ABSTRACT
Diurnal concentrations of serum melatonin were determined longitudinally in female rhesus monkeys throughout sexual maturation to ascertain how levels varied with advancing age and reproductive onset. Females were housed either in outdoor enclosures (n = 8) exposed to ambient environmental conditions, or indoors (n = 4) under a photoperiod of 12 h light: 12 h darkness and fixed temperature of 20–23 °C. Animals were studied from immaturity (15 months) through first ovulation and were additionally compared with fully adult female rhesus monkeys (n = 5) studied during the annual breeding season. The diurnal melatonin pattern was described for the developing females in the summer, autumn and winter in 3 successive years from samples collected at 10.00, 18.00, 22.00, 02.00, 06.00 and 10.00 h.
Nocturnal levels of melatonin declined significantly during development in both indoor- and outdoor-housed females with a progressive decrease up to 33 months of age. Daytime values were consistently low but exhibited a slight decline also with age. Nocturnal values in all months sampled fell significantly with greater decreases occurring at the earliest ages. Furthermore, superimposed upon this developmental change, animals housed outdoors responded to seasonal changes in photoperiod with diurnal increases in melatonin occurring after sunset. The females in the present study exhibited first ovulation at two distinct ages: 32–37 months ('early', n = 6) and 41–45 months ('later', n=5). One female did not ovulate within the study period. Although nocturnal levels of serum melatonin were similar between the two groups up to 29 months of age, a post-hoc analysis revealed that concentrations were significantly lower by 34 months of age for the early group, a time coincident with first ovulation. Nocturnal levels of melatonin remained high, relative to the early group, in the later ovulating females until 43 months of age, coincident with first ovulation for these animals. The diurnal pattern of serum melatonin at first ovulation, regardless of chronological age, was similar to that observed during the ovulatory season for adult female rhesus monkeys.
These data suggest that nocturnal melatonin concentrations decline with advancing chronological age in prepubertal female rhesus monkeys. Furthermore, the timing of sexual maturation was inversely related to nocturnal melatonin. Whether this change in melatonin is causally related to reproductive onset or, rather, is a consequence of other factors regulating the occurrence of first ovulation remains to be determined. Furthermore, the observation that the melatonin rhythm in outdoor-housed females follows the prevailing photoperiod permits the hypothesis that this rhythm may mediate any photoperiodic effect on the seasonal occurrence of first ovulation characteristic of rhesus monkeys housed outdoors.
Journal of Endocrinology (1989) 121, 553–562
Early prenatal androgenization results in diminished ovarian reserve in adult female rhesus monkeys