scholarly journals Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry

2005 ◽  
Vol 56 (2) ◽  
pp. 331-336 ◽  
Author(s):  
Nuno Cerca ◽  
Silvia Martins ◽  
Filipe Cerca ◽  
Kimberly K. Jefferson ◽  
Gerald B. Pier ◽  
...  
2017 ◽  
Vol 15 (2) ◽  
pp. 124-129 ◽  
Author(s):  
Rabina Basnet ◽  
Sudarshan Sharma ◽  
Jid Chani Rana ◽  
Pradeep Kumar Shah

Background: Otitis Media is a prevailing and notorious infection in developing countries causing serious local damage and threating complication. Mainly in developing countries like Nepal, Otitis Media results because of illiteracy, poverty and poor hygiene. The aim of this study was to determine the profile of Otitis Media, its causative agents and their antibiotic susceptibility pattern.Methods: The study included 263 pus samples from 240 patients attending ENT department of Bharatpur hospital from May 2015 to January 2016. Samples were processed in microbiology department for bacteria using standard operating protocol. Antibiotic susceptibility testing was performed for all bacterial isolates by Kirby Bauer disc diffusion method and the results were interpreted according to clinical and laboratory standard institute (CLSI) guideline.Results: Out of 240 patients, 121 were female and 119 were male. Highest incidence of Otitis Media was observed in 1-10 year age group. Out of 263 samples taken from 240 patients, 216 showed bacterial growth. Gram negative bacteria predominated and the most common bacteria isolated were Staphylococcus aureus 36.11% followed by Pseudomonas aeruginosa 33.33% and Coagulase Negative Staphylococci 8.08%. All bacterial isolates were sensitive to gentamycin. Staphylococcus aureus was sensitive to Amikacin and gentamycin. All gram negative bacterial isolates were sensitive to Imipenem and gentamycin. 100% of Pseudomonas aeruginosa was sensitive to Imipenem.Conclusions: Staphylococcus aureus was the most predominant organism isolated from the pus swab followed by Pseudomonas aeruginosa and all the isolated organisms were sensitive to Gentamycin.


1999 ◽  
Vol 83 (7) ◽  
pp. 771-773 ◽  
Author(s):  
A. Pinna ◽  
S. Zanetti ◽  
M. Sotgiu ◽  
L. A Sechi ◽  
G. Fadda ◽  
...  

2004 ◽  
Vol 23 (6) ◽  
pp. 637-640 ◽  
Author(s):  
Paolo Moroni ◽  
Francesca Vellere ◽  
Micaela Antonini ◽  
Giuliano Pisoni ◽  
Gianfranco Ruffo ◽  
...  

2014 ◽  
Vol 8 (12) ◽  
pp. 1643-1645 ◽  
Author(s):  
Olayemi Oluseun Ayepola ◽  
Nurudeen Ayoade Olasupo ◽  
Louis Osayenum Egwari ◽  
Frieder Schaumburg

This item has no abstract. Follow the links below to access the full text.


2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Shamshul Ansari ◽  
Hari Prasad Nepal ◽  
Rajendra Gautam ◽  
Sony Shrestha ◽  
Puja Neopane ◽  
...  

Introduction. Children are among the most vulnerable population groups to contract illnesses. The varying microbiological pattern of septicemia warrants the need for an ongoing review of the causative organisms and their antimicrobial susceptibility pattern. Therefore, the objective of this study was to document the bacterial etiology of childhood septicemia and its antibiotic susceptibility profile.Methods.Cross-sectional type of study in 1630 suspected patients was conducted at CMCTH from January 2012 to December 2013. Blood samples were collected aseptically for culture. The organisms grown were identified by standard microbiological methods recommended by American Society for Microbiology (ASM) and subjected to antibiotic susceptibility testing by modified Kirby-Bauer disk diffusion method. Methicillin resistance was confirmed using cefoxitin and oxacillin disks methods.Results.Septicemia was detected in 172 (10.6%) cases. Among Gram-positive organisms, coagulase negative staphylococci (CoNS) were leading pathogen andAcinetobacterspp. were leading pathogen among Gram-negative isolates. Vancomycin, teicoplanin, and clindamycin were the most effective antibiotics against Gram-positive isolates while amikacin was effective against Gram-positive as well as Gram-negative isolates. Methicillin resistance was detected in 44.4% ofStaphylococcus aureus.Conclusions. This study has highlighted the burden of bacterial etiology for septicemia among children in a tertiary care center of central Nepal.


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