PSVI-4 Effect of phytase on mineral digestibility and growth performance of pigs fed diets with pharmacological levels of Zn

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 400-401
Author(s):  
Charmaine D Espinosa ◽  
Deepak Velayudhan ◽  
Yueming Dersjant-Li ◽  
Janet Remus ◽  
Hans-Henrik H Stein

Abstract An experiment was conducted to test the hypothesis that increasing levels of phytase increases mineral digestibility and growth performance of pigs fed diets with 3,000 mg/kg of Zn. A positive control (PC) diet that met nutrient requirements and a negative control (NC) diet that was deficient in total Ca (-0.16%), digestible P (-0.14%), metabolizable energy (-50 kcal/kg), and digestible amino acids (-0.02%) were formulated. Eight additional diets were formulated by adding 500, 1,000, 1,500, or 2,000 phytase units/kg of a novel bacterial 6-phytase (PhyG) or a commercial Buttiauxella phytase (PhyB) to the NC diet. A randomized complete block design with 400 weanling pigs (5.82 ± 0.70 kg), 10 diets, 4 pigs per pen, and 10 replicate pens per diet was used. Pigs were fed the experimental diets for 28 d. Fecal samples were collected from 1 pig per pen on d 26 to 28. Linear and quadratic effects of PhyG and PhyB were determined using polynomial contrast statements. Contrast statements were used to compare effects of PC diet with NC diet, and to compare effects of PhyG diets with PhyB diets. Overall, pigs fed the NC diet had reduced growth performance (P < 0.05) compared with pigs fed the NC diet (Table 1). Linear (P < 0.01) and quadratic (P < 0.05) increases in overall average daily gain and average daily feed intake of pigs were observed as dietary concentrations of PhyG or PhyB increased. Increasing levels of PhyG or PhyB linearly increased (P < 0.01) the apparent total tract digestibility (ATTD) of P and Ca. PhyG increased ATTD of P more (P < 0.05) than PhyB. In conclusion, both phytases are effective in increasing mineral digestibility and growth performance in pigs fed diets with pharmacological levels of Zn; however, PhyG improved P digestibility more than PhyB.

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 141-142
Author(s):  
Dathan Smerchek ◽  
Zachary K Smith

Abstract Two-hundred and forty English × Continental beef steers (initial BW=365 kg [SD 22.5]) were used in a randomized complete block design to evaluate the effects of bedding application during a 36 d feedlot receiving phase on growth performance and efficiency during winter. Steers were allotted to 30 pens (n = 8 steers/pen) at the Ruminant Nutrition Center in Brookings, SD and pens were assigned to 1 of 2 treatments: 1) No bedding applied (NO) or 2) 1.8 kg (as-is basis) of wheat straw bedding/steer/d (BED). Due to the logistics of acquiring, weighing, and timely feeding of steers, the first 9 pen replicates began on test 14 d prior (begin: January 15, 2019 and end: February 20, 2019) to the last 6 pen replicates (begin: January 29, 2019 and end: March 6, 2019). Pen was the experimental unit; an α of 0.05 determined significance. Daily ambient temperature (n = 50) averaged -14.7°C [SD 5.63] and wind-chill (n = 50) averaged -17.7°C [SD 6.40]. Initial BW (365 vs. 365 ± 0.5 kg) and dry matter intake (8.19 vs. 8.22 ± 0.047 kg) did not differ (P ≥ 0.57) between NO and BED. End BW was greater for BED (P = 0.01; 419 vs. 402 ± 1.09 kg) compared to NO. Steers from BED had increased average daily gain (P = 0.01) by 48.0% and gain:feed (P = 0.01) by 49.2% over NO. Using tabular ingredient energy values and observed steer performance shrunk 4%, relative adjustments to metabolic rate were calculated. Estimated metabolic rate was elevated (P = 0.01) for NO (0.146 vs. 0.104 ± 0.0032 Mcal/BW0.75, kg). Bedding improves feedlot receiving phase growth and efficiency in eastern South Dakota during the winter. Under the environmental conditions of this experiment, steers have a 40.4% increased metabolic rate when bedding is not used and steers in bedded pens had a 35.1% increase in metabolic rate compared to (0.077 Mcal/BW0.75, kg).


2008 ◽  
Vol 99 (3) ◽  
pp. 682-690 ◽  
Author(s):  
Oluyinka A. Olukosi ◽  
Aaron J. Cowieson ◽  
Olayiwola Adeola

Energy utilization in broilers as influenced by supplementation of enzymes containing phytase or carbohydrase activities was investigated. Day-old male broilers (480) were allocated to four slaughter groups, thirty broilers in the initial slaughter group and 150 broilers in each of the final slaughter groups on days 7, 14 and 21. Broilers in each of the final slaughter groups were allocated to five treatments in a randomized complete block design, each treatment had six replicate cages of five broilers per replicate cage. The diets were maize–soyabean based with wheat as a source of NSP. The treatments were: (1) positive control that met nutrient requirements of the day-old broiler chick; (2) negative control (NC) deficient in metabolizable energy and P; (3) NC plus phytase added at 1000 FTU/kg; (4) NC plus cocktail of xylanase, amylase and protease (XAP); and (5) NC plus phytase and XAP. Gain and gain:food were depressed (P < 0·05) in the NC diet. Phytase improved (P < 0·05) gain at all ages and gain:food at days 0–14 and days 0–21. There was improvement (P < 0·01) in net energy for production, energy retained as fat and protein from days 0 to 14 and from days 0 to 21 in phytase-supplemented diet compared with the NC diet. Net energy for production was more highly correlated with performance criteria than metabolizable energy and may be a more sensitive energy utilization response criterion to use in evaluating broiler response to enzyme supplementation.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 69-70
Author(s):  
Kevin S Jerez Bogota ◽  
Christian Sanchez ◽  
Jimena Ibagon ◽  
Maamer Jlali ◽  
Pierre Cozannet ◽  
...  

Abstract Objective was to determine the effects of supplementing corn-soybean meal-based diets with a multi-enzyme on growth performance and apparent total tract digestibility (ATTD) of nutrients in growing pigs. A total of 276 pigs (BW = 33.99 ± 4.3 kg) were housed by sex in 45 pens of 6 or 7 pigs and fed 5 diets (9 pens/diet) in a randomized complete block design. Diets were positive control (PC); and negative control 1 (NC1) or negative control 2 (NC2) without or with multi-enzyme. The multi-enzyme used supplied at least 1,250, 860, 4,600, and 1,000 units of xylanase, β-glucanase, arabinofuranosidase and phytase per kilogram of diet, respectively. The PC was adequate in all nutrients according to NRC (2012) recommendations and had greater digestible P content than NC1 diet or NC2 diet by 0.134 percentage points. The NC1 diet had lower NE and digestible AA content than PC diet by 3%. The NC2 diet had lower NE and digestible AA content than PC diet by 5%. The diets were fed in 4 phases based on BW; Phase 1: 34–50 kg, Phase 2: 50–75 kg, Phase 3: 75–100 kg, and Phase 4: 100–120 kg. Nutrient digestibility was determined at the end of Phase 1. Overall (34–120 kg BW), pigs fed the PC and NC1 diets did not differ in ADG and ADFI. Pigs fed NC2 diet had lower (PPPP > 0.10). Supplemental multi-enzyme increased (P = 0.047) the ATTD of GE for NC2 diet. In conclusion, the NE and digestible AA and P can be lowered by ≤ 5% in multi-enzyme-supplemented diets without effects on growth performance pigs.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 49-50
Author(s):  
Carly M Rundle ◽  
Yueming Dersjant-Li ◽  
Barthold Hillen ◽  
Maryane S Sespere Faria Oliveira ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that a Buttiauxella phytase expressed in T. reesei can compensate the reduction in standardized total tract digestible (STTD) P, Ca, digestible energy (DE), and standardized ileal digestible (SID) AA in diets for pigs without impaired growth performance. A positive control diet (PC) was formulated to meet the requirement for STTD P and total Ca (0.33% STTD P and 0.60% Ca). A negative control diet (NC) was formulated with 0.18% STTD P and 0.45% Ca and with reduction of DE by 43 kcal/kg and SID AA by 0.01–0.02% vs. PC. Adding 250, 500, or 1,000 phytase units (FTU) per kg to the NC diet for a total of 5 experimental diets formulated three additional diets. A total of 155 pigs (BW: 8.77 ± 1.38 kg) were allotted to the 5 treatments, and there were 8 replicate pens per treatment. The experiment was conducted for 21 d. Pigs were weighed at the beginning and conclusion of the experiment, and daily feed allotments were recorded. Average daily gain (ADG), average daily feed intake (ADFI), and gain: feed ratio (G:F) were calculated for each treatment. Data were analyzed using contrast statements to compare PC and NC, PC and phytase, and to determine linear and quadratic effects of phytase. Results indicated that pigs fed the NC diet had reduced (P &lt; 0.05) ADG and G:F compared with pigs fed the PC diet (Table 1). Adding phytase to NC increased ADFI (linear, P &lt; 0.05) and ADG (quadratic, P &lt; 0.05) with the greatest values for the 1,000 FTU treatment. Including phytase in the diet also resulted in a quadratic increase (P &lt; 0.05) in G:F. In conclusion, Buttiauxella phytase may be included in diets with reduced nutrients and energy for weanling pigs without compromising pig growth performance.


2016 ◽  
Vol 96 (2) ◽  
pp. 243-251 ◽  
Author(s):  
H. Lu ◽  
A. Preynat ◽  
V. Legrand-Defretin ◽  
P.A. Geraert ◽  
O. Adeola ◽  
...  

Effect of carbohydrases and phytase supplementation on growth performance, nutrient utilization and gut health of nursery pigs was evaluated. Pigs were blocked by body weight (BW) and sex and allocated to four treatments. Treatments were a positive control (PC), a negative control (NC) deficient in metabolizable energy (ME), crude protein (CP), Ca, and non-phytate P (nPP), NC plus Rovabio® Max AP enzyme mix, at 0.05 and 0.075 g kg−1. Apparent total tract digestibility (ATTD) was determined in faecal samples. Apparent ileal digestibility (AID) was determined in ileal digesta samples collected after euthanasia. Lower final BW and average daily gain (ADG) (P < 0.05) were observed in NC compared with PC. Enzyme at 0.05 g kg−1increased (P < 0.05) BW on d 14 and d 41, respectively, and also increased ADG. Enzyme at 0.075 g kg−1increased BW on d 14 and ADG on d 0 to d 14 (P < 0.05). Feed efficiency [gain to feed ratio (G:F)] was greater (P < 0.05) in PC than NC from d 15 to d 41 and from d 0 to d 41. No difference in G:F was observed with enzyme supplementation. Higher (P < 0.05) serum Ca and bone ash were observed in PC than NC. Enzyme increased the ATTD of Ca and P (P < 0.05) compared with NC.


Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1766
Author(s):  
Dathan Thomas Smerchek ◽  
Zachary Kidd Smith

Two randomized complete block design experiments were conducted to evaluate the effect of bedding use in confined beef steers. Experiment 1 used Simmental × Angus steers (n = 240; initial body weight (BW) = 365 ± 22.5 kg). Experiment 2 used newly weaned Charolais × Red Angus steers (n = 162; initial BW = 278 ± 13.4 kg). Steers were allotted to one of two treatments: (1) no bedding (NO), or (2) 1.8 kg (Experiment 1) or 1.0 kg (Experiment 2) of wheat straw (as-is basis) bedding/steer·d−1 (BED). In Experiment 1, applying bedding improved (p ≤ 0.01) dry matter intake (DMI), kg of gain to kg of feed (G:F), and average daily gain (ADG). Bedding reduced (p = 0.01) the estimated maintenance coefficient (MQ). Dressing percentage, rib fat, marbling, and yield grade were increased (p ≤ 0.03) in NO. Bedding resulted in an increase (p = 0.01) in serum insulin-like growth factor I (IGF-I). In Experiment 2, a tendency (p = 0.06) for increased DMI for NO was noted. Bedding improved G:F (p = 0.01). MQ was elevated (p = 0.03) for NO and NO had an increase (p = 0.02) in serum concentration of urea-N (SUN). An increase (p = 0.01) in serum non-esterified fatty acid was noted for NO. These data indicate that bedding application should be considered to improve growth performance and feed efficiency by reducing maintenance energy requirements in beef steers during the feedlot receiving and finishing phase.


Author(s):  
S. Gilani ◽  
M.I. Garcia ◽  
L. Barnard ◽  
Y. Dersjant-Li ◽  
C. Millán ◽  
...  

The following study evaluated effects of a xylanase and beta-glucanase combination on growth performance of broilers fed energy reduced versus nutritionally adequate maize-soybean meal-based diets. A total of 648, one-day-old male broilers (Ross 308) were assigned to floor-pens (24 birds/pen, nine pens/treatment, three treatments) in a randomised block design. Treatments included: (1) a nutritionally adequate positive control diet (PC); (2) a negative control (NC) diet in which energy, crude protein and digestible amino acids were reduced by 3.4% (-105 kcal apparent metabolisable energy), 2.3% and 1.2 to 3.0% vs PC, respectively; and (3) NC plus a xylanase and beta-glucanase combination that supplied 1,220 U xylanase and 152 U beta-glucanase per kilogram of final feed. All diets contained a background of 500 FTU/kg phytase and were offered to birds ad libitum. Birds fed NC showed reduced average daily gain (ADG) by -6.1% (P<0.05); increased feed conversion ratio (FCR) by 9.2 points (P<0.05), and overall (d 1-35) body weight corrected FCR which was increased by 9.4 points (P<0.05) vs the PC group. Enzyme supplementation increased final BW (+4.2%, P<0.05), ADG (+5.4%, P<0.05) and tended to reduce FCR (+7.5 points, P=0.054) from d 22-35 vs NC, without affecting average daily feed intake. Improvements in performance due to the enzyme combination were equivalent to performance on the PC diet in all cases. The results suggested that significant improvements in growth performance of broilers fed maize-soybean meal-based diets which had been reduced in energy and nutrients can be realised by supplementation with xylanase in combination with beta-glucanase.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 56-57
Author(s):  
Ning Lu ◽  
Carine Vier ◽  
Wayne Cast ◽  
Uislei Orlando ◽  
Dan Hamilton ◽  
...  

Abstract The objective was to determine the effects of different standardized ileal digestible (SID) lysine (Lys) specifications on performance of grow-finish pigs. A total of 2,106 pigs (PIC 359×Camborough, initially 22.0±0.43 kg) were used in a 72-d growth trial with 27 pigs per pen and 13 pens per treatment in a randomized complete block design. Pigs were fed corn-soybean-meal-based diets in three phases from 22-41, 41-62, and 62-91 kg body weight (BW). Treatments consisted of low (L) or high (H) SID Lys in each phase: LLL (1.05, 0.88, and 0.73%); LLH (1.05, 0.88, and 0.82%); LHH (1.05, 0.99, and 0.82%); HLL (1.18, 0.88, and 0.73%); HHL (1.18, 0.99, and 0.73%); and HHH (1.18, 0.99, and 0.82%). Lysine curves from PIC2008 and PIC2016 nutrient specification manuals were used to estimate SID Lys levels for L or H diets, respectively. Data were analyzed using generalized linear mixed models with pen as the experimental unit. From 22-41 kg, feeding high Lys improved (P&lt; 0.10) average daily gain (ADG), feed efficiency (G:F), and final BW compared to low Lys. From 22-62 kg, feeding HH Lys increased (P&lt; 0.05) ADG compared feeding LL or HL Lys. Pigs fed HH Lys or LH Lys had improved (P&lt; 0.05) G:F compared to those fed LL Lys. Pigs fed LH or HH Lys had greater (P&lt; 0.05) final BW compared to pigs fed HL. Pigs fed HH Lys had greater (P&lt; 0.05) feed intake compared to pigs fed HL. From 22-91 kg, there was no evidence for differences in ADG, feed intake, G:F, or final BW. In summary, pigs fed 1.18% SID Lys had improved ADG, G:F, and final BW compared to pigs fed 1.05% SID Lys from 22-41 kg. Feeding a range of Lys specifications resulted in similar growth performance from 22-91 kg.


2020 ◽  
Vol 8 (2) ◽  
pp. 49-59
Author(s):  
Y. Dersjant-Li ◽  
K. Van de Belt ◽  
C. Kwakernaak ◽  
L. Marchal

The effect of down specifying nutrients in diets supplemented with Buttiauxella spp. phytase was studied in a commercial trial. Three treatments were tested with five replicate groups, each containing 700, one-day-old straight run Ross 308 broilers. Birds were fed pelleted diets from days 0-42 in four phases: starter (days 0-10); grower (days 11-21); finisher 1 (days 22-35) and finisher 2 (days 36-42). A nutritionally adequate, unsupplemented, positive control (PC) diet based on wheat, corn and soybean meal was compared against two down specified, negative control (NC) diets containing Buttiauxella phytase supplemented at 500 or 1000 FTU/kg. The reduction level was 0.134 and 0.159% unit for digestible phosphorus, 0.164 and 0.189% unit for calcium, 0.03 and 0.04% unit for sodium, 0.283 and 0.309 MJ/kg for nitrogen corrected apparent metabolizable energy in all phases and variable digestible amino acids in different phases, respectively for the diets containing the phytase at 500 and 1000 FTU/kg. An unsupplemented NC diet was not included, as it would have caused welfare and health issues. Weight gain and mortality-corrected feed conversion ratio for birds receiving phytase at either inclusion levels were equivalent to the PC group. Feed intake was increased by 500 FTU/kg phytase (P<0.05) during 0-21 d vs PC. Including 1000 FTU/kg phytase reduced water intake vs PC at 0-42 d and water-to-feed intake ratios, after the starter phase (P<0.05). Carcass yield in birds supplemented with either phytase level was not different from PC. Tibia ash was unaffected by treatment. Estimated feed costs (inclusive of phytase) were lower in supplemented than un-supplemented (PC) diets, by 10.0 to 13.7 €/ton diet. The trial demonstrated that reducing nutrient specifications of diets supplemented with Buttiauxella phytase maintained growth performance, lowered feed costs, with production benefits maximised at inclusion levels of 1000 FTU/kg.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 196-197
Author(s):  
Marley Conceição dos Santos ◽  
Uislei A Orlando ◽  
Carine M Vier ◽  
Leopoldo M Almeida ◽  
Ning Lu ◽  
...  

Abstract A total of 400 mixed parity sows (PIC Camborough, Hendersonville, TN) were used to evaluate the effects of increasing soybean oil supplementation prior to farrowing on sow and litter performance under commercial conditions. On d 111 of gestation, females were transferred to the farrowing room, blocked by parity and body weight, and allotted to one of four treatments within block in a randomized complete block design. Treatments included different amounts of soybean oil supplementation from d 111 to 113 of gestation, which included: 1) 0 g; 2) 250 g; 3) 500 g; 4) 1000 g. A corn-soybean meal-based diet containing 3.06% crude fat was fed and treatments were top-dressed at an equal amount for at least 3 d to achieve the designated supplementation level. The experimental data were analyzed using generalized linear mixed models with the GLIMMIX procedure of SAS. Polynomial contrasts were implemented to evaluate the linear and quadratic effects of increasing soybean oil supplementation. Response variables analyzed as normally distributed included sow body weight (BW) and caliper score at weaning, BW and caliper changes during lactation, piglet weight at birth, 24h after birth, and at weaning, colostrum yield, and litter average daily gain from cross-foster to weaning. Total born was analyzed following a poisson distribution and percentage born alive, stillbirth, and pre-weaning survivability were analyzed following a binomial distribution. Covariates were used if they significantly improved the model fit. Stillbirth rate increased (quadratic, P&lt; 0.05) and percentage born alive marginally reduced (quadratic, P&lt; 0.10) with increasing soybean oil supplementation up to 500 g, and then reduced and increased at 1000 g. There was no evidence (P &gt;0.10) for linear or quadratic treatment effects for any of the other response variables evaluated. In conclusion, results from this study did not support supplementing increasing levels of soybean oil for sows prior to farrowing.


Sign in / Sign up

Export Citation Format

Share Document