scholarly journals Early host cell reactivation of an oxidatively damaged adenovirus-encoded reporter gene requires the Cockayne syndrome proteins CSA and CSB

Mutagenesis ◽  
2010 ◽  
Vol 26 (2) ◽  
pp. 315-321 ◽  
Author(s):  
D. M. Leach ◽  
A. J. Rainbow
Keyword(s):  
Host Cell ◽  
Reporter Gene ◽  
Cockayne Syndrome ◽  
Cell Reactivation ◽  
Host Cell Reactivation

Depletion of poly(ADP-ribose) polymerase-1 reduces host cell reactivation of a UV-damaged adenovirus-encoded reporter gene in human dermal fibroblasts

DNA Repair ◽  
2008 ◽  
Vol 7 (4) ◽  
pp. 617-632 ◽  
Author(s):  
Medini M. Ghodgaonkar ◽  
Natalie Zacal ◽  
Shaqil Kassam ◽  
Andrew J. Rainbow ◽  
Girish M. Shah
Keyword(s):  
Host Cell ◽  
Reporter Gene ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Cell Reactivation ◽  
Host Cell Reactivation

Pre-UV-Treatment of Cells Results in Enhanced Host Cell Reactivation of a UV Damaged Reporter Gene in CHO-AA8 Chinese Hamster Ovary Cells but Not in Transcription-Coupled Repair Deficient CHO-UV61 Cells

2004 ◽  
Vol 24 (6) ◽  
pp. 559-576 ◽  
Author(s):  
Lili Liu ◽  
Andrew J. Rainbow
Keyword(s):  
Host Cell ◽  
Reporter Gene ◽  
Chinese Hamster Ovary ◽  
Excision Repair ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Cell Reactivation ◽  
Host Cell Reactivation ◽  
Transcription Coupled Repair

We have used a non-replicating recombinant adenovirus, Ad5MCMVlacZ, which expresses the β-galactosidase reporter gene, to examine both constitutive and inducible repair of UV-damaged DNA in repair proficient CHO-AA8 Chinese hamster ovary cells and in mutant CHO-UV61 cells which are deficient in the transcription-coupled repair (TCR) pathway of nucleotide excision repair. Host cell reactivation (HCR) of β-galactosidase activity for UV-irradiated Ad5MCMVlacZ was significantly reduced in non-irradiated CHO-UV61 cells compared to that in non-irradiated CHO-AA8 cells suggesting that repair in the transcribed strand of the UV-damaged reporter gene in untreated cells utilizes TCR. Prior UV-irradiation of cells with low UV fluences resulted in a transient enhancement of HCR for expression of the UV-damaged reporter gene in CHO-AA8 cells but not in TCR deficient CHO-UV61 cells. These results suggest the presence of an inducible DNA pathway in CHO cells that results from an enhancement of TCR or a mechanism that involves the TCR pathway.

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