scholarly journals Engineering Plant Resistance to Tomato Yellow Leaf Curl Thailand Virus Using a Phloem-Specific Promoter Expressing Hairpin RNA

2020 ◽  
Vol 33 (1) ◽  
pp. 87-97
Author(s):  
Yuh Tzean ◽  
Ho-Hsiung Chang ◽  
Tsui-Chin Tu ◽  
Bo-Han Hou ◽  
Ho-Ming Chen ◽  
...  
Keyword(s):  
Chlorophyll Synthesis ◽  
Tomato Yellow Leaf ◽  
Cauliflower Mosaic Virus ◽  
Rice Tungro Bacilliform Virus ◽  
35S Promoter ◽  
Yellow Leaf ◽  
Hairpin Rna ◽  
Leaf Curl Disease ◽  
Inoculation Assay ◽  
Leaf Curl

Transgenic approaches employing RNA interference (RNAi) strategies have been successfully applied to generate desired traits in plants; however, variations between RNAi transgenic siblings and the ability to quickly apply RNAi resistance to diverse cultivars remain challenging. In this study, we assessed the promoter activity of a cauliflower mosaic virus 35S promoter (35S) and a phloem-specific promoter derived from rice tungro bacilliform virus (RTBV) and their efficacy to drive RNAi against the endogenous glutamate-1-semialdehyde aminotransferase gene (GSA) that acts as a RNAi marker, through chlorophyll synthesis inhibition, and against tomato yellow leaf curl Thailand virus (TYLCTHV), a begomovirus (family Geminiviridae) reported to be the prevalent cause of tomato yellow leaf curl disease (TYLCD) in Taiwan. Transgenic Nicotiana benthamiana expressing hairpin RNA of GSA driven by either the 35S or RTBV promoter revealed that RTBV::hpGSA induced stronger silencing along the vein and more uniformed silencing phenotype among its siblings than 35S::hpGSA. Analysis of transgenic N. benthamiana, 35S::hpTYLCTHV, and RTBV::hpTYLCTHV revealed that, although 35S::hpTYLCTHV generated a higher abundance of small RNA than RTBV::hpTYLCTHV, RTBV::hpTYLCTHV transgenic plants conferred better TYLCTHV resistance than 35S::hpTYLCTHV. Grafting of wild-type (WT) scions to TYLCTHV RNAi rootstocks allowed transferable TYLCTHV resistance to the scion. A TYLCTHV-inoculation assay showed that noninfected WT scions were only observed when grafted to RTBV::hpTYLCTHV rootstocks but not 35S::hpTYLCTHV nor WT rootstocks. Together, our findings demonstrate an approach that may be widely applied to efficiently confer TYLCD resistance.

scholarly journals A DNAβ Associated with Tomato Yellow Leaf Curl China Virus Is Required for Symptom Induction

2004 ◽  
Vol 78 (24) ◽  
pp. 13966-13974 ◽  
Author(s):  
Xiaofeng Cui ◽  
Xiaorong Tao ◽  
Yan Xie ◽  
Claude M. Fauquet ◽  
Xueping Zhou
Keyword(s):  
Transgenic Plants ◽  
Stop Codon ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Tomato Yellow Leaf ◽  
Cauliflower Mosaic Virus ◽  
Start Codon ◽  
35S Promoter ◽  
Yellow Leaf ◽  
Leaf Curl

ABSTRACT We report here that all 25 isolates of Tomato yellow leaf curl China virus (TYLCCNV) collected from tobacco, tomato, or Siegesbeckia orientalis plants in different regions of Yunnan Province, China, were associated with DNAβ molecules. To investigate the biological role of DNAβ, full-length infectious clones of viral DNA and DNAβ of TYLCCNV isolate Y10 (TYLCCNV-Y10) were agroinoculated into Nicotiana benthamiana, Nicotiana glutinosa, Nicotiana. tabacum Samsun (NN or nn), tomato, and petunia plants. We found that TYLCCNV-Y10 alone could systemically infect these plants, but no symptoms were induced. TYLCCNV-Y10 DNAβ was required, in addition to TYLCCNV-Y10, for induction of leaf curl disease in these hosts. Similar to TYLCCNV-Y10, DNAβ of TYLCCNV isolate Y64 was also found to be required for induction of typical leaf curl diseases in the hosts tested. When the βC1 gene of TYLCCNV-Y10 DNAβ was mutated, the mutants failed to induce leaf curl symptoms in N. benthamiana when coinoculated with TYLCCNV-Y10. However, Southern blot hybridization analyses showed that the mutated DNAβ molecules were replicated. When N. benthamiana and N. tabacum plants were transformed with a construct containing the βC1 gene under the control of the Cauliflower mosaic virus 35S promoter, many transgenic plants developed leaf curl symptoms similar to those caused by a virus, the severity of which paralleled the level of βC1 transcripts, while transgenic plants transformed with the βC1 gene containing a stop codon after the start codon remained symptomless. Thus, expression of a βC1 gene is adequate for induction of symptoms of viral infection in the absence of virus.

scholarly journals High Expression of Truncated Viral Rep Protein Confers Resistance to Tomato Yellow Leaf Curl Virus in Transgenic Tomato Plants

1997 ◽  
Vol 10 (5) ◽  
pp. 571-579 ◽  
Author(s):  
A. Brunetti ◽  
M. Tavazza ◽  
E. Noris ◽  
R. Tavazza ◽  
P. Caciagli ◽  
...  
Keyword(s):  
Tomato Yellow Leaf ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Wild Type Plant ◽  
Yellow Leaf ◽  
Wild Type ◽  
Rep Protein ◽  
Plant Line ◽  
Leaf Curl Virus ◽  
Leaf Curl

A truncated version of the C1 gene of tomato yellow leaf curl geminivirus (TYLCV), encoding the first 210 amino acids of the multifunctional Rep protein, was introduced by Agrobacterium transformation into Lycopersicon esculentum cv. Moneymaker plants under the transcriptional control of an enhanced cauliflower mosaic virus 35S promoter. One R0 plant (line 47) carrying the C1 gene in two loci (A and B) and accumulating the truncated Rep protein (T-Rep), was crossed with either a wild-type plant, or a C1 antisense plant (line 10). The wild type (wt) × 47 progeny were phenotypically homogeneous, contained either A or B locus, expressed high levels of T-Rep protein, had a “curled” phenotype, and were resistant to TYLCV when challenged either by agroinfection or by the vector Bemisia tabaci. In the 10 × 47 progeny, plants carrying only the sense gene behaved like the wt × 47 progeny, while those containing both sense and antisense transgenes did not accumulate the T-Rep protein, showed a normal phenotype, and were not resistant, showing that accumulation of T-Rep protein is required to confer TYLCV resistance. Plants accumulating T-Rep were susceptible to a distinct geminivirus, tomato leaf curl virus (ToLCV-Au).

Mapping of QTLs in tomato line FLA456 associated with resistance to a virus causing tomato yellow leaf curl disease

Euphytica ◽  
2012 ◽  
Vol 190 (2) ◽  
pp. 297-308 ◽  
Author(s):  
P. Kadirvel ◽  
R. de la Peña ◽  
R. Schafleitner ◽  
S. Huang ◽  
S. Geethanjali ◽  
...  
Keyword(s):  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Tomato Line ◽  
Leaf Curl Disease ◽  
Leaf Curl

Tomato Plant Flavonoids Increase Whitefly Resistance and Reduce Spread of Tomato yellow leaf curl virus

2019 ◽  
Author(s):  
Qixi Yao ◽  
Zhengke Peng ◽  
Hong Tong ◽  
Fengbo Yang ◽  
Gaoshan Xing ◽  
...  
Keyword(s):  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Electrical Penetration Graph ◽  
Severe Damage ◽  
The World ◽  
New Varieties ◽  
Leaf Curl Virus ◽  
Phloem Feeding ◽  
Leaf Curl Disease ◽  
Leaf Curl

Abstract Tomato yellow leaf curl virus (TYLCV), a begomovirus (genus Begomovirus) is the causal agent of tomato yellow leaf curl disease (TYLCD), which causes severe damage to tomato (Solanum lycopersicum) crops throughout tropical and subtropical regions of the world. TYLCV is transmitted by the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in a circulative and persistent manner. Our previous studies showed that tomato flavonoids deter B. tabaci oviposition, but the effects of tomato flavonoids on the settling and feeding behavior of B. tabaci and on its transmission of TYLCV are unknown. Using two near-isogenic tomato lines that differ greatly in flavonoid levels, we found that high flavonoid production in tomato deterred the landing and settling of B. tabaci. Moreover, electrical penetration graph studies indicated that high flavonoid levels in tomato reduced B. tabaci probing and phloem-feeding efficiency. As a consequence, high flavonoid levels in tomato reduced the primary and secondary spread of TYLCV. The results indicate that tomato flavonoids provide antixenosis resistance against B. tabaci and that the breeding of such resistance in new varieties could enhance TYLCD management.

Genetic structure and evolution of natural populations of viruses causing the tomato yellow leaf curl disease in Spain

2007 ◽  
Vol 128 (1-2) ◽  
pp. 43-51 ◽  
Author(s):  
María Isabel Font ◽  
Luis Rubio ◽  
Pedro Vicente Martínez-Culebras ◽  
Concepción Jordá
Keyword(s):  
Genetic Structure ◽  
Natural Populations ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Leaf Curl Disease ◽  
Leaf Curl
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