P0473LONGITUDINAL CHANGES OF IGA1 O-GLYCOFORM IN IGA NEPHROPATHY

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
YUKAKO OHYAMA ◽  
Hisateru Yamaguchi ◽  
Kazuki Nakajima ◽  
Daijo Inaguma ◽  
Midori Hasegawa ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Molecular Level ◽  
High Resolution Mass Spectrometry ◽  
Neuraminidase Treatment ◽  
Time Points ◽  
Serum Iga ◽  
High Throughput Method ◽  
Before And After

Abstract Background and Aims Elevation of circulatory IgA1 with galactose-deficient (Gd) hinge-region (HR) O-glycans (Gd-IgA1) has been detected in most IgA nephropathy (IgAN) patients based on lectin ELISA. However, new approaches are needed for molecular-level characterization of IgA1 HR glycoform(s) in IgAN. We established a high-throughput method for analysis of IgA1 HR O-glycoforms using liquid chromatography-high-resolution mass spectrometry (LC-HRMS). To identify IgAN-associated IgA1 HR O-glycoforms and to assess their changes after therapy (with or without corticosteroids (CS)), we profiled IgA1 HR glycopeptides from sera collected at two time points (before and after therapy) from Japanese IgAN patients. Method Of the 10 Japanese IgAN patients recruited, 4 received CS treatment (CS group) and 6 have not (non-CS group). Japanese healthy volunteers (HC, n=10) were recruited as controls. Serum IgA1 was purified by affinity chromatography from HC and IgAN patients before and after therapy. After neuraminidase treatment and trypsin digestion, IgA1 HR glycosylation heterogeneity was analyzed by LC-HRMS. The relative abundance (RA, %) for each glycopeptide was calculated as percentage to the total IgA1 HR glycopeptide. The amount of each glycopeptide was then calculated by multiplying serum IgA concentration (mg/dL) by RA. Results Approximately 60% of IgA1 HR O-glycoforms in IgAN patients and HC were Gd O-glycoforms; these glycoforms contained one to three Gd-glycan(s), designated as 1 Gd-glycoform, 2 Gd-glycoform and 3 Gd-glycoform, respectively. In IgAN patients, the RA of non Gd-IgA1 glycoforms was elevated (P=0.002) and correlated with proteinuria (g/gCr) at renal biopsy (P=0.039, R=0.657). The amounts of non Gd- and 1Gd-glycoforms were higher in IgAN patients compared to HC (each P<0.001). After several years of follow up (2.77 years (1.44-3.85)), the RA of non Gd-glycoforms decreased in CS group of IgAN patients (P=0.039) whereas it remained unchanged in the non-CS group (P=0.488). The amount of non Gd-glycoforms exhibited similar trends, i.e., decreased in CS group (P=0.068) whereas it remained unchanged in the non-CS group (P=0.943). Conclusion This study profiled serum IgA1 for IgAN-associated IgA1 HR O-glycoforms at the molecular level and assessed their changes in response to CS vs. non-CS therapy. IgA1 HR O-glycoforms altered by treatment may serve as a biomarker(s) for monitoring patients’ responses to therapy.

Characterization of health complaints before and after removal of amalgam fillings — 3-year follow-up

2012 ◽  
Vol 71 (3-4) ◽  
pp. 560-569 ◽  
Author(s):  
Gunvor Bentung Lygre ◽  
Therese Thornton Sjursen ◽  
Johanna Svahn ◽  
Vigdis Helland ◽  
Birgitte Fos Lundekvam ◽  
...  
Keyword(s):  
Health Complaints ◽  
Before And After ◽  
Amalgam Fillings

scholarly journals Clinical efficacy of single intraoperative 500 mg methylprednisolone management therapy for thoracic myelopathy caused by ossification of the ligamentum flavum

2020 ◽  
Author(s):  
Xiaoyang Huo ◽  
Jiaming Zhou ◽  
Shiwei Liu ◽  
Xing Guo ◽  
Yuan Xue
Keyword(s):  
Posterior Approach ◽  
Ligamentum Flavum ◽  
Nerve Function ◽  
Thoracic Myelopathy ◽  
Time Points ◽  
Before And After ◽  
Long Term Follow Up ◽  
Frankel Grade

Abstract Background The objective of our study was to compare clinical outcome and postoperative complications between patients with thoracic myelopathy caused by ossification of the ligamentum flavum (OLF) treated with and without intraoperative methylprednisolone (MP).Methods This retrospective study enrolled 101 patients who underwent posterior approach surgery for OLF and were followed up at least 1 year. Patients were divided into two groups according to MP use in the operation: MP group (n=47) and non-MP group (n=54). Clinical outcomes and complications were evaluated before and after operation and at the last follow-up.Results Significant differences were found in modified Japanese Orthopedics Association (mJOA) scores and proportion of Frankel grade (A-C) between the two groups immediately after surgery and at 2-week follow-up. No significant differences were found between the two groups in mJOA score before operation and at the final follow-up. Moreover, no significant differences were observed in recovery rate according to mJOA score at any time points, and there were no significant differences in the proportion of Frankel grade between the two groups. There were 13 documented infections: 10 in the MP group and 3 in the non-MP group ( P =0.034).Conclusion Management therapy with intraoperative 500 mg methylprednisolone promoted the recovery of nerve function within 2 weeks in patients with thoracic myelopathy caused by OLF. However, long-term follow-up results showed that intraoperative methylprednisolone was inefficient. Moreover, intraoperative methylprednisolone increased the rate of wound infection.

scholarly journals Clinical efficacy of single intraoperative 500 mg methylprednisolone management therapy for thoracic myelopathy caused by ossification of the ligamentum flavum

2020 ◽  
Author(s):  
Xiaoyang Huo ◽  
Jiaming Zhou ◽  
Shiwei Liu ◽  
Xing Guo ◽  
Yuan Xue
Keyword(s):  
Posterior Approach ◽  
Ligamentum Flavum ◽  
Thoracic Myelopathy ◽  
Time Points ◽  
Significant Difference ◽  
Before And After ◽  
Long Term Follow Up ◽  
Frankel Grade

Abstract Background: The objective of our study was to compare clinical outcome and postoperative complications between patients with thoracic myelopathy caused by ossification of the ligamentum flavum (OLF) treated with and without intraoperative methylprednisolone (MP).Methods: This retrospective study enrolled 101 patients who underwent posterior approach surgery for OLF and were followed up at least 1 year. Patients were divided into two groups according to MP use in the operation: MP group (n=47) and non-MP group (n=54). Clinical outcomes and complications were evaluated before and after operation and at the last follow-up. Results: Significant differences were found in modified Japanese Orthopedics Association (mJOA) scores and proportion of Frankel grade (A-C) between the two groups immediately after surgery and at 2-week follow-up. No significant differences were found between the two groups in mJOA score before operation and at the final follow-up. Moreover, no significant differences were observed in recovery rate according to mJOA score at any time points, and there was no significant difference in the proportion of Frankel grade (A-C) between the two groups at final follow-up. There were 13 documented infections: 10 in the MP group and 3 in the non-MP group (P=0.034).Conclusion: Management therapy with intraoperative 500 mg MP showed better recovery of nerve function within 2 weeks in patients with thoracic myelopathy caused by OLF compared with those did not receive MP. However, long-term follow-up results showed that there was no significant difference in neurological recovery between patients with intraoperative MP or not. Moreover, intraoperative MP increased the rate of wound infection.

scholarly journals The Influence of Overnight Orthokeratology on Ocular Surface and Meibomian Gland Dysfunction in Teenagers with Myopia

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Xiu Wang ◽  
Jing Li ◽  
Rui Zhang ◽  
Na Li ◽  
Yi Pang ◽  
...  
Keyword(s):  
Ocular Surface ◽  
Tear Film ◽  
Meibomian Gland ◽  
Meibomian Gland Dysfunction ◽  
Ocular Surface Disease Index ◽  
Time Points ◽  
Meniscus Height ◽  
Significant Difference ◽  
Before And After

Purpose. The aim of this study was to investigate the effect of overnight orthokeratology (OOK) on ocular surface and meibomian gland dysfunction in teenagers with myopia. Methods. A total of 59 subjects were recruited in this prospective study. The following tests were performed before and after 1, 3, 6, 12, and 24 months of OOK lens wear, including ocular surface disease index (OSDI) questionnaire, slit-lamp examination, and Keratograph 5M. Results. No infectious keratitis occurred during the study. OSDI scores increased gradually and reached the maximum at 6 months of OOK wear (P<0.001). The meniscus height was significantly increased at 1 and 3 months after the initiation of OOK (P=0.006, P=0.035). The corneal fluorescein staining at 1, 3, 6, 12, and 24 months after wearing OOK were all increased than the prewearing level with significant difference (P=0.014, P=0.036, P<0.001, P<0.001, and P=0.008, respectively). The first and the average tear film NIKBUT were all higher than the prewearing level, but there was no significant difference between every follow-up time points (P>0.05). The lid margin abnormalities were significantly increased (P=0.003, P=0.038, and P=0.015) at 6, 12, and 24 months after the initiation of OOK. There was no significant difference in the meibomian gland orifice scores at each follow-up time points compared to the prewearing level (P>0.05). The meibomian gland lipid secretion scores after wearing OOK were higher than those of the prewearing level, however, without statistically significant difference (P>0.05). No significant differences of the degree of difficulty of lipid excretions were detected after the initiation of OOK (P>0.05). There was no significant difference in meibomian gland dropout scores between all follow-up time points and the prewearing level (P=1.000). Conclusion. OOK increased the symptoms of dry eye and decreased the function of tear film by affecting the meniscus height and BUT. OOK did not affect the function of meibomian glands.Clinical Study registration number: ChiCTR18000185708.

Characterization of human α-galactosidase A and B before and after neuraminidase treatment

1975 ◽  
Vol 391 (2) ◽  
pp. 349-360 ◽  
Author(s):  
Giovanni Romeo ◽  
Gianfranco Di Matteo ◽  
Michelle D'Urso ◽  
Su-Chen Li ◽  
Yu-Teh Li
Keyword(s):  
Neuraminidase Treatment ◽  
Before And After

scholarly journals Biometric changes after vitrectomy with silicone oil tamponade

2021 ◽  
Vol 2 (2) ◽  
pp. 56-62
Author(s):  
Simin Hosseini ◽  
Amir Faramarzi ◽  
Siamak Moradian ◽  
Mehdi Yaseri
Keyword(s):  
Silicone Oil ◽  
Corneal Thickness ◽  
Anterior Chamber Depth ◽  
Biometric Data ◽  
Cavity Depth ◽  
Time Points ◽  
Biometric Parameters ◽  
Before And After ◽  
Silicone Oil Tamponade

Background: The Lenstar LS 900 (Haag-Streit AG, Koeniz, Switzerland) is an optical biometer, and its measurements are highly repeatable and precise in cataractous eyes. This study investigated changes in biometric parameters, including axial length (AL), anterior chamber depth (ACD), lens thickness (LT), vitreous cavity depth (VD), and central corneal thickness (CCT) before and after three-port 23-Gauge pars plana vitrectomy with silicone oil tamponade. Methods: This was a prospective follow-up study. Patients who were scheduled for surgery underwent a detailed slit-lamp examination and objective cycloplegic refraction preoperatively. In eligible cases, the Lenstar LS 900 was used to measure biometric parameters. At the 1-month postoperative follow-up, we repeated the same assessments for the silicone oil (SO)-filled eyes. Data were analyzed to assess the significance of changes and to test the possible correlation of values between the two time points. Results: Twenty-three patients with a mean ± SD age of 60 ± 12 years completed the study. Postoperatively, we found a significant increase in AL and a decrease in ACD and CCT (all P < 0.05), with no significant changes in LT and VD. A significant correlation was found for ACD, CCT, and cylinder values between the two time points (all P < 0.05). Postoperatively, the spherical and cylindrical components of refraction demonstrated a hyperopic shift, but did not change statistically significantly. Conclusions: The Lenstar LS 900 underestimated the ACD and overestimated the AL in SO-filled eyes when comparing pre- and postoperative values, in phakic as well as pseudophakic eyes. In planning for cataract surgery in this group of patients, it is more reasonable to calculate IOL power based on the biometric data of the fellow eye, although this may not eliminate possible errors. Further studies with a larger sample size, longer follow-up, and robust study design are necessary to confirm our preliminary results.

Non-paroxysmal atrial fibrillation mapping: characterization of the electrophysiological substrate using a novel integrated mapping technique

EP Europace ◽  
2019 ◽  
Vol 21 (8) ◽  
pp. 1193-1202
Author(s):  
Giuseppe Ciconte ◽  
Gabriele Vicedomini ◽  
Wenwen Li ◽  
Jan O Mangual ◽  
Luke McSpadden ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Catheter Ablation ◽  
Paroxysmal Atrial Fibrillation ◽  
Mapping Technique ◽  
Wavefront Propagation ◽  
Bipolar Voltage ◽  
Before And After ◽  
Underlying Mechanisms

Abstract Aims Clinical outcomes after radiofrequency catheter ablation (RFCA) remain suboptimal in the treatment of non-paroxysmal atrial fibrillation (AF). Electrophysiological mapping may improve understanding of the underlying mechanisms. To describe the arrhythmia substrate in patients with persistent (Pers) and long-standing persistent (LSPers) AF, undergoing RFCA, using an integrated mechanism mapping technique. Methods and results Patients underwent high-density electroanatomical mapping before and after catheter ablation. Integrated maps characterized electrogram (EGM) cycle length (CL) in regions with repetitive–regular (RR) activations, stable wavefront propagation, fragmentation, and peak-to-peak bipolar voltage. Among 83 patients (72% male, 60 ± 11 years old), RR activations were identified in 376 regions (mean CL 180 ± 31 ms). PersAF patients (n = 43) showed more RR sites per patient (5.3 ± 2.4 vs. 3.7 ± 2.1, P = 0.002) with faster CL (166 ± 29 vs. 190 ± 29 ms; P < 0.001) and smaller surface area of fragmented EGMs (15 ± 14% vs. 27 ± 17%, P < 0.001) compared with LSPersAF. The post-ablation map in 50 patients remaining in AF, documented reduction of the RR activities per patient (1.5 ± 0.7 vs. 3.7 ± 1.4, P < 0.001) and area of fragmentation (22 ± 17% vs. 8 ± 9%, P < 0.001). Atrial fibrillation termination during ablation occurred at RR sites (0.48 ± 0.24 mV; 170.5 ± 20.2 ms CL) in 31/33 patients (94%). At the latest follow-up, arrhythmia freedom was higher among patients receiving ablation >75% of RR sites (Q4 82.6%, Q3 63.1%, Q2 35.1%, and Q1 0%; P < 0.001). Conclusion The integrated mapping technique allowed characterization of multiple arrhythmic substrates in non-paroxysmal AF patients. This technique might serve as tool for a substrate-targeted ablation approach.

scholarly journals Longitudinal Study on Growth and Body Mass Index before and after Diagnosis of Childhood Craniopharyngioma

2004 ◽  
Vol 89 (7) ◽  
pp. 3298-3305 ◽  
Author(s):  
Hermann L. Müller ◽  
Angela Emser ◽  
Andreas Faldum ◽  
Gina Bruhnken ◽  
Nicole Etavard-Gorris ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Body Mass ◽  
Growth Rates ◽  
Linear Regression Analysis ◽  
Ponderal Index ◽  
Growth Impairment ◽  
Time Points ◽  
Before And After ◽  
Tumor Involvement

Abstract We analyzed whether childhood craniopharyngioma predisposes to obesity and growth impairment. Height/length, body mass index (BMI), and hypothalamic involvement were evaluated in 90 patients at standardized ages and time points before, after, and at the time of diagnosis. Relevant decreases in height sd score (SDS) started at 10–12 months of age and persisted until diagnosis of childhood craniopharyngioma. Relevant increases in BMI SDS were detectable between 4 and 5 yr of age. Postoperative BMI SDS (yr 1–6) had a weak positive correlation with BMI SDS at the time of diagnosis. In linear regression analysis, hypothalamic tumor involvement (P &lt; 0.001), ponderal index at birth (P = 0.014), and BMI SDS at age 6–7 months (P = 0.029) and at age 5 yr (P &lt; 0.001) had relevant and independent impacts on the development of obesity. Patients with hypothalamic involvement (n = 48) presented lower ponderal index and BMI SDS at birth and higher BMI SDS at the time of diagnosis (P &lt; 0.001) as well as during annual follow-up (P &lt; 0.001) compared with patients without hypothalamic involvement (n = 42). From childhood (3.5–4 yr) to the time of diagnosis, growth rates were reduced for patients with hypothalamic tumor involvement. Patients without hypothalamic involvement presented reduced growth rates in early infancy (age 10–12 months) that persisted until diagnosis. We conclude that reduced growth rates occur quite early in history; BMI SDS increases occur later and are predictive of obesity. Hypothalamic involvement is the major risk factor for obesity in patients with childhood craniopharyngioma.

Molecular-Level Characterization of Refinery Streams by High-Resolution Mass Spectrometry

2015 ◽  
Vol 29 (5) ◽  
pp. 2940-2950 ◽  
Author(s):  
Vatsala Sugumaran ◽  
Hillol Biswas ◽  
Anil Yadav ◽  
Jayaraj Christopher ◽  
Vivekanand Kagdiyal ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Molecular Level ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
Resolution Mass

Characterization of polymer/epoxy buried interfaces with silane adhesion promoters before and after hygrothermal aging for the elucidation of molecular level details relevant to adhesion

RSC Advances ◽  
2015 ◽  
Vol 5 (128) ◽  
pp. 105622-105631 ◽  
Author(s):  
Nathan W. Ulrich ◽  
John N. Myers ◽  
Zhan Chen
Keyword(s):  
Interfacial Adhesion ◽  
Molecular Level ◽  
Adhesion Properties ◽  
Hygrothermal Aging ◽  
Adhesion Promoters ◽  
Buried Interfaces ◽  
Interfacial Structures ◽  
Before And After ◽  
Microelectronics Industry

Buried interfacial structures containing epoxy underfills are incredibly important in the microelectronics industry and their structures determine the interfacial adhesion properties and ultimately their lifetime.

Sign in / Sign up

Export Citation Format

Share Document