Colorimetric and Gas-Liquid Chromatographic Determination of Atropine-Hyoscyamine and Hyoscine (Scopolamine) in Solanaceous Plants
Keyword(s):
Abstract Two colorimetric micromethods are described for the determination of atropine-hyoscyamine and hyoscine (scopolamine), using p-dimethylaminobenzaldehyde and citric acid-acetic anhydride as the color reagents. These methods are sensitive to 60-1200 and 10-360 μg alkaloid/10 ml. The colorimetric methods were also successfully applied after a preliminary thin layer chromatographic separation of the alkaloids. A gasliquid chromatographic procedure was also developed, which yielded comparable results with the colorimetric methods.
2012 ◽
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1985 ◽
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