Effect of Inorganic Phosphate on the Levels of Amino Acids in Suspension-cultured Cells of Catharanthus roseus*

1987 ◽  
Vol 60 (1) ◽  
pp. 109-114 ◽  
Author(s):  
TOSHIKO UKAJI ◽  
HIROSHI ASHIHARA
Keyword(s):  
Amino Acids ◽  
Catharanthus Roseus ◽  
Inorganic Phosphate ◽  
Cultured Cells ◽  
Suspension Cultured Cells

The Short-Term Effects of Inorganic Phosphate on the Levels of Metabolites in Suspension-Cultured Catharanthus roseus Cells

1989 ◽  
Vol 44 (9-10) ◽  
pp. 802-806 ◽  
Author(s):  
Kaoru Kubota ◽  
Xiao-Ni Li ◽  
Hiroshi Ashihara
Keyword(s):  
Amino Acids ◽  
Energy Metabolism ◽  
Catharanthus Roseus ◽  
Inorganic Phosphate ◽  
Cultured Cells ◽  
Short Term ◽  
Skoog Medium ◽  
Initial Values ◽  
Murashige Skoog ◽  
Stimulation Of

Abstract In order to analyze the effects of inorganic phosphate (Pi) on the energy metabolism of suspen­sion-cultured cells of Catharanthus roseus, short-term changes in levels of glycolytic metabolites were monitored after the addition of 1.25 mм Pi to cultures of cells previously cultured in a Pi-free Murashige-Skoog medium for 24 h. The levels of all phosphorylated intermediates of glycolysis examined were found to increase after addition of Pi. The most striking increases were observed in levels of glucose-6-phosphate and fructose-6-phosphate which rose to approximately 8 (after 30 min) and 15 times (after 120 min) their initial values. The levels of ATP and pyrophosphate doubled during the first 30 min of incubation. In contrast, the level of pyruvate decreased significantly during the initial 30 min and then began to increase again for the next 90 min. The short­ term effects of Pi on the rates of synthesis of proteins and RNA were estimated from the rates of incorporation of 14C-labelled amino acids and uridine into the macromolecules. No appreciable stimulation of the synthesis of proteins and RNA was induced by Pi within 120 min after the addition of Pi. Possible mechanisms are discussed that may be involved in the changes in metabo­lism initiated by the addition of Pi to the cultures.

Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status

Planta ◽  
2006 ◽  
Vol 225 (3) ◽  
pp. 711-718 ◽  
Author(s):  
Miwa Ohnishi ◽  
Tetsuro Mimura ◽  
Tomoko Tsujimura ◽  
Naoto Mitsuhashi ◽  
Setsuko Washitani-Nemoto ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Inorganic Phosphate ◽  
Cultured Cells ◽  
Phosphate Uptake ◽  
Suspension Cultured Cells

Mevalonate kinase activity in Catharanthus roseus plants and suspension cultured cells

Plant Science ◽  
2000 ◽  
Vol 150 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Anna E Schulte ◽  
Eva M Llamas Durán ◽  
Robert van der Heijden ◽  
Robert Verpoorte
Keyword(s):  
Catharanthus Roseus ◽  
Kinase Activity ◽  
Cultured Cells ◽  
Mevalonate Kinase ◽  
Suspension Cultured Cells

A novel terpenoid indole alkaloid derived from catharanthine via biotransformation by suspension-cultured cells of Catharanthus roseus

2015 ◽  
Vol 37 (12) ◽  
pp. 2481-2487 ◽  
Author(s):  
Shuijie He ◽  
Jianhua Zhu ◽  
Jiachen Zi ◽  
Pengfei Zhou ◽  
Jincai Liang ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Cultured Cells ◽  
Indole Alkaloid ◽  
Terpenoid Indole Alkaloid ◽  
Suspension Cultured Cells
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