magdalena Sobieska ◽

Michal Grzegorowski ◽

Jaroslaw Szydlowski

Keyword(s):

Acute Phase ◽

Inflammatory Reaction ◽

Acute Phase Proteins ◽

Control Group ◽

C Reactive Protein ◽

Acid Glycoprotein ◽

Reactive Protein ◽

Persistent Inflammation ◽

Alpha 1 Antitrypsin

The aim of the study was to evaluate the inflammatory reaction in children with pseudocroup and compare it with other laryngological diseases according to the available literature data. The study group included 51 children hospitalized because of pseudocroup. The measurements of the acute phase proteins (APP), such as C-reactive protein (CRP), alpha-1-antitrypsin (AT), alpha-1-antichymotrypsin (ACT), alpha-1-acid glycoprotein (AGP), ceruloplasmin (Cp), transferrin (Tf), alpha-2-macroglobulin (A2M), and haptoglobin (Hp) were obtained at 3 time points. The glycosylation profiles of AGP, ACT, and Tf were completed. An increased AGP level was observed in girls. The AGP glycosylation revealed the advantage of the W0 variant over the W1 variant. W1 and W2 were decreased in boys. W3 emerged in boys. The Tf concentration and T4 variant were lower compared to the control group. The A2M level was lower after treatment. The Hp and AT levels were decreased a few weeks later. The ACT glycosylation revealed a decrease of the A4 variant in boys. In conclusion, the inflammatory reaction during pseudocroup was of low intensity. The APP glycosylation suggested a chronic process. In a follow-up investigation, no normalization of the parameters was noted, but signs of persistent inflammation were observed.

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THE RELATIONSHIP BETWEEN THE COMPLEX OF IMMUNOGLOBULIN A AND ALPHA-1-ANTITRYPSIN, ITS CONSTITUENT COMPONENTS AND THE ACUTE-PHASE RESPONSE AS MEASURED BY C-REACTIVE PROTEIN IN RHEUMATOID ARTHRITIS TREATED WITH GOLD OR D-PENICILLAMINE

Rheumatology ◽

10.1093/rheumatology/26.5.351 ◽

1987 ◽

Vol 26 (5) ◽

pp. 351-353 ◽

Cited By ~ 10

Author(s):

P. T. DAWES ◽

R. JACKSON ◽

M. F. SHADFORTH ◽

I. V. LEWIN ◽

D. R. STANWORTH

Keyword(s):

Rheumatoid Arthritis ◽

Acute Phase ◽

Acute Phase Response ◽

Immunoglobulin A ◽

Phase Response ◽

C Reactive Protein ◽

Reactive Protein ◽

Alpha 1 Antitrypsin ◽

The Relationship

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Properties of four acute phase proteins: C-reactive protein, serum amyloid a protein, α1-acid glycoprotein, and fibrinogen

Seminars in Arthritis and Rheumatism ◽

10.1016/0049-0172(90)90055-k ◽

1990 ◽

Vol 20 (3) ◽

pp. 129-147 ◽

Cited By ~ 171

Author(s):

Duane R. Schultz ◽

Patricia I. Arnold

Keyword(s):

Acute Phase ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Serum Amyloid ◽

C Reactive Protein ◽

Acid Glycoprotein ◽

Reactive Protein ◽

Amyloid A ◽

Serum Amyloid A Protein ◽

Protein Serum

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Impact of Roux-en-Y gastric bypass on lipid and inflammatory profiles

Revista do Colégio Brasileiro de Cirurgiões ◽

10.1590/0100-69912015005007 ◽

2015 ◽

Vol 42 (5) ◽

pp. 305-310 ◽

Cited By ~ 5

Author(s):

Cleiton da Silva Oliveira ◽

Bruna Teles Soares Beserra ◽

Raphael Salles Granato Cunha ◽

Ana Gabriela Estevam Brito ◽

Rafaella Cristina Dimbarre de Miranda ◽

...

Keyword(s):

Gastric Bypass ◽

Total Cholesterol ◽

Acute Phase ◽

Acute Phase Proteins ◽

Plasma Concentrations ◽

C Reactive Protein ◽

Acid Glycoprotein ◽

Reactive Protein ◽

Significant Difference ◽

A Prospective Study

Objective: To evaluate the behavior of acute phase proteins and lipid profile in patients undergoing Roux-en-Y gastric bypass. Methods : We conducted a prospective study, consisting of three moments: M1 - preoperative (24 hours before surgery); M2 - 30 days after surgery; and M3 - 180 days after surgery. We carried measured height and BMI, as well as determined the concentrations of acute phase proteins (C-reactive protein (CRP), albumin and Alpha-1-acid glycoprotein) and total cholesterol, LDL-c, HDL-c and triacylglycerol. Results : participants comprised 25 individuals, with a mean age of 39.28 ± 8.07, 72% female. At all times of the study there was statistically significant difference as for weight loss and BMI. We found a significant decrease in CRP concentrations between the moments M1 and M3 (p = 0.041) and between M2 and M3 (p = 0.018). There was decrease in Alpha-1-GA concentrations between M1 and M2 (p = 0.023) and between M1 and M3 (p = 0.028). The albumin values increased, but did not differ between times. Total cholesterol and triacylglycerol decreased significantly ay all times. LDL-c concentrations decreased and differed between M1 and M2 (p = 0.001) and between M1 and M3 (p = 0.001). HDL-c values increased, however only differing between M1 and M2 (p = 0.050). Conclusion : Roux-en-Y gastric bypass promoted a decrease in plasma concentrations of CRP and Alpha-1-acid glycoprotein, improving lipid and inflammatory profiles.

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Serum Total Sialic Acid and Lipid-Associated Sialic Acid in Normal Individuals and Patients with Myocardial Infarction, and Their Relationship to Acute Phase Proteins

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329303000406 ◽

1993 ◽

Vol 30 (4) ◽

pp. 383-386 ◽

Cited By ~ 40

Author(s):

M Haq ◽

S Haq ◽

P Tutt ◽

M Crook

Keyword(s):

Myocardial Infarction ◽

Sialic Acid ◽

Acute Phase ◽

Acute Phase Proteins ◽

C Reactive Protein ◽

Acid Glycoprotein ◽

Reactive Protein ◽

Total Sialic Acid ◽

Normal Individuals ◽

The Mean

Serum total sialic acid (TSA) has gained medical interest, particularly as a cardiovascular risk factor and it has been hypothesized that serum levels relate to serum acute phase proteins, some of which are sialylated. We assayed serum TSA and also lipid associated sialic acid (LASA) in SO normal individuals (24 male) and IS subjects (12 male) who had experienced a myocardial infarct. The mean serum TSA in the normal individuals was 2·05 SD 0·38 mmol/L (range 1·16–2·74) and the mean serum LASA was 0.70 SD 0·19 mmol/L (range 0·23–1·03). We also measured five serum acute phase proteins and found a good correlation between these and serum TSA: C-reactive protein, r = 0.52, P<0.001, α-1-antichymotrypsin, r=0·79, P<0·0001, α-2-macroglobulin, r=0·38, P<0·01 and α-1-acid glycoprotein, r=0·32, P<0·05. A significant correlation between plasma TSA and plasma C-reactive protein ( r = 0·47, P<0·04) and also Fibrinogen ( r=0·53, P<0·04) was noted on day one following the myocardial infarction, whereas a significant correlation between plasma TSA and plasma α-1-antichymotrypsin ( r=0·51, P<0·03) and also plasma α-1-acid glycoprotein ( r=0·64, P<0·05) was found on day two following the infarction. Thus it would seem that serum TSA is at least in part related to some of the acute phase proteins in both healthy individuals and those having had a myocardial infarction.

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The acute phase response and C-reactive protein

Oxford Textbook of Medicine ◽

10.1093/med/9780198746690.003.0239 ◽

2020 ◽

pp. 2199-2207

Author(s):

Mark B. Pepys

Keyword(s):

Acute Phase ◽

Acute Phase Response ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Phase Response ◽

Serum Amyloid ◽

C Reactive Protein ◽

Reactive Protein ◽

Amyloid A ◽

Serum Amyloid A Protein

The acute phase response—trauma, tissue necrosis, infection, inflammation, and malignant neoplasia induce a complex series of nonspecific systemic, physiological, and metabolic responses including fever, leucocytosis, catabolism of muscle proteins, greatly increased de novo synthesis and secretion of a number of ‘acute phase’ plasma proteins, and decreased synthesis of albumin, transthyretin, and high- and low-density lipoproteins. The altered plasma protein concentration profile is called the acute phase response. Acute phase proteins—these are mostly synthesized by hepatocytes, in which transcription is controlled by cytokines including interleukin 1, interleukin 6, and tumour necrosis factor. The circulating concentrations of complement proteins and clotting factors increase by up to 50 to 100%; some of the proteinase inhibitors and α‎1-acid glycoprotein can increase three- to fivefold; but C-reactive protein (CRP) and serum amyloid A protein (an apolipoprotein of high-density lipoprotein particles) are unique in that their concentrations can change by more than 1000-fold. C-reactive protein—this consists of five identical, nonglycosylated, noncovalently associated polypeptide subunits. It binds to autologous and extrinsic materials which contain phosphocholine, including bacteria and their products. Ligand-bound CRP activates the classical complement pathway and triggers the inflammatory and opsonizing activities of the complement system, thereby contributing to innate host resistance to pneumococci and probably to recognition and safe ‘scavenging’ of cellular debris. Clinical features—(1) determination of CRP in serum or plasma is the most useful marker of the acute phase response in most inflammatory and tissue damaging conditions. (2) Acute phase proteins may be harmful in some circumstances. Sustained increased production of serum amyloid A protein can lead to the deposition of AA-type, reactive systemic amyloid.

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Acute phase proteins in bitches subjected to conventional and minimally invasive ovariohysterectomy

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5132 ◽

2018 ◽

Vol 38 (11) ◽

pp. 2124-2128 ◽

Cited By ~ 1

Author(s):

Elizabeth M.S. Schmidt ◽

Camila P. Rubio ◽

Funmilola Thomas ◽

João C.P. Ferreira ◽

David P. Eckersall

Keyword(s):

Minimally Invasive ◽

Acute Phase ◽

Acute Phase Proteins ◽

C Reactive Protein ◽

Reactive Protein ◽

Serum Haptoglobin ◽

Hemoglobin Binding ◽

Mixed Breed ◽

Significant Difference ◽

Haptoglobin Concentration

ABSTRACT: The aim of this study was to evaluate and to compare the possible inflammatory changes by screening acute phase proteins concentrations in healthy bitches subjected to ovariohysterectomy. Minimally invasive and conventional (laparotomy) ovariohysterectomies were performed in 17 client-owned adult female mixed breed dogs. Nine animals were subjected to minimally invasive and eight animals to conventional ovariohysterectomy. Blood samples were taken before surgery, 24, 48 hours, and seven days postoperatively. Serum C-reactive concentration was determined by a commercial ELISA kit and serum haptoglobin concentration was measured via hemoglobin binding assay, both previously validated for use in dogs. As the data did not meet the normal distribution criteria, the nonparametric Kruskall-Wallis was performed to compare quantitative variables between groups. One-way ANOVA and the Friedman test were used for multiple comparisons between time points, with a P<0.05 considered significant. C-reactive protein concentration was significantly different (P<0.0001) at 24 hours postoperatively between groups. There was no significant difference in haptoglobin concentration between groups. C-reactive protein and haptoglobin concentrations were significantly different at 24 and 48 hours postoperatively for minimally invasive and conventional ovariohisterectomies. These findings provided an overview of the short-term inflammatory effects produced by minimally invasive and conventional ovariohysterectomies.

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