Liquid Chromatographic Determination of Glucosinolates in Rapeseed as Desulfoglucosinolates
Keyword(s):
Abstract A method was developed for the quantitative determination of rapeseed glucosinolates as the desulfo derivatives, using liquid chromatography. Glucosinolates were desulfated at 37°C with the enzyme aryl sulfatase in Tris buffer, pH 8.0. All glucosinolates present in rapeseed were separated in 30 min on a Waters C18 Z-module using an acetonitrile gradient at 4 mL/min. Recoveries of benzyl, 4-hydroxybenzyl, and allylglucosinolates added to plant extracts were quantitative.
1977 ◽
Vol 60
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pp. 935-939
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1984 ◽
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pp. 1135-1137
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1979 ◽
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pp. 171-175
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1977 ◽
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pp. 1307-1309
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1986 ◽
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pp. 856-859
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1995 ◽
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pp. 841-845
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