P25 DETECTION OF CIRCULATING TUMOR CELLS IN POTENTIALLY RESECTABLE ADENOCARCINOMA OF DE DISTAL ESOPHAGUS AND THE GASTRO-ESOPHAGEAL JUNCTION DOES NOT INCREASE IN PORTAL VENOUS BLOOD SAMPLES COMPARED TO PERIPHERAL VENOUS BLOOD SAMPLES

2019 ◽  
Vol 32 (Supplement_2) ◽  
Author(s):  
Depypere Lieven ◽  
Philippron Annouck ◽  
De Preter Katleen ◽  
Coosemans Willy ◽  
Van Veer Hans ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Detection Rate ◽  
Venous Blood ◽  
Distal Esophagus ◽  
Intact Cells ◽  
Abdominal Lymph Node ◽  
Blood Samples ◽  
Peripheral Venous Blood ◽  
Portal Venous Blood

Abstract Aim To compare the number of circulating tumor cells (CTCs) in portal venous blood samples of patients with potentially resectable adenocarcinoma of de distal esophagus and the gastro-esophageal junction (EAC) with the number of CTCs in peripheral venous blood samples. Background and Methods Detection of (CTCs) in potentially resectable (EAC) is rare in peripheral venous blood samples(1). In lung carcinoma patients, the number of circulating tumor cells was more than 300 fold in the pulmonary vein, compared to the number in peripheral venous blood samples (2). In patients undergoing esophagectomy for cancer, peripheral blood was sampled immediately preoperatively and portal vein blood was sampled intraoperatively during abdominal lymph node dissection. Samples were analyzed for CTCs using the parsortix device (ANGLE): a semi-automated microfluidic system that captures cells based on their size and rigidity. A four-color immunofluorescence technique was used and CK positive, CD45 negative, Hoechst positive and morphologically intact cells with the morphology of a CTC were counted manually. The method was previously compared with the commercially available Cellseach® system and no difference in CTC yield between both methods. Results 20 patients with potentially resectable EAC were evaluated. One peripheral venous sample and two portal venous samples were not applicable. In 5 out of 19 peripheral venous samples (26,3%), one or more CTC’s could be detected, while in this was only the case in 3 out of 18 portal venous samples (16.7%). Conclusions The number of detected CTCs in potentially resectable EAC was not increased in portal venous samples compared to peripheral venous samples. Further research is needed on why detection rate of CTCs in potentially resectable EAC is so low and how detection rate could be increased.

scholarly journals No evidence for shedding of circulating tumor cells to the peripheral venous blood as a result of mammographic breast compression

2013 ◽  
Vol 141 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Daniel Förnvik ◽  
Ingvar Andersson ◽  
Magnus Dustler ◽  
Roy Ehrnström ◽  
Lisa Rydén ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Venous Blood ◽  
Peripheral Venous Blood ◽  
Breast Compression

Detection of circulating tumor cells (CTCs) in central venous blood (CVB) versus peripheral venous blood (PVB) in patients with metastatic cancers.

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. e22039-e22039 ◽  
Author(s):  
Ayhan Ulusakarya ◽  
Fei Ye ◽  
Janine Wechsler ◽  
Pasquale F Innominato ◽  
Mazen Haydar ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Venous Blood ◽  
Central Venous ◽  
Peripheral Venous Blood ◽  
Central Venous Blood

Enumeration of circulating tumor cells and assessment of immunotherapy biomarker PD-L1 in patients with colorectal cancer.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e23029-e23029
Author(s):  
Ju-Yu Tseng ◽  
Chwen Cheng Chen ◽  
Chun-CHI Lin ◽  
Hung-Hsin Lin ◽  
Yen-Ru Chen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Detection Rate ◽  
Venous Blood ◽  
Clinical Manifestations ◽  
Operative Management ◽  
Therapeutic Monitoring ◽  
Clinical Sensitivity ◽  
Preoperative Serum

e23029 Background: Circulating tumor cells (CTC) play a prognostic role in patients with metastatic colorectal cancer (mCRC). Studies investigating detection of CTC in peripheral blood (PB) showed limited clinical sensitivity. This study compares clinical sensitivity of CTC analysis in mesenteric venous blood (MVB) vs PB. PD-L1 expression on isolated CTCs was also characterized in various stages of CRC patients. Methods: 8mL PB and MVB samples were collected into K2EDTA-containing vacationer tubes and subsequently performed CTC enumeration and PD-L1 analysis by MiSelect R system. CTC is defined as EpCAM+, cytokeratin+ and CD45- with intact nuclei. CTC and PD-L1+ CTC number were counted and correlated to the patients’ clinical manifestations. Results: Blood samples were collected from 75 CRC patients with various stages. In MVB, CTC detection rate was 20%, 33%, 44% and 58% for stage I, II, III, and IV respectively, and the overall detection rate is 41%. In PB, CTC only detected in sporadic cases (7%). Also, the amount of CTC detected in MVB was more abundant than that in PB ( P < 0.001) In addition, preoperative serum CEA levels were significantly associated with presence of CTC in MVB ( P= 0.011). PD-L1 expression was detected in 32% of the isolated CTCs, and was more abundant in those from late stage patients. The expression level of PD-L1 showed heterogeneity among CTCs. Conclusions: CTC enumeration in MVB could potentially increase the clinical sensitivity for CTC analysis in CRC. The CTC level in MVB significantly correlated with CEA levels which could serve as an important biomarker information for post-operative management. In additions, using MiSelect R System to determine PD-L1 expression on CTC could provide a promising approach for both patient selection or therapeutic monitoring of immunotherapies for CRC.

scholarly journals Comparison of capillary, venous and buffy coat blood samples in detecting Plasmodium species among malaria suspected patients attending at Hamusite health center. A cross-sectional study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Getu Abeje ◽  
Woyneshet Gelaye ◽  
Getaneh Alemu
Keyword(s):  
Health Center ◽  
Detection Rate ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Buffy Coat ◽  
Blood Film ◽  
Capillary Blood ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples

Abstract Background Both capillary and venous blood samples have been interchangeably used for the diagnosis of malaria in Ethiopia. However, Plasmodium parasites are thought to be more concentrated in capillary than in venous blood. Hence, selecting a sample source where parasites are more concentrated is indispensable approach in order to maximize the accuracy of blood film microscopy. Therefore, the present study aimed to compare the detection rate and the parasitemia level of Plasmodium species from conventional capillary and venous blood films, and buffy coat preparations. Methods A facility based cross-sectional study was conducted from Feburary to March 2020 among 210 febrile patients attending Hamusite health center, northwest Ethiopia. Capillary and venous blood samples were collected and buffy coat was prepared from each sample. Thin and thick blood films were prepared, stained, and examined microscopically following standard protocol. Data were analysed using Statistical Package for Social Sciences Software version 20 and Med-Calc software version 19.3. Results Capillary blood buffy coat (61/210, 29.0%) had significantly higher detection rate as compared to capillary (48/210, 22.9%) and venous (42/210, 20.0%) blood films (p < 0.001). However, no significant difference was observed between capillary and venous blood films (p = 0.070) in detecting Plasmodium species. The highest and the lowest mean asexual stage parasite counts were found in capillary blood buffy coat (4692.88) and venous blood (631.43) films, respectively showing significant variations (p < 0.001). Mean gametocyte count was also highest in capillary blood buffy coat (3958.44). As compared to capillary blood buffy coat, the sensitivity of venous blood buffy coat, capillary blood film and venous blood film were 73.8, 78.7, 68.9%, respectively. Conclusion Capillary blood buffy coat samples showed the highest sensitivity in detecting and quantitating malaria parasites that its use should be promoted in clinical settings. However, conventional capillary and venous blood films could be used interchangeably.

scholarly journals A Modified Method to Isolate Circulating Tumor Cells and Identify by a Panel of Gene Mutations in Lung Cancer

2021 ◽  
Vol 20 ◽  
pp. 153303382199527
Author(s):  
Helin Wang ◽  
Jieqing Wu ◽  
Qi Zhang ◽  
Jianqing Hao ◽  
Ying Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Detection Rate ◽  
Target Genes ◽  
White Blood Cells ◽  
Gene Mutations ◽  
Copy Number Variations ◽  
Immunomagnetic Beads ◽  
Membrane Rupture

The CellSearch system is the only FDA approved and successful used detection technology for circulating tumor cells(CTCs). However, the process for identification of CTCs by CellSearch appear to damage the cells, which may adversely affects subsequent molecular biology assays. We aimed to explore and establish a membrane-preserving method for immunofluorescence identification of CTCs that keeping the isolated cells intact. 98 patients with lung cancer were enrolled, and the efficacy of clinical detection of CTCs was examined. Based on the CellSearch principle, we optimized an anti-EpCAM antibody and improved cell membrane rupture. A 5 ml peripheral blood sample was used to enrich CTCs with EpCAM immunomagnetic beads. Fluorescence signals were amplified with secondary antibodies against anti-EpCAM antibody attached on immunomagnetic beads. After identifying CTCs, single CTCs were isolated by micromanipulation. To confirm CTCs, genomic DNA was extracted and amplified at the single cell level to sequence 72 target genes of lung cancer and analyze the mutation copy number variations (CNVs) and gene mutations. A goat anti-mouse polyclonal antibody conjugated with Dylight 488 was selected to stain tumor cells. We identified CTCs based on EpCAM+ and CD45+ cells to exclude white blood cells. In the 98 lung cancer patients, the detection rate of CTCs (≥1 CTC) per 5 ml blood was 87.76%, the number of detections was 1–36, and the median was 2. By sequencing 72 lung cancer-associated genes, we found a high level of CNVs and gene mutations characteristic of tumor cells. We established a new CTCs staining scheme that significantly improves the detection rate and allows further analysis of CTCs characteristics at the genetic level.

scholarly journals Significance of Circulating Tumor Cells Detected by the CellSearch System in Patients with Metastatic Breast Colorectal and Prostate Cancer

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
M. Craig Miller ◽  
Gerald V. Doyle ◽  
Leon W. M. M. Terstappen
Keyword(s):  
Prostate Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Treatment Options ◽  
Venous Blood ◽  
Metastatic Breast ◽  
Cellsearch System ◽  
Multicenter Studies ◽  
Real Time Analysis ◽  
Number Of Treatment

The increasing number of treatment options for patients with metastatic carcinomas has created a concomitant need for new methods to monitor their use. Ideally, these modalities would be noninvasive, be independent of treatment, and provide quantitative real-time analysis of tumor activity in a variety of carcinomas. Assessment of circulating tumor cells (CTCs) shed into the blood during metastasis may satisfy this need. We developed the CellSearch System to enumerate CTC from 7.5 mL of venous blood. In this review we compare the outcomes from three prospective multicenter studies investigating the use of CTC to monitor patients undergoing treatment for metastatic breast (MBC), colorectal (MCRC), or prostate cancer (MPC) and review the CTC definition used in these studies. Evaluation of CTC at anytime during the course of disease allows assessment of patient prognosis and is predictive of overall survival.

scholarly journals Activated T Cells and Soluble Molecules in the Portal Venous Blood of Patients with Cholestatic and Hepatitis C Virus-Positive Liver Cirrhosis. Possible Promotion of Fas/FasL-Mediated Apoptosis in the Bile-Duct Cells and Hepatocyte Injury

2003 ◽  
Vol 31 (3) ◽  
pp. 170-180 ◽  
Author(s):  
N Ariki ◽  
Y Morimoto ◽  
T Yagi ◽  
T Oyama ◽  
Y Cyouda ◽  
...  
Keyword(s):  
T Cells ◽  
Liver Cirrhosis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Bile Duct ◽  
Venous Blood ◽  
Activated T Cells ◽  
Peripheral Venous Blood ◽  
Nk T Cells ◽  
Portal Venous Blood

We investigated the immune responses of patients with cholestatic and hepatitis C virus-positive (HCV-positive) liver cirrhosis by analysing T-cell subsets and cytokine levels in the portal and peripheral veins, using flow cytometry and enzyme-linked immunosorbent assay. In cholestatic liver cirrhosis, the proportion of natural-killer (NK) T cells and interleukin (IL) 6 and IL-18 levels in the portal venous blood were significantly higher than those in the peripheral venous blood. In HCV-positive liver cirrhosis, the proportions of NK T cells and Fas+ T cells and IL-6 and soluble Fas levels in the portal venous blood were significantly higher than those in the peripheral venous blood. These results suggest that in these diseases, activated T cells and soluble molecules in portal venous blood may promote Fas/FasL-mediated apoptosis of the bile-duct cells and hepatocytes, and contribute to the deterioration in liver function as an inevitable result of positive feedback.

Direct Measurement of the Hepatointestinal Extraction of Zinc in Cirrhosis and Hepatitis

1981 ◽  
Vol 61 (4) ◽  
pp. 441-444 ◽  
Author(s):  
P. W. N. Keeling ◽  
W. Ruse ◽  
J. Bull ◽  
B. Hannigan ◽  
R. P. H. Thompson
Keyword(s):  
Chronic Hepatitis ◽  
Statistical Significance ◽  
Venous Blood ◽  
Zinc Content ◽  
Atomic Absorption Spectrophotometry ◽  
Zinc Extraction ◽  
Blood Samples ◽  
Peripheral Venous Blood ◽  
Absorption Spectrophotometry ◽  
Biopsy Specimens

1. 65Zn was injected intravenously during transjugular liver biopsy and, from simultaneous hepatic and peripheral venous blood samples, hepatointestinal 65Zn extraction was calculated. Hepatic zinc content was measured in biopsy specimens. 2. On the same occasion samples of liver tissue were taken and their zinc content was measured by atomic absorption spectrophotometry. 3. Seven patients with cirrhosis had significantly lower hepatic zinc content and hepatointestinal zinc extraction than six control patients with mild liver disease. Six patients with chronic hepatitis had a mean hepatointestinal zinc extraction higher than control patients, whereas their mean hepatic zinc content was lower, although the former difference did not achieve statistical significance. 4. These results demonstrate that hepatointestinal extraction of zinc is impaired in cirrhosis, but not in chronic hepatitis.

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