scholarly journals Determination of Olaquindox Residues in Swine Tissues by Liquid Chromatography

1987 ◽  
Vol 70 (4) ◽  
pp. 706-707 ◽  
Author(s):  
Tomoko Nagata ◽  
Masanobu Saeki
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Column Chromatography ◽  
Alumina Column ◽  
Ultraviolet Detection ◽  
Alumina Column Chromatography ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for determination of olaquindox residues in swine tissues. The drug is extracted from tissues with acetonitrile, and the extract is evaporated to dryness. This residue is cleaned up by alumina column chromatography. LC analysis is carried out on a Nucleosil C18 column, and olaquindox is quantitated by ultraviolet detection at 350 nm. The average recoveries of olaquindox added to tissues at levels of 0.2, 0.1, and 0.05 ppm were 74.0, 68.6, and 66.3%, respectively. The detection limit was 2 ng for olaquindox standard and 0.02 ppm in tissues.

Liquid Chromatographic Determination of the Herbicide Isoxaben and Its Soil Metabolite in Soil and Soil-Turf Samples

1990 ◽  
Vol 73 (2) ◽  
pp. 287-289 ◽  
Author(s):  
Bonnie S Rutherford
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Column Chromatography ◽  
Chromatographic Determination ◽  
Alumina Column ◽  
Alumina Column Chromatography ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method Is described for the determination of residues of isoxaben and its principal soil metabolite In soil and soil-turf samples. Both compounds are extracted from samples by refluxing with methanol-water. An aliquot of the extract Is partitioned Into dichloromethane and purified by alumina column chromatography. Separate fractions containing isoxaben and metabolite are collected and subjected to liquid chromatography at conditions that are optimized for each compound. The detection limit for both compounds is 0.005 ppm. Residue identities are confirmed by chromatography on a different LC system.

Liquid Chromatographic Determination of Sulfamoyldapsone in Swine Tissues

1987 ◽  
Vol 70 (6) ◽  
pp. 1031-1032
Author(s):  
Yuuko S Endoh ◽  
Ryozo Yamaoka ◽  
Nobuo Sasaki
Keyword(s):  
Detection Limit ◽  
Quantitative Determination ◽  
Chromatographic Determination ◽  
Alumina Column ◽  
Average Recovery ◽  
Diaminodiphenyl Sulfone ◽  
Alumina Column Chromatography ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for the quantitative determination of sulfamoyldapsone (2-sulfamoyl-4,4'-diaminodiphenyl sulfone) in swine muscle, liver, kidney, and fat. Sulfamoyldapsone was extracted from tissues with acetonitrile saturated with n-hexane. The extract was washed with n-hexane saturated with acetonitrile, concentrated, and cleaned up by alumina column chromatography. Sulfamoyldapsone was separated on an ODS column by using acetonitrile-methanol-water (6 + 18 + 76) and was detected at 292 nm. Overall average recovery of sulfamoyldapsone added to tissues at levels of 0.1 and 0.5 /μg/g was 93.3% ± 6.0. Detection limit was 0.02 μg/g in these tissues.

Determination of Ampicillin Residues in Fish Tissues by Liquid Chromatography

1986 ◽  
Vol 69 (3) ◽  
pp. 448-450 ◽  
Author(s):  
Tomoko Nagata ◽  
Masanobu Saeki
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Fish Tissues ◽  
Ultraviolet Detection ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for determination of ampicillin residues in fish tissues. The drug is extracted from tissues with methanol, and the extract is evaporated to dryness. This residue is cleaned up by Florisil cartridge chromatography. LC analysis is carried out on a Nucleosil C18 column, and ampicillin is quantitated by ultraviolet detection at 222 nm. Recoveries of ampicillin added to tissues at levels of 0.2 and 0.1 ppm were 73.2 and 61.5%, respectively. The detection limit was 3 ng for ampicillin standard, and 0.03 ppm in tissues.

Rapid Determination of Deoxynivalenol (Vomitoxin) by Liquid Chromatography Using Modified Romer Column Cleanup

1984 ◽  
Vol 67 (1) ◽  
pp. 52-54 ◽  
Author(s):  
Henry L Chang ◽  
Jonathan W Devries ◽  
Paul A Larson ◽  
Hasmukh H Patel
Keyword(s):  
Liquid Chromatography ◽  
Standard Deviation ◽  
Mobile Phase ◽  
Rapid Determination ◽  
Alumina Column ◽  
Relative Standard ◽  
Alumina Column Chromatography ◽  
Wheat Products ◽  
Liquid Chromatographic

Abstract A modification of the Romer method for determining deoxynivalenol (DON) provides rapid sample cleanup and includes liquid chromatographic (LC) quantitation. The method was evaluated using wheat, wheat flour, and other wheat products. The sample is extracted with acetonitrile–water (84 + 16), and an aliquot of the extract is subjected to activated charcoal–alumina column chromatography. The extract is then evaporated and diluted to volume with mobile phase, and DON is quantitated using liquid chromatography. The relative standard deviation based on duplicate samples is 6.07%. The detection limit is 30 ppb based on 2 x signal/noise ratio. Results by this method compared with the results obtained by the Scott GC method showed a correlation coefficient of 0.992 with a mean vomitoxin content of 779 ppb by this method and 716 ppb by the Scott method for 14 samples.

Determination of Melengestrol Acetate in Feedstuffs with Liquid Chromatographic Preparatory Column Cleanup and Quantitative Analysis

1988 ◽  
Vol 71 (4) ◽  
pp. 707-709
Author(s):  
Jim L Weigand ◽  
Daniel S Dille
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Sodium Sulfate ◽  
Alumina Column ◽  
Melengestrol Acetate ◽  
Preparative Lc ◽  
Time Savings ◽  
Commercial Feeds ◽  
Liquid Chromatographic

Abstract Melengestrol acetate (MGA) is determined by liquid chromatography using a fraction from preparatory LC as a means of sample cleanup for feedstuffs, both dry and liquid. Dry ground feed is Soxhlet extracted with hexane and passed through a 2% deactivated alumina column for initial cleanup. The eluate is evaporated, redissolved in methanol, filtered, and injected onto a preparatory LC column. The fraction containing MGA is separated from the remaining matrix, evaporated to dryness, dissolved in methanol, and quantitated by LC analysis. Liquid supplements are extracted in methanol, and the extract is evaporated to near dryness. The residue is diluted with water, extracted with chloroform, passed through sodium sulfate, and evaporated to dryness. The remaining sample is dissolved in methanol prior to preparative LC and quantitative LC. Recoveries for 2 laboratory- fortified commercial feeds, one dry and one liquid, containing 0.39 and 0.40 mg/lb, were 98.3% ± 4.4 and 95.8% ± 4.3, respectively. Results compare favorably with existing methods. Up to a 4-fold time savings was realized by this method without automation.

Liquid Chromatographic Determination of Fluridone Aquatic Herbicide and Its Metabolite in Fish and Crayfish

1986 ◽  
Vol 69 (5) ◽  
pp. 856-859 ◽  
Author(s):  
Sheldon D West ◽  
Edgar W Day
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
Reverse Phase Liquid Chromatography ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Aquatic Herbicide ◽  
Liquid Chromatographic

Abstract A residue method is described for determination of the aquatic herbicide fluridone (1-methy1-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)- pyridinone) and its metabolite (1-methy1-3-(4-hydroxyphenyl)-5-[3- (trifluoromethyl)phenyl]-4(1H)-pyridinone) in fish and crayfish tissues. Both compounds are extracted from tissues with methanol, and the extracts are subjected to acidic hydrolysis to release conjugated forms of fluridone and the metabolite. Sample extracts are purified by liquidliquid partitioning and Florisil Sep-Pak® column chromatography. Both compounds are separated and measured by reverse phase liquid chromatography with UV detection at 313 nm. In the absence of interfering peaks, the method has a detection limit of approximately 0.04 ppm of either compound. Overall, recoveries averaged 96% for fluridone and 78% for the metabolite for all tissue types combined.

Liquid Chromatographic Determination of Bromide Ion in Cereals, Fruit, Vegetables, and Blood with a Silver Electrode in an Electrochemical Detector System

1995 ◽  
Vol 78 (3) ◽  
pp. 841-845 ◽  
Author(s):  
Bertil Lindgren ◽  
Tomas Berglöf ◽  
Åsa Ramberg ◽  
Anna Stepdmska ◽  
Malin Åkerblom
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Rapid Determination ◽  
Chromatographic Determination ◽  
Electrochemical Detector ◽  
Satisfactory Precision ◽  
Bromide Ion ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method is presented for rapid determination of bromide ion in commodities and blood by paired-ion liquid chromatography with electrochemical detection. The method involves extraction of samples with water and filtration. Blood is passed through a Sep-Pak C18 minicolumn. Recoveries are usually close to 100%, with satisfactory precision. The detection limit is 1 mg/kg. The method needs little labor and uses no noxious solvents or reagents.

Simultaneous Liquid Chromatographic Determination of Thiabendazole, o-Phenylphenol, and Diphenyl Residues in Citrus Fruits, Without Prior Cleanup

1982 ◽  
Vol 65 (6) ◽  
pp. 1302-1304
Author(s):  
Yoshimi Kitada ◽  
Michiko Sasaki ◽  
Kaoru Tanigawa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Citrus Fruits ◽  
Ultraviolet Detection ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple, rapid, efficient method has been developed for determining thiabendazole, o-phenylphenol, and diphenyl in citrus fruits by using high performance liquid chromatography, with fluorescence or ultraviolet detection. The compounds are extracted with ethyl acetate and separated from soluble fruit components on a LiChrosorb RP-8 column. Recovery of these compounds added to citrus fruits at 5 or 50 ppm levels was >93%; the limit of detection for the compounds is 1 ppm.

Gas-Liquid Chromatographic Determination of Chlorinated Benzenes and Phenols in Selected Biological Matrices

1980 ◽  
Vol 63 (1) ◽  
pp. 27-32
Author(s):  
Lester L Lamparski ◽  
Marsha L Langhorst ◽  
Terry J Nestrick ◽  
Sergio Cutié
Keyword(s):  
Column Chromatography ◽  
Chromatographic Determination ◽  
Chlorinated Phenols ◽  
Acid Digestion ◽  
Chlorinated Benzenes ◽  
Fish Flesh ◽  
Alumina Column Chromatography ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract The determination of higher chlorinated benzenes and chlorinated phenols at parts per billion or parts per million levels in biological samples is described. The method includes sulfuric acid digestion, silica gel column chromatography, methylation, alumina column chromatography, and detection by electron capture gas chromatography. Recoveries are reported (or fish flesh, rabbit tissues, and duck tissues with detection limits of 10-15 ppb (ng/g tissue).

High-Speed Liquid Chromatographic Cleanup of Environmental Samples Prior to the Gas Chromatographic Determination of Lindane

1974 ◽  
Vol 57 (5) ◽  
pp. 1046-1049
Author(s):  
Richard H Larose
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Environmental Samples ◽  
High Speed ◽  
Chromatographic Determination ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatograph ◽  
Cleanup Procedure ◽  
Liquid Chromatographic

Abstract A method is described which permits the removal of interfering co-extractives from lindane, using high-speed liquid chromatography. Fractions are collected from the liquid chromatograph for further analysis by gas-liquid chromatography. The cleanup procedure takes less than 5 min and recoveries of more than 90% are obtained. The detection limit for water samples is 5 ng/L.

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