scholarly journals Mitochondrial NADH- or NADPH-Linked Aquacobalamin Reductase Activity Is Low in Human Skin Fibroblasts with Defects in Synthesis of Cobalamin Coenzymes

1996 ◽  
Vol 126 (12) ◽  
pp. 2947-2951 ◽  
Author(s):  
Fumio Watanabe ◽  
Hisako Saido ◽  
Ryoichi Yamaji ◽  
Kazutaka Miyatake ◽  
Yuji Isegawa ◽  
...  
Keyword(s):  
Human Skin ◽  
Reductase Activity ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Effects of β-Blockers on 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Activity in Cultured Human Skin Fibroblasts

1991 ◽  
Vol 19 (12) ◽  
pp. 1057-1065
Author(s):  
Hiroshi YOSHIDA ◽  
Michio SUZUKAWA ◽  
Hiroshi HOSOAI ◽  
Hideki SHIGE ◽  
Kouji TOMIYASU ◽  
...  
Keyword(s):  
Human Skin ◽  
Coenzyme A ◽  
Reductase Activity ◽  
Beta Blockers ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Coenzyme A Reductase

Cholesterol-mediated regulation of HMG-CoA reductase in microsomes from human skin fibroblasts and rat liver

1990 ◽  
Vol 68 (3) ◽  
pp. 674-679 ◽  
Author(s):  
R. George ◽  
P. J. Davis ◽  
L. Luong ◽  
M. J. Poznansky
Keyword(s):  
Tissue Culture ◽  
Enzyme Activity ◽  
Human Skin ◽  
Rat Liver ◽  
Reductase Activity ◽  
Cholesterol Content ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Hmg Coa Reductase ◽  
Coa Reductase

3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase activity was determined in microsomes from human skin fibroblasts and rat liver that had been variously manipulated in vivo or in tissue culture to up- and down-regulate the enzyme. The cholesterol content of these microsomal preparations was then altered by depletion to or enrichment from either cholesterol-free or cholesterol-rich lipid vesicles. Microsomes from human skin fibroblasts responded to cholesterol depletion by increasing HMG-CoA reductase activity and by decreasing it in response to cholesterol enrichment. This was independent of the initial enzyme activity or the tissue culture conditions. Alterations in cholesterol content of rat liver microsomes in vitro failed to demonstrate any significant changes in HMG-CoA reductase activity whether the microsomes started with low enzyme activity (cholesterol-fed rats) or with high enzyme activity (cholestyramine-treated rats). The results are discussed in relation to previously published data and in respect to differences in the control of the human skin fibroblast and rat liver enzymes.Key words: cholesterol, HMG-CoA reductase, microsomes, fibroblasts, rat liver.

117-LB: Nonviral Direct Reprogramming of Human Skin Fibroblasts into Hormone-Expressing ß-Like Cell Clusters

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 117-LB
Author(s):  
LUKE R. LEMMERMAN ◽  
MARIA ANGELICA RINCON-BENAVIDES ◽  
SARAH A. TERSEY ◽  
BRITANI N. BLACKSTONE ◽  
HEATHER M. POWELL ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Direct Reprogramming ◽  
Human Skin Fibroblasts ◽  
Cell Clusters

Protective Effects of Green Tea Seed Extract against UVB-irradiated Human Skin Fibroblasts

2014 ◽  
Vol 43 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Ok Kyung Kim ◽  
Da-Eun Nam ◽  
Min-Jae Lee ◽  
Namgil Kang ◽  
Jae-Youn Lim ◽  
...  
Keyword(s):  
Human Skin ◽  
Green Tea ◽  
Seed Extract ◽  
Skin Fibroblasts ◽  
Protective Effects ◽  
Human Skin Fibroblasts

The intralysosomal pH in cultured human skin fibroblasts in relation to cystine accumulation in patients with cystinosis

1983 ◽  
Vol 116 (1) ◽  
pp. 154-161 ◽  
Author(s):  
Ronald P.J. Oude Elferink ◽  
Erik Harms ◽  
Anneke Strijland ◽  
Joseph M. Tager
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

1984 ◽  
Vol 12 (2) ◽  
pp. 89-97
Author(s):  
Graham R. Elliott ◽  
H.E. Amos ◽  
James W. Bridges
Keyword(s):  
Human Skin ◽  
Psoriasis Patient ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Cell Type ◽  
Normal Human Skin ◽  
Rate Of Growth ◽  
Structural Analogues ◽  
Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

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