261 Effects of Dam Nutrition on Offspring Metabolism

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 138-138
Author(s):  
Kristen E Govoni
Keyword(s):  
Metabolic Regulation ◽  
Maternal Nutrition ◽  
Maternal Diet ◽  
Local Level ◽  
Tissue Level ◽  
Leptin Resistance ◽  
Metabolic Profiles ◽  
Sheep Model ◽  
Metabolic Dysregulation ◽  
Altered Metabolism

Abstract Maternal diet during gestation is important for proper fetal growth and development. There is evidence that poor maternal nutrition (restricted- and over-feeding) can alter growth of the fetus with long-term consequences on postnatal growth and adult maintenance. Additionally, maternal diet can program offspring for altered metabolism which leads to increased fat deposition and likely reduced efficiency of production later in life. Using livestock models, we and others have demonstrated insulin resistance, leptin resistance, and increased adiposity in response to poor maternal nutrition. These systemic changes are likely due to altered metabolic regulation at the tissue level. Using a sheep model, we have evidence that poor maternal nutrition also alters key metabolic profiles in muscle and liver, key metabolic tissues, in offspring. Specifically, in offspring of restricted-fed ewes, similar profiles of amino acid (e.g. branched-chain amino acids, histidine, methionine) and lipid (e.g. triglycerides) metabolites were altered in blood, liver, and muscle. In the muscle of offspring from restricted- and over-fed ewes, lipid and protein metabolic profiles diverged between the two treatment groups. This demonstrates different mechanisms contributing to altered metabolism of offspring from ewes restricted- and over-fed during gestation. The similar changes in metabolic profiles at both the systemic and local level suggest complex mechanisms involved in metabolic dysregulation of offspring from poorly fed mothers, which likely contribute to life-long metabolic dysregulation and reduced efficiency of growth and product quality.

Lifestyle, Maternal Nutrition and Healthy Pregnancy

2020 ◽  
Vol 19 (2) ◽  
pp. 132-140
Author(s):  
Alfonso Mate ◽  
Claudia Reyes-Goya ◽  
Álvaro Santana-Garrido ◽  
Carmen M. Vázquez
Keyword(s):  
Physical Activity ◽  
Maternal Nutrition ◽  
Muscle Tone ◽  
Maternal Diet ◽  
Perinatal Outcomes ◽  
Psychosocial Health ◽  
World Health ◽  
Large For Gestational Age ◽  
Moderate Physical Activity ◽  
Healthy Pregnancy

: Healthy lifestyle habits spanning from preconception to postpartum are considered as a major safeguard for achieving successful pregnancies and for the prevention of gestational diseases. Among preconception priorities established by the World Health Organization (WHO) are healthy diet and nutrition, weight management, physical activity, planned pregnancy and physical, mental and psychosocial health. Most studies covering the topic of healthy pregnancies focus on maternal diet because obesity increases the risks for adverse perinatal outcomes, including gestational diabetes mellitus, large for gestational age newborns, or preeclampsia. Thus, foods rich in vegetables, essential and polyunsaturated fats and fibre-rich carbohydrates should be promoted especially in overweight, obese or diabetic women. An adequate intake of micronutrients (e.g. iron, calcium, folate, vitamin D and carotenoids) is also crucial to support pregnancy and breastfeeding. Moderate physical activity throughout pregnancy improves muscle tone and function, besides decreasing the risk of preeclampsia, gestational diabesity (i.e. diabetes associated with obesity) and postpartum overweight. Intervention studies claim that an average of 30 min of exercise/day contributes to long-term benefits for maternal overall health and wellbeing. Other factors such as microbiome modulation, behavioural strategies (e.g. smoking cessation, anxiety/stress reduction and sleep quality), maternal genetics and age, social class and education might also influence the maternal quality of life. These factors contribute to ensure a healthy pregnancy, or at least to reduce the risk of adverse maternal and foetal outcomes during pregnancy and later in life.

Differential Effects of Maternal Diets on Birth Outcomes and Metabolic Parameters in Rats After Ethanol Consumption during Pregnancy

2020 ◽  
pp. 1-25
Author(s):  
Yidi Wang ◽  
Bradley A. Feltham ◽  
Michael N. A. Eskin ◽  
Miyoung Suh
Keyword(s):  
Total Cholesterol ◽  
Birth Outcomes ◽  
Maternal Nutrition ◽  
Maternal Diet ◽  
Hdl Cholesterol ◽  
Nutrition Intervention ◽  
Metabolic Parameters ◽  
Nutrition Status ◽  
Sprague Dawley ◽  
Metabolic Outcomes

Abstract Maternal nutrition status plays an important role in the development of fetal alcohol spectrum disorders (FASD), but its direct evidence is lacking. This study compared a standard chow with a semi-purified energy dense (E-dense) diet on birth and metabolic outcomes in rats after ethanol (EtOH) consumption during pregnancy. Pregnant Sprague-Dawley rats were randomized into four groups: chow (n=6), chow+EtOH (20% v/v) (n=7), E-dense (n=6), and E-dense+EtOH (n=8). Birth outcomes including litter size, body and organ weights were collected. Metabolic parameters were measured in dams and pups at postnatal day (PD) 7. Maternal EtOH consumption decreased body weights (p <0.0001) and litter sizes (p <0.05) in chow-fed dams. At PD7, pups born to dams fed E-dense diet had higher body (p <0.002) and liver weights (p <0.0001). These pups also had higher plasma total cholesterol (p <0.0001), triacyclglycerol (p <0.003) and alanine aminotransferase (p <0.03) compared to those from chow-fed dams. Dams fed E-dense diet had higher plasma total- (p <0.0001) and HDL-cholesterol (p <0.0001) and lower glucose (p <0.0001). EtOH increased total cholesterol (p <0.03) and glucose (p <0.05) only in dams fed the E-dense diet. Maternal exposure to E-dense diet attenuated prenatal EtOH-induced weight loss and produced different metabolic outcomes in both dams and pups. While the long-lasting effects of these outcomes are unknown, this study highlights the importance of maternal diet quality for maternal health and infant growth, and suggests that maternal nutrition intervention may be a potential target for alleviating FASD.

scholarly journals Activation of mitochondrial TUFM ameliorates metabolic dysregulation through coordinating autophagy induction

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Dasol Kim ◽  
Hui-Yun Hwang ◽  
Eun Sun Ji ◽  
Jin Young Kim ◽  
Jong Shin Yoo ◽  
...  
Keyword(s):  
Metabolic Regulation ◽  
Elongation Factor ◽  
Dependent Manner ◽  
Diet Induced Obesity ◽  
Metabolic Dysregulation ◽  
Autophagy Induction ◽  
Transcription Factor Eb ◽  
Mitochondrial Elongation

AbstractDisorders of autophagy, a key regulator of cellular homeostasis, cause a number of human diseases. Due to the role of autophagy in metabolic dysregulation, there is a need to identify autophagy regulators as therapeutic targets. To address this need, we conducted an autophagy phenotype-based screen and identified the natural compound kaempferide (Kaem) as an autophagy enhancer. Kaem promoted autophagy through translocation of transcription factor EB (TFEB) without MTOR perturbation, suggesting it is safe for administration. Moreover, Kaem accelerated lipid droplet degradation in a lysosomal activity-dependent manner in vitro and ameliorated metabolic dysregulation in a diet-induced obesity mouse model. To elucidate the mechanism underlying Kaem’s biological activity, the target protein was identified via combined drug affinity responsive target stability and LC–MS/MS analyses. Kaem directly interacted with the mitochondrial elongation factor TUFM, and TUFM absence reversed Kaem-induced autophagy and lipid degradation. Kaem also induced mitochondrial reactive oxygen species (mtROS) to sequentially promote lysosomal Ca2+ efflux, TFEB translocation and autophagy induction, suggesting a role of TUFM in mtROS regulation. Collectively, these results demonstrate that Kaem is a potential therapeutic candidate/chemical tool for treating metabolic dysregulation and reveal a role for TUFM in autophagy for metabolic regulation with lipid overload.

106 The Effects of Fetal Programming on Offspring Pancreas DNA Methylation

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 54-55
Author(s):  
Maria L Hoffman
Keyword(s):  
Dna Methylation ◽  
Fetal Programming ◽  
Maternal Nutrition ◽  
Maternal Diet ◽  
Multiple Generations ◽  
Livestock Species ◽  
Maternal Programming ◽  
Genome Bisulfite Sequencing ◽  
The Many ◽  
Methylation Patterns

Abstract It has been well documented that fetal programming, caused by changes to the maternal environment during pregnancy, can impact the overall health and growth of the offspring in livestock and non-livestock species alike. These effects are observed in the F1 offspring as well as across subsequent generations; however, the mechanisms by which this occurs are still poorly understood. Epigenetics is one of the many mechanisms that is hypothesized to have a role in fetal programming and may be mediating the observed effects across multiple generations. It has been demonstrated by others that DNA methylation patterns can be altered by an individuals’ diet and that the pancreas is vulnerable to the effects of fetal programming. Therefore, we evaluated the effects of poor maternal nutrition during gestation on the pancreas tissue of lambs. We have demonstrated that maternal under- or overnutrition during gestation alters the DNA methylation patterns of the offspring pancreas tissue with these effects being diet dependent and sex specific. We have also begun evaluating the effects of maternal diet in murine models using whole-genome bisulfite sequencing to compare species differences and determine if there are any changes conserved across species. This will allow us to focus on a smaller number of critical factors in individuals as they age and across multiple generations in livestock species such as sheep and cattle. From these data we will be able to elucidate the role DNA methylation has in mediating the effects of maternal programming in the pancreas tissue.

scholarly journals Sexual dimorphism in hypothalamic inflammation in the offspring of dams exposed to a diet rich in high fat and branched-chain amino acids

2019 ◽  
Vol 317 (3) ◽  
pp. E526-E534 ◽  
Author(s):  
Marianna Sadagurski ◽  
Lucas Kniess Debarba ◽  
Joao Pedro Werneck-de-Castro ◽  
Abear Ali Awada ◽  
Tess A. Baker ◽  
...  
Keyword(s):  
Energy Homeostasis ◽  
Maternal Nutrition ◽  
Inflammatory Responses ◽  
Maternal Diet ◽  
Female Offspring ◽  
Metabolic Effects ◽  
Standard Diet ◽  
High Fat ◽  
Hypothalamic Inflammation ◽  
Branched Chain

Branched-chain amino acid (BCAAs: leucine, isoleucine, and valine) contribute to the development of obesity-associated insulin resistance in the context of consumption of a high-fat diet (HFD) in humans and rodents. Maternal diet is a major determinant of offspring health, and there is strong evidence that maternal HFD alters hypothalamic developmental programming and disrupts offspring energy homeostasis in rodents. In this study, we exposed pregnant and lactating C57BL/6JB female mice to either HFD, HFD with supplemented BCAA (HFD+BCAA), or standard diet (SC), and we studied offspring metabolic phenotypes. Both maternal HFD and HFD supplemented with BCAA had similar effect rendering the offspring metabolic imbalance and impairing their ability to cope with HFD when challenged during aging. The metabolic effects of HFD challenge were more profound in females, worsening female offspring ability to cope with an HFD challenge by activating hypothalamic inflammation in aging. Moreover, the sex differences in hypothalamic estrogen receptor α (ER-α) expression levels were lost in female offspring upon HFD challenge, supporting a link between ER-α levels and hypothalamic inflammation in offspring and highlighting the programming potential of hypothalamic inflammatory responses and maternal nutrition.

scholarly journals Analysis of microscopic bone properties in an osteoporotic sheep model: a combined biomechanics, FE and ToF-SIMS study

2019 ◽  
Vol 16 (151) ◽  
pp. 20180793 ◽  
Author(s):  
R. Müller ◽  
A. Henss ◽  
M. Kampschulte ◽  
M. Rohnke ◽  
A. C. Langheinrich ◽  
...  
Keyword(s):  
Biomechanical Testing ◽  
Tissue Level ◽  
Osteoporotic Bone ◽  
Compression Tests ◽  
Micro Computed Tomography ◽  
Deficient Diet ◽  
Element Analysis ◽  
Sheep Model ◽  
Tissue Properties ◽  
Bone Properties

The present study deals with the characterization of bone quality in a sheep model of postmenopausal osteoporosis. Sheep were sham operated ( n = 7), ovariectomized ( n = 6), ovariectomized and treated with deficient diet ( n = 8) or ovariectomized, treated with deficient diet and glucocorticoid injections ( n = 7). The focus of the study is on the microscopic properties at tissue level. Microscopic mechanical properties of osteoporotic bone were evaluated by a combination of biomechanical testing and mathematical modelling. Sample stiffness and strength were determined by compression tests and finite-element analysis of stress states was conducted. From this, an averaged microscopic Young’s modulus at tissue level was determined. Trabecular structure as well as mineral and collagen distribution in samples of sheep vertebrae were analysed by micro-computed tomography and time-of-flight secondary ion mass spectrometry. In the osteoporotic sheep model, a disturbed fibril structure in the triple treated group was observed, but bone loss only occurred in form of reduced trabecular number and thickness and cortical decline, while quality of the residual bone was preserved. The preserved bone tissue properties in the osteoporotic sheep model allowed for an estimation of bone strength which behaves similar to the human case.

Relationship of maternal high-fat diet during pregnancy and lactation to offspring health

2020 ◽  
Author(s):  
Kinga Gawlińska ◽  
Dawid Gawliński ◽  
Małgorzata Filip ◽  
Edmund Przegaliński
Keyword(s):  
High Fat Diet ◽  
Maternal Nutrition ◽  
System Development ◽  
Maternal Diet ◽  
Nervous System Development ◽  
High Fat ◽  
Preclinical Research ◽  
Intrauterine Development ◽  
Increased Risk ◽  
Pregnancy And Lactation

Abstract A balanced maternal diet is essential for proper fetal development, and the consumption of a nutritionally inadequate diet during intrauterine development and early childhood is associated with a significantly increased risk of metabolic and brain disorders in offspring. The current literature indicates that maternal exposure to a high-fat diet exerts an irreversible influence on the general health of the offspring. This review of preclinical research examines the relationship between a maternal high-fat diet during pregnancy or lactation and metabolic changes, molecular alterations in the brain, and behavioral disorders in offspring. Animal models indicate that offspring exposed to a maternal high-fat diet during pregnancy and lactation manifest increased depressive-like and aggressive behaviors, reduced cognitive development, and symptoms of metabolic syndrome. Recently, epigenetic and molecular studies have shown that maternal nutrition during pregnancy and the suckling period modifies the development of neurotransmitter circuits and many other factors important to central nervous system development. This finding confirms the importance of a balanced maternal diet for the health of offspring.

Effects of selection for production and maternal diet on maiden dairy heifer fertility

2002 ◽  
Vol 74 (3) ◽  
pp. 415-421 ◽  
Author(s):  
J. E. Pryce ◽  
G. Simm ◽  
J. J. Robinson
Keyword(s):  
Reproductive Performance ◽  
Maternal Nutrition ◽  
Maternal Diet ◽  
Limited Range ◽  
Research Centre ◽  
Selection Line ◽  
Control Line ◽  
Maternal Environment ◽  
Significant Relationships ◽  
The Uk

AbstractThe objectives of this study were to investigate reproductive performance and the influence of maternal environment on reproductive performance in two genetic lines of maiden heifers. These were given food and managed in the same way at the Langhill Dairy Cattle Research Centre. The two genetic lines were established in 1973; one has been selected for high combined yield of fat plus protein (selection line; S) and the other has been maintained at around the UK average for genetic merit of yield of fat plus protein (control line; C). Analysis of the reproductive performance of 988 heifers born between 1981 and 1998 showed that S heifers were poorer (all P < 0·05) than C heifers for all reproductive measures: conception at first service (S: 0·64, s.e. 0·02; C: 0·71, s.e. 0·03), interval between first and last service (S: 18·2 days, s.e. 2·2; C: 13·4 days, s.e. 2·2) and number of services per conception (S: 1·49 services, s.e. 0·06; C: 1·39 services, s.e. 0·06). S heifers were also younger at first service than C heifers (474·9 days v. 480·1 days, s.e. 1·9, for S and C respectively; P • 01). Fertility of service sires may have had some influence on these results, but this could not be investigated here, as S heifers were mated only to high merit bulls and C heifers to average merit bulls. However, within genetic line, the yearly downward trend in the average number of services per conception of heifers was significantly different from zero for the S line, but not the C line. There were no statistically significant relationships between conception rates in maiden heifers and their subsequent reproductive performance in first lactation. The effect of maternal environment on the reproductive performance of daughters as maiden heifers was investigated. There were no statistically significant relationships between daughter reproductive performance and dam parity or the feeding system of the dam (either a high or low level of concentrates). Within the limited range of nutritional status of dams during the periods post calving and in early pregnancy, there was no statistically significant effect of maternal nutrition on daughter reproductive performance.

scholarly journals Fetal and organ development at gestational days 45, 90, 135 and at birth of lambs exposed to under- or over-nutrition during gestation1,2,3

2017 ◽  
Vol 1 (1) ◽  
pp. 16-25 ◽  
Author(s):  
S. M. Pillai ◽  
A. K. Jones ◽  
M. L. Hoffman ◽  
K. K. McFadden ◽  
S. A. Reed ◽  
...  
Keyword(s):  
Maternal Nutrition ◽  
Maternal Diet ◽  
The Body ◽  
Heart Weight ◽  
Crown Rump Length ◽  
Organ Growth ◽  
Gland Weight ◽  
Randomized Design ◽  
Kidney Length ◽  
Perirenal Fat

Abstract To determine the effects of poor maternal nutrition on offspring body and organ growth during gestation, pregnant Western White-faced ewes (n = 82) were randomly assigned into a 3 × 4 factorial treatment structure at d 30.2 ± 0.2 of gestation (n = 5 to 7 ewes per treatment). Ewes were individually fed 100% (control), 60% (restricted) or 140% (over) of NRC requirements for TDN. Ewes were euthanized at d 45, 90 or 135 of gestation or underwent parturition (birth) and tissues were collected from the offspring (n = 10 to 15 offspring per treatment). Offspring from control, restricted and overfed ewes are referred to as CON, RES and OVER, respectively. Ewe data were analyzed as a completely randomized design and offspring data were analyzed as a split-plot design using PROC MIXED. Ewe BW did not differ at d 30 (P ≥ 0.43), however restricted ewes weighed less than overfed and overfed were heavier than controls at d 45, and restricted weighed less and overfed were heavier than controls at d 90 and 135 and birth (P ≤ 0.05). Ewe BCS was similar at d 30, 45 and 90 (P ≤ 0.07), however restricted ewes scored lower than control at d 135 and birth (P ≤ 0.05) and over ewes scored higher than control at d 135 (P ≤ 0.05) but not at birth (P = 0.06). A maternal diet by day of gestation interaction indicated that at birth the body weight (BW) of RES offspring was less than CON and OVER (P ≤ 0.04) and heart girth of RES was smaller than CON and OVER (P ≤ 0.004). There was no interaction of maternal diet and day of gestation on crown-rump, fetal, or nose occipital length, or orbit or umbilical diam. (P ≥ 0.31). A main effect of maternal diet indicated that the RES crown-rump length was shorter than CON and OVER (P ≤ 0.05). An interaction was observed for liver, kidney and renal fat (P ≤ 0.02). At d 45 the liver of RES offspring was larger than CON and OVER (P ≤ 0.002), but no differences observed at d 90, 135 or birth (P ≥ 0.07). At d 45, the kidneys of OVER offspring were larger than CON and RES (P ≤ 0.04), but no differences observed at d 90, 135 or birth (P ≥ 0.60). At d 135, OVER had more perirenal fat than CON and RES (P ≤ 0.03), and at birth RES had more perirenal fat than CON and OVER (P ≤ 0.04). There was no interaction observed for offspring heart weight, length or width, kidney length, adrenal gland weight, loin eye area or rib width (P ≥ 0.09). In conclusion, poor maternal nutrition differentially alters offspring body size and organ growth depending on the stage of gestation.

scholarly journals Long-term consequences of maternal high-fat feeding on hypothalamic leptin sensitivity and diet-induced obesity in the offspring

2007 ◽  
Vol 293 (3) ◽  
pp. R1056-R1062 ◽  
Author(s):  
Jacqueline Férézou-Viala ◽  
Anne-France Roy ◽  
Colette Sérougne ◽  
Daniel Gripois ◽  
Michel Parquet ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Maternal Diet ◽  
The Body ◽  
Female Rats ◽  
Leptin Resistance ◽  
High Fat ◽  
Leptin Sensitivity ◽  
The Impact

Epidemiological and animal studies suggest that the alteration of hormonal and metabolic environment during fetal and neonatal development can contribute to development of metabolic syndrome in adulthood. In this paper, we investigated the impact of maternal high-fat (HF) diet on hypothalamic leptin sensitivity and body weight gain of offspring. Adult Wistar female rats received a HF or a control normal-fat (C) diet for 6 wk before gestation until the end of the suckling period. After weaning, pups received either C or HF diet during 6 wk. Body weight gain and metabolic and endocrine parameters were measured in the eight groups of rats formed according to a postweaning diet, maternal diet, and gender. To evaluate hypothalamic leptin sensitivity in each group, STAT-3 phosphorylation was measured in response to leptin or saline intraperitoneal bolus. Pups exhibited similar body weights at birth, but at weaning, those born to HF dams weighed significantly less (−12%) than those born to C dams. When given the HF diet, males and females born to HF dams exhibited smaller body weight and feed efficiency than those born to C dams, suggesting increased energy expenditure programmed by the maternal HF diet. Thus, maternal HF feeding could be protective against adverse effects of the HF diet as observed in male offspring of control dams: overweight (+17%) with hyperleptinemia and hyperinsulinemia. Furthermore, offspring of HF dams fed either C or HF diet exhibited an alteration in hypothalamic leptin-dependent STAT-3 phosphorylation. We conclude that maternal high-fat diet programs a hypothalamic leptin resistance in offspring, which, however, fails to increase the body weight gain until adulthood.

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