scholarly journals Simultaneous Identification and Quantitative Determination of Selected Aminoglycoside Antibiotics by Thin-Layer Chromatography and Densitometry

2009 ◽  
Vol 92 (4) ◽  
pp. 1068-1075 ◽  
Author(s):  
Urszula Hubicka ◽  
Jan Krzek ◽  
Hanna Woltyska ◽  
Boóena Stachacz
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
High Sensitivity ◽  
Good Separation ◽  
Fluorescent Indicator ◽  
Tlc Plates ◽  
Simultaneous Identification ◽  
Layer Chromatography

Abstract A TLCdensitometric method has been developed for simultaneous identification and quantitative determination of amikacin, gentamicin, kanamycin, neomycin, netilmicin, and tobramycin. This separation of antibiotics was achieved on silica gel TLC plates without a fluorescent indicator and with methanol25 ammoniachloroform (3 + 2 + 1, v/v/v) as the mobile phase. The densitometric measurements were made at 500 nm after detection with a 0.2 ninhydrin solution in ethanol. Under these conditions, good separation of the chosen aminoglycosides was obtained. The method is distinguished by high sensitivity, with the LOD from 0.25 g for amikacin to 1.00 g for gentamicin and the LOQ from 0.5 g for amikacin to 1.65 g for gentamicin, and a wide linearity range 0.756.25 g/spot for amikacin and netilmicin and 1.512.50 g/spot for other antibiotics. The precision of the determination was very good; RSD varied in the range 0.30.6.

scholarly journals Determination of New Antidepressants in Pharmaceuticals by Thin-Layer Chromatography with Densitometry

2010 ◽  
Vol 93 (3) ◽  
pp. 778-782 ◽  
Author(s):  
Tatána Gondová ◽  
Iveta Petríková
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Simultaneous Separation ◽  
Tlc Plates ◽  
Precision And Accuracy ◽  
Layer Chromatography

Abstract A new and simple TLC-densitometry method has been developed for the simultaneous separation of the two noradrenergic and specific serotonergic antidepressants mirtazapine and mianserine and validated for their determination in commercially available tablets. The method used TLC plates precoated with silica gel 60F254 as the stationary phase, and the mobile phase consisted of hexaneisopropanol25 ammonia (70 + 25 + 5, v/v/v). Densitometric analysis was carried out in the absorbance mode at 280 nm. The method was validated in accordance with International Conference on Harmonization guidelines in terms of linearity, LOD, LOQ, precision, and accuracy. Calibration curves were linear (R2 > 0.9970) with respect to peak area in the concentration range of 5002500 and 5005000 ng/spot for mirtazapine and mianserine, respectively. The LODs were 20 and 35 ng/spot for mirtazapine and mianserine, respectively. The described method was successfully applied to the determination of mirtazapine and mianserine in their pharmaceutical formulations with recovery ranging from 99.83 to 101.20 of the labeled amount of the compounds. The proposed method can be used in routine QC of these drugs in pharmaceutical formulations.

IDENTIFIKASI DAN PENETAPAN KADAR RHODAMIN B DALAM KERUPUK BERWARNA MERAH YANG BEREDAR DI PASAR ANTASARI KOTA BANJARMASIN

2017 ◽  
Vol 1 (1) ◽  
pp. 85
Author(s):  
Eka Kumalasari
Keyword(s):  
Thin Layer ◽  
Rhodamine B ◽  
Textile Industry ◽  
Mobile Phase ◽  
Uv Lamp ◽  
Tapioca Flour ◽  
Red Dye ◽  
Layer Chromatography ◽  
Rhodamin B

Crackers are made from tapioca flour batter mixed with flavorings and colorings, still many outstanding crackers that contain ingredients banned dye Rhodamine B. Rhodamine B is a chemical used for red dye in the textile industry and plastic. Rhodamine B can cause cancer, poisoning, lung irritation, sore eyes, and sore throat. This study aims to identify and determination the levels of Rhodamine B in circulating red crackers Antasari market Banjarmasin.The population is that sold in the red crackers that sold in Antasari market Banjarmasin.. The sampling is technique incidental sampling , that is based on chance, so any population by chance met with researchers can be used as a sample. Identification of Rhodamine B was done by Thin Layer Chromatography (TLC) by using the stationary phase silica GF 254 and mobile phase is elution solvent is n-butanol, ethyl acetate, ammonia (10:4:5). Then detected with a UV lamp 254 nm and 366 nm. While for the determination of levels using Vis spectrophotometry at a wavelength of 544 nm.The results showed that the samples of 6 found one sample containing Rhodamine B, namely samples 5 (cassava crackers matches) and obtained values of 7,25 ± 3,8640 levels mg / kg. Based on these results, Rhodamine B still found in crackers that sold in the market Antasari Banjarmasin.

scholarly journals Chiral Separation of Diastereoisomers of Cefuroxime Axetil by High-Performance Thin-Layer Chromatography

2010 ◽  
Vol 93 (3) ◽  
pp. 771-777 ◽  
Author(s):  
Monika Dbrowska ◽  
Jan Krzek
Keyword(s):  
High Performance ◽  
High Sensitivity ◽  
Cefuroxime Axetil ◽  
Spectrometric Analysis ◽  
Good Separation ◽  
High Recovery ◽  
Cellulose Layers ◽  
The Individual ◽  
Layer Chromatography

Abstract Conditions for separation and determination of diastereoisomers of cefuroxime axetil by HPTLC on cellulose layers with densitometry were developed. A good separation of the constituents was achieved using a mobile phase composed of 1 aqueous -cyclodextrinmethanol (15 + 1, v/v). For detecting spots on the chromatograms, densitometric measurements were used at 285 nm. The method is characterized by high sensitivity; the LOD was 0.04 g/spot and LOQ was 0.11 g/spot for both forms of the cefuroxime axetil diastereoisomers. For the individual diastereoisomers, high recovery values from 96.63 to 104.16 were obtained. Identification of the diastereoisomers was performed by 1H-NMR spectrometric analysis.

Notizen: Improved 2,4-Dinitrophenylhydrazine, Thin Layer Chromatography Methods for the Determination of Micro- and Macroamounts of Ascorbic Acid

1974 ◽  
Vol 29 (11-12) ◽  
pp. 777-780 ◽  
Author(s):  
A. Navon ◽  
H. Z. Levinson
Keyword(s):  
Acetic Acid ◽  
Vitamin C ◽  
Thin Layer ◽  
Condensation Reaction ◽  
Dehydroascorbic Acid ◽  
Previous Method ◽  
Aqueous Acetic Acid ◽  
Tlc Plates ◽  
Layer Chromatography

Microamounts of vitamin C could be readily determined in 20 μl-samples using the 2,4-dinitrophenylhydrazine method together with separation by thin layer chromato­graphy. The condensation reaction was carried out for 5 min at 100 °C on a glass fibre disc. Purification of vitamin C hydrazones was accomplished by repeated separation on TLC plates. An aqueous solution of 65% acetic acid was em­ployed to dissolve the vitamin C hydrazones, providing maxi­mal absorbance at 500 nm. The minimum amount detectable by this method is 0.4 μg of dehydroascorbic acid. The macrodetermination of vitamin C was improved by simpli­fying a previous method and employing 65% aqueous acetic acid as a solvent for the hydrazones.

TLC Determination of Aquatic Humic Acids

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2567-2570 ◽  
Author(s):  
M. Kaštelan-Macan ◽  
Š. Cerjan-Stefanovic ◽  
D. Jalšovec
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Humic Acids ◽  
Visible Spectrum ◽  
Solvent System ◽  
Spectroscopic Methods ◽  
Precise Method ◽  
Adsorption Resin ◽  
Layer Chromatography

Humic acids are mostly determined by spectroscopic methods, although they are not accurate enough, because humic acids possess no maximum absorption at any wavelength in the ultraviolet and visible spectrum. In this contribution the accurate and precise method for aquatic humic acids determination, using thin layer chromatography, was worked out. Previously, humic acids were separated and preconcentrated -from the river water, using macroreticular adsorption resin Amberlite XAD-4, and subsequently determined by TLC. The Chromatographie system was: silicagel HF254(Merck, precoated plates 20 × 20 cm) and NH3 -acetone (60:40,v/v) as a solvent system. Quantitative determination was made by Camag– Turner 111 Fluorimeter, by measuring UV–quenching.

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