scholarly journals Two-step total gene synthesis method

2004 ◽  
Vol 32 (7) ◽  
pp. e59-e59 ◽  
Author(s):  
L. Young
Keyword(s):  
Synthesis Method ◽  
Gene Synthesis

scholarly journals An improved gene synthesis method with asymmetric directions of oligonucleotides designed using a simulation program

BioTechniques ◽  
2020 ◽  
Vol 69 (3) ◽  
pp. 211-219
Author(s):  
Kotetsu Kayama ◽  
Hibiki Hashizume ◽  
Gerry Amor Camer ◽  
Daiji Endoh
Keyword(s):  
Dna Synthesis ◽  
Synthesis Method ◽  
Simulation Program ◽  
Reverse Direction ◽  
Gene Synthesis ◽  
Overlap Extension Pcr ◽  
Overlap Extension ◽  
Efficiency And Effectiveness ◽  
Number Of Cycles ◽  
Artificial Gene

Artificial gene synthesis based on oligonucleotide augmentation is known as overlap extension PCR which generates a variety of intermediate synthetic products. The orientation and concentration of oligomers can be adjusted to reduce the synthesis of intermediates and optimize the full-length process of DNA synthesis, using a simulation program for serial oligomer extension. The efficiency of the serial oligomer extension process is predicted to be greatest when oligomers are in a ‘forward-reverse-reverse-reverse’ direction. Oligomers with such designed directions demonstrated generation of the desired product in the shortest time (number of cycles) by repeated annealing and elongation. This method, named Asymmetric Extension supported by a Simulator for Oligonucleotide Extension (AESOE), has shown efficiency and effectiveness with potentials for future improvements and optimal usage in DNA synthesis.

A simple, universal, efficient PCR-based gene synthesis method: Sequential OE-PCR gene synthesis

Gene ◽  
2013 ◽  
Vol 524 (2) ◽  
pp. 347-354 ◽  
Author(s):  
Pingping Zhang ◽  
Yingying Ding ◽  
Wenting Liao ◽  
Qiuli Chen ◽  
Huaqun Zhang ◽  
...  
Keyword(s):  
Synthesis Method ◽  
Gene Synthesis

scholarly journals Integrating Gene Synthesis and Microfluidic Protein Analysis for Rapid Protein Engineering

2015 ◽  
Author(s):  
Matthew C Blackburn ◽  
Ekaterina Petrova ◽  
Bruno E Correia ◽  
Sebastian Josef Maerkl
Keyword(s):  
Protein Design ◽  
Solid Phase ◽  
Synthesis Method ◽  
Gene Synthesis ◽  
Sequence Specificity ◽  
Quantitative Characterization ◽  
Synthetic Genes ◽  
In Silico Modeling ◽  
On Chip ◽  
Protein Biophysics

The capability to rapidly design proteins with novel functions will have a significant impact on medicine, biotechnology, and synthetic biology. Synthetic genes are becoming a commodity, but integrated approaches have yet to be developed that take full advantage of gene synthesis. We developed a solid-phase gene synthesis method based on asymmetric primer extension (APE) and coupled this process directly to high-throughput, on-chip protein expression, purification, and characterization (mechanically induced trapping of molecular interactions, MITOMI). By completely circumventing molecular cloning and cell-based steps, APE-MITOMI reduces the time between protein design and quantitative characterization to 3-4 days. With APE- MITOMI we synthesized and characterized over 440 zinc-finger (ZF) transcription factors (TF), showing that although ZF TFs can be readily engineered to recognize a particular DNA sequence, engineering the precise binding energy landscape remains challenging. We also found that it is possible to engineer ZF – DNA affinity precisely and independently of sequence specificity and that in silico modeling can explain some of the observed affinity differences. APE-MITOMI is a generic approach that should facilitate fundamental studies in protein biophysics, and protein design/engineering.

Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program

2013 ◽  
pp. 9-17 ◽  
Author(s):  
Gang Li ◽  
Bing-Xue Dong ◽  
Yu-Huan Liu ◽  
Chang-Jie Li ◽  
Li-Ping Zhang
Keyword(s):  
Synthesis Method ◽  
Gene Synthesis ◽  
Overlap Extension Pcr ◽  
Overlap Extension

Systematic targeted gene deletion using the gene-synthesis method in fission yeast

2014 ◽  
Vol 106 ◽  
pp. 72-77 ◽  
Author(s):  
Miyoung Nam ◽  
Sook-Jeong Lee ◽  
Sangjo Han ◽  
Dongsup Kim ◽  
Minho Lee ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Gene Deletion ◽  
Synthesis Method ◽  
Gene Synthesis ◽  
Targeted Gene Deletion

Quikgene: A gene synthesis method integrated with ligation-free cloning

2011 ◽  
Vol 415 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Yanjun Mao ◽  
Juanyu Lin ◽  
Aibin Zhou ◽  
Kunmei Ji ◽  
Jennifer S. Downey ◽  
...  
Keyword(s):  
Synthesis Method ◽  
Gene Synthesis
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