Simultaneous measurement of two major prostacyclin metabolites in urine

1990 ◽  
Vol 36 (11) ◽  
pp. 1978-1980 ◽  
Author(s):  
S Zureick ◽  
J Nadler ◽  
J Yamamoto ◽  
R Horton
Keyword(s):  
Angiotensin Ii ◽  
Blood Vessels ◽  
Simultaneous Measurement ◽  
Healthy Subjects ◽  
High Accuracy ◽  
High Concentration ◽  
Accuracy And Precision ◽  
Overall Evaluation ◽  
Excretion Rates ◽  
Apparently Healthy

Abstract We describe a combined HPLC-RIA technique to measure both major metabolites of prostacyclin (PGI2): 6-keto PGF1 alpha and 2,3-dinor-6 keto PGF1 alpha. The measurement of the former, which originates from renal blood vessels, and the latter, from systemic vessels and the liver, may provide a better overall evaluation of production than measurement of one metabolite. An aliquot of acidified urine with added 3H-labeled metabolites is adsorbed and then eluted from a C18 Bond-Elut column. The sample is then passed through an HPLC system by use of an isocratic solvent combination that separates the two metabolites from known prostaglandins. The purified metabolites are then quantified by RIA. Using a logit-log10 transform, one can measure between 12 and 250 pg of either metabolite, with high accuracy and precision (CVs of 12% for a low concentration and 7% for a high concentration). Reference values for apparently healthy subjects were, respectively, 107 (SD 45) and 171 (SD 69) ng/g creatinine for 6-keto PGF1 alpha and the dinor metabolite in men (n = 18) and 45 (SD 22) and 141 (SD 28) ng/g creatinine, respectively, in women (n = 15). Indomethacin in standard doses reduced both metabolite values by 50%. Intravenous administration of angiotensin II (5 ng/kg of body wt per minute) did not alter excretion rates, but equipressor doses of norepinephrine (0.1 microgram/kg per minute) increased the production of both metabolites (6-keto greater than dinor).

scholarly journals Faster 3D saturation-recovery based myocardial T1 mapping using a reduced number of saturation points and denoising

2019 ◽  
Author(s):  
Giovanna Nordio ◽  
Aurélien Bustin ◽  
Freddy Odille ◽  
Torben Schneider ◽  
Markus Henningsson ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Statistical Difference ◽  
T1 Mapping ◽  
Free Breathing ◽  
High Accuracy ◽  
Saturation Recovery ◽  
Accuracy And Precision ◽  
Before And After ◽  
Myocardial T1

AbstractPurposeTo accelerate the acquisition of free-breathing 3D saturation-recovery-based (SASHA) myocardial T1 mapping by acquiring fewer saturation points in combination with a novel post-processing 3D denoising technique to maintain high accuracy and precision.Methods3D SASHA T1 mapping acquires nine T1-weighted images along the saturation recovery curve, resulting in long acquisition times. In this work, we propose to accelerate conventional cardiac T1 mapping by reducing the number of saturation points. High T1 accuracy and precision is maintained by applying a 3D denoising technique to the T1-weighted images prior to pixel-wise T1 fitting. The proposed approach was evaluated on a T1 phantom and 20 healthy subjects, by varying the number of T1-weighted images acquired between three and nine, both prospectively and retrospectively. Three patients with suspected cardiovascular disease were acquired using five T1-weighted images. T1 accuracy and precision was determined for all the acquisitions before and after denoising.ResultsIn the T1 phantom, no statistical difference was found in terms of accuracy and precision for the different number of T1-weighted images before or after denoising (P=0.99 and P=0.99 for accuracy, P=0.64 and P=0.42 for precision, respectively). In vivo, both prospectively and retrospectively, the precision improved considerably with the number of T1-weighted images employed before denoising (P<0.05) but was independent on the number of T1-weighted images after denoising.ConclusionWe demonstrate the feasibility of accelerating 3D SASHA T1 mapping by reducing the number of acquired T1-weighted images in combination with an efficient 3D denoising, without affecting accuracy and precision of T1 values.

Concentrations of non-glucose polyols in serum and cerebrospinal fluid from apparently healthy adults and children.

1984 ◽  
Vol 30 (2) ◽  
pp. 188-191 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
S Seki ◽  
K Seki
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Healthy Subjects ◽  
Metabolic Diseases ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Adults And Children ◽  
Healthy Persons ◽  
Apparently Healthy

Abstract Six non-glucose polyols--mannose, fructose, 1-deoxyglucose, mannitol, glucitol, and inositol--were identified and evaluated in human serum and cerebrospinal fluid by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Concentrations of fructose, mannose, and inositol in the serum of healthy persons or children without metabolic diseases varied with age, as already reported for 1-deoxyglucose. Fructose, inositol, and glucitol concentrations in cerebrospinal fluid significantly exceeded those in serum. The method described here for determining polyols and for evaluating polyol patterns in serum, as well as the resulting data on children and healthy subjects, should be useful in investigations of the clinical and physiological significance of polyols.

scholarly journals Exercise-Induced Release of Cardiac Troponins in Adolescent vs. Adult Swimmers

2021 ◽  
Vol 18 (3) ◽  
pp. 1285
Author(s):  
Rafel Cirer-Sastre ◽  
Francisco Corbi ◽  
Isaac López-Laval ◽  
Luis Enrique Carranza-García ◽  
Joaquín Reverter-Masià
Keyword(s):  
Cardiac Troponin ◽  
Healthy Subjects ◽  
Troponin T ◽  
Training Experience ◽  
Post Exercise ◽  
Baseline Values ◽  
Exercise Induced ◽  
Cardiac Troponins ◽  
Similar Peak ◽  
Apparently Healthy

To examine the exercise-induced release of cardiac troponin T (cTnT) in adolescent and adult swimmers. Thirty-two trained male (18 adolescents, 14 adults) swam at maximal pace in a 45 min distance trial, and blood samples were drawn before, immediately and 3 h after exercise for subsequent cTnT analysis and comparison. Having comparable training experience and baseline values of cTnT (p = 0.78 and p = 0.13), adults exercised at lower absolute and relative intensity (p < 0.001 and p < 0.001, respectively), but presented higher immediate cTnT after exercise than adolescents (p < 0.001). Despite that, peak concentrations were observed at 3 h post exercise and peak elevations were comparable between groups (p = 0.074). Fourteen (44%) apparently healthy subjects exceeded the cutoff value for myocardial infarction (MI). Adolescents presented a delayed elevation of cTnT compared with adults, but achieved similar peak values.

scholarly journals POS0169 FETUIN-A AS A MARKER OF OSTEOPOROSIS AND OSTEOPOROTIC FRACTURES IN PATIENTS WITH RHEUMATOID ARTHRITIS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 297.2-297
Author(s):  
Y. Akhverdyan ◽  
E. Papichev ◽  
В. Zavodovsky ◽  
L. Seewordova ◽  
J. Polyakova
Keyword(s):  
Rheumatoid Arthritis ◽  
Blood Serum ◽  
Bone Mineralization ◽  
Expectant Management ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
High Concentration ◽  
Mineral Density ◽  
Fetuin A ◽  
Apparently Healthy

Background:The main mechanism of the effect of fetuin-A (FeA) on bone metabolism is its ability to bind calcium and proteins of the TGF-β family. It has been proven that the optimal concentration of TGF-β is necessary for the differentiation of bone tissue, and a high concentration inhibits bone mineralization. Thus, adequate osteogenesis is based on a complex balance between FeA and TGF-β levels. It can be assumed that the determination of the FeA level in the blood of patients with rheumatoid arthritis (RA) will help to optimize the diagnosis and predict the severity of osteoporosis (OP).Objectives:to study the possibility of predicting the development of osteoporosis and osteoporetic fractures in patients with RA, depending on the level of FeA in blood serum.Methods:We examined two groups of patients (52 patients with RA complicated by OP, 58 patients with RA without OP) and 30 apparently healthy individuals. The age of the surveyed ranged from 18 to 72 years, the average duration of the disease was 7.53±0.89 years. In both groups, the FeA level was determined by an indirect enzyme-linked immunosorbent assay using a commercial test. Bone mineral density (BMD) was also measured in both groups (Lunar DPX-NT GE).Results:The average FeA level in the group of RA patients was lower than in the group of conventionally healthy individuals (731.21±109.9 μg/ml and 812.9±76.2 μg/ml, respectively; F=13.34; p=0,0004). The normal FeA level was calculated using the formula M±2σ in the group of apparently healthy individuals and ranged from 653.55 μg/ml to 972.19 μg/ml.A decreased level of FeA was found in 20 patients (86.96%) in the group of patients with OP and only in 3 (13.04%) patients with RA who did not suffer from OP (p<0.001). It can be concluded that patients with RA and a low concentration of FeA in the blood serum have a higher risk of developing OP.In the group of patients with normal FeA level, osteoporetic fractures were observed in 12 (13.79%) patients and were absent in 75 (86.21%) patients (p<0.001). Thus, RA patients with normal serum FeA levels have a lower risk of osteoporetic fractures.We also found a positive significant correlation between the level of FeA and BMD in the femoral neck area. In the group of patients with a reduced FeA level (23 people), the mean BMD values were 0.732±0.022 g/cm2, and in the group of patients with a normal FeA level (87 patients) - 0.890±0.014 g/cm2 (p<0.001, F=27.663). The obtained values are in agreement with the literature data on the effect of the serum FeA concentration on the BMD values.Conclusion:We consider it expedient to determine the serum FeA concentration in patients with RA. At a FeA level of 653.55 μg/ml and below, a higher risk of developing OP and osteoporetic fractures can be predicted. In this case, the patient is shown a standard examination for osteoporosis. At values of 653.55 μg/ml and above, a more expectant management of the patient is allowed. Thus, by determining the serum concentration of FeA, it is possible to implement an integrated approach to the patient and to optimize the schemes for the diagnosis of OP in patients with RA.Disclosure of Interests:None declared

scholarly journals Impaired Cardiac Autonomic Functions in Apparently Healthy Subjects with Vitamin D Deficiency

2014 ◽  
Vol 20 (4) ◽  
pp. 378-385 ◽  
Author(s):  
Uğur Canpolat ◽  
Fırat Özcan ◽  
Özcan Özeke ◽  
Osman Turak ◽  
Çağr Yayla ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Healthy Subjects ◽  
Autonomic Functions ◽  
Apparently Healthy

scholarly journals UPLC-MS/MS Determination of Linezolid and Heme in Plasma of Infected Patients and Correlation Analysis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yingying Wang ◽  
Xuemei Ye ◽  
Qin Lan ◽  
Xiaofang Ke ◽  
Lufeng Hu ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Healthy Subjects ◽  
Haematological Toxicity ◽  
Diagnostic Value ◽  
High Concentration ◽  
High Positive Correlation ◽  
Positive Correlation ◽  
Early Diagnose ◽  
The Relationship

Linezolid can cause serious haematological toxicity, such as thrombocytopenia and aneamia. Heme, composed of iron and porphyrin, is an important component of hemoglobin. In order to investigate the relationship between the concentration of linezolid and heme in the plasma of infected patients, a UPLC-MS/MS method that can determine the concentrations of linezolid and heme simultaneously was developed and validated. A total of 96 healthy subjects and 81 infected patients, who received blood routine blood tests, were included and determined by the UPLC-MS/MS method. The results showed that the concentration of linezolid was 5.08 ± 3.46   μ g / mL in infected patients who were treated with linezolid. The heme in healthy subjects was 7.05 ± 8.68   μ g / mL , and it was significantly decreased to 0.88 ± 0.79   μ g / mL in infected patients ( P < 0.01 ). Spearman correlation analysis showed that linezolid had a high negative correlation with platelet (PLT) ( R = − 0.309 ). Heme had a high positive correlation with hemoglobin (Hb) ( R = 0.249 ) in healthy subjects and infected patients. The ROC analysis showed that heme had diagnostic value to distinguish low Hb (110 g/L). In conclusion, there was a positive correlation between heme and Hb, and this correlation was also observed in infected patients. A high concentration of linezolid was inclined to decrease PLT. Monitoring of heme and linezolid helps in the early diagnose of low Hb and PLT.

scholarly journals Dysfunctional Adiposity Index as a Useful Clinical Tool for Early Identification of Adipose Tissue Morpho-Functional Abnormalities and Cardiometabolic Disorders in Apparently Healthy Subjects

2020 ◽  
Author(s):  
Juan Reyes-Barrera ◽  
Victor H. Sainz-Escárrega ◽  
Aida X. Medina-Urritia ◽  
Esteban Jorge-Galarza ◽  
Horacio Osorio-Alonso ◽  
...  
Keyword(s):  
Risk Factors ◽  
Adipose Tissue ◽  
Clinical Practice ◽  
Early Identification ◽  
Healthy Subjects ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Adiposity Index ◽  
Apparently Healthy

Abstract BackgroundCompared to body mass index (BMI), waist circumference (WC), and adiposity measurements, adipose tissue morpho-functionality evaluations are more consistent predictors of cardiometabolic abnormalities. However, these evaluations require determination of adipokines and other non-routine biochemical parameters, which is not feasible in clinical practice. The present study establishes dysfunctional adiposity index (DAI) as a simple, accessible, and reliable marker of early adipocytes morpho-functional abnormalities and cardiometabolic diseases.MethodsTo establish the DAI constant parameters, 340 subjects (134 males and 206 females) without cardiovascular risk factors were selected from a cross-sectional study. Then, DAI was calculated in 36 healthy subjects who underwent subcutaneous adipose tissue biopsy, for whom adipocytes number and size, body composition, circulating adipokines, glucose, insulin, and lipids were also determined. The correlation of DAI with adipocyte morphology (size/number of adipocytes) and functionality (adiponectin/leptin ratio) was analyzed. The receiver operating characteristic curve was used to define the optimal DAI cut-off point to identify metabolic abnormalities. Finally, the independent association of DAI with cardiometabolic abnormalities was determined in 1418 subjects from the cross-sectional study through multivariate analyses.ResultsThe constant parameters to calculate the DAI were [WC/[22.79+[2.68*BMI]]]*[triglycerides (TG, mmol/L)/1.37]*[1.19/high density lipoprotein-cholesterol (HDL-C, mmol/L)] for males, and [WC/[24.02+[2.37*BMI]]]*[TG(mmol/L)/1.32]*[1.43/HDL-C(mmol/L)] for females. In subjects underwent biopsy, DAI correlated with adipocytes mean area (r=0.358; p=0.032), adipocyte number (r=-0.381; p=0.024), adiponectin/leptin ratio (r=-0.483; p=0.003), and systemic inflammation markers. Compared to BMI, WC, and visceral fat, DAI was the only determination associated with insulin resistance (area under the curve: 0.743; p = 0.017). In the cross-sectional study, DAI ≥1.065 was independently associated with diabetes (OR: 1.96; 95%CI: 1.36-2.84), non-alcoholic fatty liver disease (OR: 2.57; 95%CI: 1.98-3.33), subclinical atherosclerosis (OR: 1.74; 95%CI: 1.02-2.94), and hypertension (OR: 1.44; 95%CI: 1.10-1.88).ConclusionsThe present study establishes the constant parameters to calculate the DAI and highlights that a DAI ≥ 1.065 is associated with early cardiometabolic abnormalities independently of adiposity and other risk factors. Since DAI is calculated using accessible parameters routinely used in the clinic, this indicator can be easily incorporated in clinical practice for the early identification of adipose tissue abnormalities in apparently healthy subjects.

scholarly journals MULTI-CHANNEL FRY COUNTER DESIGN USING OPTOCOUPLER SENSOR

2019 ◽  
Vol 4 (2) ◽  
pp. 97-104
Author(s):  
Lazuardi Umar ◽  
Yanuar Hamzah ◽  
Rahmondia N. Setiadi
Keyword(s):  
Counting Process ◽  
Production Capacity ◽  
High Accuracy ◽  
Limited Capacity ◽  
High Concentration ◽  
Counting System ◽  
Channel Detection ◽  
Accuracy Level ◽  
High Level ◽  
Almost All

This paper describes a design of a fry counter intended to be used by consuming fish farmer. Along this time, almost all the fry counting process is counted by manual, which is done by a human. It is requiring much energy and needs high concentration; thus, can cause a high level of exhaustion for the fry counting worker. Besides that, the human capability and capacity of counting are limited to a low level. A fry counter design in this study utilizes a multi-channel optocoupler sensor to increase the counting capacity. The multi-channel fry counter counting system is developed as a solution to a limited capacity of available fry counter. This design uses an input signal extender system on controller including the interrupt system. From the experiment, high accuracy level is obtained on the counting and channel detection, therefore, this design can be implemented and could help farmers to increase the production capacity of consuming fish.

scholarly journals The Relationship Between Hyperglycemia and the Rheumatoid Factor in the Serum of Diabetic Patients

2009 ◽  
Vol 6 (1) ◽  
pp. 99-105
Author(s):  
Baghdad Science Journal
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Healthy Subjects ◽  
Diabetic Patients ◽  
Diabetic Mellitus ◽  
Positive Rheumatoid Factor ◽  
Common Diseases ◽  
Males And Females ◽  
The Relationship ◽  
Apparently Healthy

This study was conducted to determine the relationship between two most common diseases in Iraqis patients , which are Diabetic mellitus (DM) and Rheumatoid Arthritis (RA); seeking rheumatoid factor in hyperglycemic sera. The results revealed that ; 62.5% of hyperglycemic (HG) patients had positive rheumatoid factor (RF) . No difference in number between both gender of HG patients (20 males and 20 females ) , RF reaction was nearly similar in males and females of HG patients ( 12 &13 respectively ) . Only 40% out of patient controls had positive RF . None of the apparently healthy subjects had positive RF .

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