scholarly journals Late Season Occurrence of Soybean Rust Caused by Phakopsora pachyrhizi on Soybean in Illinois

Plant Disease ◽  
2007 ◽  
Vol 91 (4) ◽  
pp. 466-466 ◽  
Author(s):  
G. L. Hartman ◽  
R. A. Hines ◽  
C. D. Faulkner ◽  
T. N. Lynch ◽  
N. Pataky
Keyword(s):  
Plant Disease ◽  
The Other ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Morphological Examination ◽  
University Of Illinois ◽  
Physiological Maturity ◽  
Disease Clinic ◽  
Leaf Surfaces ◽  
The University

Soybean rust, first reported in the continental United States in Louisiana in 2004 (2), is one of the most important foliar diseases of soybean worldwide. On 10 October 2006, 20 soybean leaflets from 20 plants at physiological maturity were arbitrarily collected in research plots near Glendale, IL at the University of Illinois Dixon Springs Agricultural Center in Pope County and sent by overnight courier. On 11 October, leaflets were examined with a dissecting microscope at the Soybean Disease Laboratory at the National Soybean Research Center, and then at the Plant Disease Clinic, University of Illinois. Tan, angular lesions that were 2 to 4 mm in diameter were observed on the lower leaf surfaces of two of the 20 leaflets. Within these lesions, there was one uredinum on one leaflet and four on the other leaflet exuding hyaline, echinulate urediniospores (20 × 25 μm). On 11 October 2006, these leaflets were sent by overnight courier to the USDA/APHIS/PPQ/NIS Laboratory, Beltsville, MD Plant Disease Clinic for identification by morphological examination and by PCR using primers specific to Phakopsora pachyrhizi (1). Both tests confirmed the presence of P. pachyrhizi. The 18 leaflets that did not have sporulating pustules on 11 October were incubated in the laboratory for 5 days at near 100% relative humidity. Following incubation, nine leaflets were observed to have uredinia exuding urediniospores with a range of 1 to 43 uredinia per leaflet. These results indicate that incubation may be necessary to maximize the potential to observe uredinia exuding urediniospores. To our knowledge, this is the first report of P. pachyrhizi infecting plants in Illinois. References: (1) R. D. Frederick et al. Phytopathology 92:217, 2002. (2) R. W. Schneider et al. Plant Dis. 89:774, 2005.

scholarly journals PSIII-24 True nutrient and amino acid digestibility of commercial pork-based extruded dog foods using the precision-fed cecectomized rooster assay

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 275-275
Author(s):  
Patricia M Oba ◽  
Kelly S Swanson
Keyword(s):  
Amino Acid ◽  
Mixed Models ◽  
Nutrient Digestibility ◽  
The Other ◽  
Protein Source ◽  
University Of Illinois ◽  
Feed Withdrawal ◽  
The University ◽  
Animal Care ◽  
Mixed Protein

Abstract Pork-based dog foods are increasing in popularity, but there has been a lack of research conducted on these diets, including information about their digestibility. Therefore, the objective of this experiment was to determine the true nutrient and amino acid (AA) digestibilities of commercial pork-based extruded dog foods using the precision-fed cecectomized rooster assay. Four commercial extruded diets were tested in this study, including three pork formulas (PF1; PF2; PF3) with varying levels of legume content, and a multi-protein formula (MPF), all provided by Champion Petfoods (Alberta, Canada). A precision-fed rooster assay utilizing cecectomized roosters was conducted to determine the true nutrient digestibility and standardized AA digestibilities of the diets tested. All animal procedures were approved by the University of Illinois Institutional Animal Care and Use Committee prior to experimentation. 16 cecectomized roosters (4 roosters/substrate) were randomly assigned to test substrates. After 24h of feed withdrawal, roosters were tube-fed 30g of test substrates. Following crop intubation, excreta (urine and feces) were collected for 48h. Endogenous corrections for AA were made using 5 additional cecectomized roosters. All data were analyzed using the Mixed Models procedure of SAS 9.4. There were no significant differences in true macronutrient digestibilities among diets tested. For most of the indispensable AA, digestibilities were greater than 80%, with some being greater than 90%. For the majority of indispensable and dispensable AA, MPF had higher (P < 0.05) AA digestibilities than the other diets tested. For the majority of indispensable AA, PF1 had the lowest AA digestibilities. In general, the diet containing a mixed protein source had the greatest AA digestibilities, but all diets including those based on pork protein performed well.

scholarly journals Comparisons of Visual Rust Assessments and DNA Levels of Phakopsora pachyrhizi in Soybean Genotypes Varying in Rust Resistance

Plant Disease ◽  
2011 ◽  
Vol 95 (8) ◽  
pp. 1007-1012 ◽  
Author(s):  
C. Paul ◽  
C. B. Hill ◽  
G. L. Hartman
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Single Gene ◽  
The Other ◽  
Soybean Rust ◽  
Disease Assessment ◽  
Fungal Colonization ◽  
Phakopsora Pachyrhizi ◽  
Breeding Populations ◽  
Soybean Genotypes ◽  
Infection Types

Soybean resistance to Phakopsora pachyrhizi, the cause of soybean rust, has been characterized by the following three infection types: (i) immune response (IM; complete resistance) with no visible lesions, (ii) resistant reaction with reddish brown (RB) lesions (incomplete resistance), and (iii) susceptible reaction with tan-colored (TAN) lesions. Based on visual assessments of these phenotypes, single gene resistance in soybean to P. pachyrhizi has been documented, but colonization within infected tissues based on fungal DNA (FDNA) levels in different soybean genotypes had not been analyzed. The research used a quantitative polymerase chain reaction (Q-PCR) assay to compare visual disease assessment to FDNA in controlled inoculation experiments using two isolates of P. pachyrhizi. The objective of the first experiment was to compare data from digital visual disease assessment to FDNA from Q-PCR assays using digital visual disease assessment using five resistant soybean genotypes (one IM and four RB) and five susceptible genotypes (TAN). The objective of the second experiment was to quantify FDNA using Q-PCR at different time points after inoculation to determine if levels of fungal colonization differed in five soybean genotypes with different levels of resistance (one IM, two RB, and two TAN). For experiment 1, the numbers of uredinia and uredinia per lesion on four of the five resistant soybean genotypes were lower (P < 0.05) than the other six genotypes. Significant differences (P < 0.05) in FDNA concentrations were found among soybean genotypes with TAN lesions and among soybean genotypes with RB lesions. Soybean cultivar UG5 (IM phenotype) had significantly less (P < 0.05) FDNA than all of the other genotypes. Some genotypes that produced TAN lesions had significantly lower (P < 0.05) or non-significantly different FDNA concentrations compared to those genotypes that produced RB lesions. For experiment 2, the regression of FDNA on days after inoculation was significant (P < 0.01) with positive slopes for all genotypes except for UG5, in which FDNA declined over time, indicating a reduction of fungal colonization. The results of this Q-PCR FDNA screening technique demonstrates its use to distinguish different types of resistance, and could be used to facilitate the evaluation of soybean breeding populations, where precise quantification of incomplete and/or partial resistance is needed to identify and map quantitative trait loci.

scholarly journals Performance of prothioconazole solo or added to mancozeb in the control of Asian soybean rust

2020 ◽  
Vol 46 (4) ◽  
pp. 345-347
Author(s):  
Erlei Melo Reis ◽  
Mateus Zanatta ◽  
Andrea Camargo Reis
Keyword(s):  
Field Experiment ◽  
Grain Yield ◽  
Factorial Design ◽  
The Other ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Site Specific ◽  
Asian Soybean Rust ◽  
Rust Severity

ABSTRACT The evolution of the reduction in Asian soybean rust (caused by Phakopsora pachyrhizi) control by site-specific fungicides has been reported season after season. In a field experiment, the effect of prothioconazole solo and added to multisite mancozeb was evaluated for rust control. Treatments were evaluated in a factorial design of four prothioconazole doses and three mancozeb doses. In a set of treatments, three applications were performed in one soybean cycle and four applications in another one. The first applications were performed at GS V8, 11 days before rust detection, with 2.56% leaflet incidence, while the other applications were at 12 to 14-day interval. Rust severity was quantified, control was calculated in relation to the unsprayed treatment, and soybean grain yield was estimated as kg/ha. Fifty-one to 61% control was obtained with three sprayings and 68% to 70% control with four sprayings of prothioconazole alone. Over 80% control was obtained with at least 0.3 L/ha prothioconazole + 2.0 kg/ha mancozeb, corresponding to 75 g a.i./ha prothioconazole + 1500 g a.i./ha mancozeb. Reduction in P. pachyrhizi control by the use of the site-specific fungicide alone was confirmed, while the addition of mancozeb can recover the efficacy of the site-specific fungicide.

scholarly journals Effects of Simplicillium lanosoniveum on Phakopsora pachyrhizi, the Soybean Rust Pathogen, and Its Use as a Biological Control Agent

2012 ◽  
Vol 102 (8) ◽  
pp. 749-760 ◽  
Author(s):  
N. A. Ward ◽  
C. L. Robertson ◽  
A. K. Chanda ◽  
R. W. Schneider
Keyword(s):  
Biological Control Agent ◽  
Signs And Symptoms ◽  
Control Agent ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Latent Stage ◽  
Leaf Surfaces ◽  
Rust Pathogen ◽  
Soybean Leaf ◽  
Soybean Leaves

The fungus Simplicillium lanosoniveum was isolated from soybean leaves infected with Phakopsora pachyrhizi, the soybean rust pathogen, in Louisiana and Florida. The fungus did not grow or become established on leaf surfaces until uredinia erupted, but when soybean rust signs and symptoms were evident, S. lanosoniveum colonized leaves within 3 days and sporulated within 4 days. Development of new uredinia was suppressed by about fourfold when S. lanosoniveum colonized uredinia. In the presence of S. lanosoniveum, uredinia became increasingly red-brown, and urediniospores turned brown and germinated at very low rates. Assays using quantitative real time polymerase chain reaction revealed that the fungus colonized leaf surfaces when plants were infected with P. pachyrhizi, either in a latent stage of infection or when symptoms were present. However, when plants were inoculated before infection, there was no increase of DNA of S. lanosoniveum, suggesting that the pathogen must be present in order for the antagonist to become established on soybean leaf surfaces. We documented significantly lower amounts of DNA of P. pachyrhizi and lower disease severity when soybean leaves were colonized with S. lanosoniveum. These studies documented the mycophilic and disease-suppressive nature of S. lanosoniveum.

Notes on the Language of Vita Aesopi G

Antichthon ◽  
1983 ◽  
Vol 17 ◽  
pp. 96-106
Author(s):  
† G.P. Shipp
Keyword(s):  
Tenth Century ◽  
First Century ◽  
The Other ◽  
Full Account ◽  
University Of Illinois ◽  
Strong Interest ◽  
The One ◽  
The University ◽  
Life Of Aesop

This version of the life of Aesop is known only from the one manuscript, which had belonged earlier to the library of a monastery near Frascati, from which it disappeared with no mention of it after 1789 till it was rediscovered in the Pierpont Morgan collection in 1929. It was published in 1952 by B.E. Perry at the University of Illinois Press (Urbana) in his fine Aesopica I, 35-77, with much other material, including a full account of the manuscripts of the other version of the life, W.MS. G is from the end of the tenth century. Perry thinks that the original goes back to the first century A.D. and reflects the strong interest in popular versions of Aesop’s life in Egypt at this period. It has a pronounced Egyptian colouring, Isis playing a prominent part in the naive and bawdy story, with a strong opposition to Apollo. Four papyrus fragments similar to G have been found (see Perry, op. cit. 1), and various Eastern versions of part of the story are known. The manuscript has many koine features that agree with Perry’s dating, and the language can often be usefully illustrated from the modern Demotic. Features that are more likely to be errors of tradition in the manuscript are mainly unimportant late spellings.

scholarly journals First Report of Soybean Rust Caused by Phakopsora pachyrhizi on Kudzu (Pueraria montana var. lobata) in Illinois

Plant Disease ◽  
2010 ◽  
Vol 94 (4) ◽  
pp. 477-477 ◽  
Author(s):  
C. A. Bradley ◽  
R. A. Hines ◽  
N. R. Pataky ◽  
J. S. Haudenshield ◽  
G. L. Hartman
Keyword(s):  
United States ◽  
The State ◽  
The Other ◽  
Soybean Rust ◽  
Southern United States ◽  
Phakopsora Pachyrhizi ◽  
Southern Illinois ◽  
Glycine Max L ◽  
Health Progress ◽  
Pueraria Montana

Soybean rust, caused by Phakopsora pachyrhizi Syd., first was observed in the continental United States during 2004 on soybean (Glycine max (L.) Merr.) in Louisiana (4), and on kudzu (Pueraria montana (Lour.) Merr. var. lobata (Willd.) Maesen & Almeida) in Florida (2). Kudzu is a leguminous weed that is prevalent in the southern United States with its range extending northward into other states including Illinois. In October 2009, a kudzu patch located in Pulaski County in southern Illinois was investigated for the presence of soybean rust. Twenty-five leaflets were collected, and the abaxial sides of leaflets were evaluated visually for the presence of uredinia with a dissecting microscope. Uredinia and urediniospores were found on two leaflets. When viewed with a compound microscope, urediniospores were hyaline, echinulate, and measured 20 × 25 μm. On the basis of uredinia and urediniospores, the disease tentatively was identified as soybean rust caused by P. pachyrhizi. To confirm the identification, one leaflet with pustules was assayed with a Soybean Rust QuickStix Diagnostic Kit (Envirologix, Portland, ME). For the other leaflet, the area of the pustule was excised (approximately 28 mm2) and an area of the leaflet at the margin on the opposite half of the leaflet with no visible pustule (approximately 54 mm2) was excised. DNA was extracted from the excised areas of the leaflet for confirmation by quantitative PCR (Q-PCR) using primers and probe specific to P. pachyrhizi and P. meibomiae (Arthur) Arthur (1). Both the QuickStix Diagnostic Kit and the Q-PCR confirmed the diagnosis as soybean rust caused by P. pachyrhizi. Q-PCR also suggested the presence of a nonsporulating latent rust infection on the same kudzu leaflet at the margin on the opposite side of the midrib. Soybean rust first was confirmed on soybean in Illinois in 2006 (3), but to our knowledge, this is the first observation of the disease on kudzu in the state. This report confirms that at least some kudzu plants in Illinois are susceptible to soybean rust and that latent kudzu infection may exist without outward signs of the fungus. Currently, this is the most northern observation of soybean rust on kudzu in North America. It is unknown what role, if any, Illinois kudzu will play in the epidemiology of soybean rust in the state. Since kudzu tops die after the first frost, there is no expectation of P. pachyrhizi to overwinter in Illinois on kudzu as it does in some states adjacent to the Gulf of Mexico. References: (1) R. D. Frederick et al. Phytopathology 92:217, 2002. (2) P. F. Harmon et al. Online publication. doi:10.1094/PHP-2005-0613-01-RS. Plant Health Progress, 2005. (3) G. L. Hartman et al. Plant Dis. 91:466, 2007. (4) R. W. Schneider et al. Plant Dis. 89:774, 2005.

scholarly journals Characterizing Resistance to Phakopsora pachyrhizi in Soybean

Plant Disease ◽  
2011 ◽  
Vol 95 (5) ◽  
pp. 577-581 ◽  
Author(s):  
M. R. Miles ◽  
M. R. Bonde ◽  
S. E. Nester ◽  
D. K. Berner ◽  
R. D. Frederick ◽  
...  
Keyword(s):  
Immune Response ◽  
Partial Resistance ◽  
Single Gene ◽  
Plant Introduction ◽  
The Other ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Progress Curve ◽  
Lesion Type ◽  
Incomplete Resistance

Resistance in soybean to Phakopsora pachyrhizi, the cause of soybean rust, is characterized by either reddish-brown (RB) lesions or an immune response. The RB type of resistance can be incomplete, as evidenced by the presence of sporulating uredinia within lesions. Susceptibility, on the other hand, is exemplified by tan-colored (TAN) lesions, and can be expressed in gradations of susceptibility or partial resistance that are less well defined. This study evaluated traits associated with incomplete or partial resistance to P. pachyrhizi in soybean by comparing 34 soybean accessions inoculated with four P. pachyrhizi isolates. Six accessions produced RB lesions to all four isolates, while 19 accessions produced TAN lesions, including plant introduction (PI) 200492 (Rpp1) and the susceptible check ‘Williams’. Williams had among the largest area under the disease progress curve (AUDPC) values and area under the sporulating uredinia progress curve (AUSUPC) values, while eight accessions had lower AUSUPC values. Of the known sources of single-gene resistance, only PI 230970 (Rpp2), PI 459025B (Rpp4), and PI 594538A (Rpp1b) had lower AUDPC and AUSUPC values than Williams. PI 594538A and PI 561356 had RB lesions and had the lowest AUDPC and AUSUPC values. Of the known sources of single-gene resistance, only PI 230970 (Rpp2) and PI 594538A (Rpp1b) produced fewer and smaller-diameter uredinia than Williams. This study characterized reactions to P. pachyrhizi in 34 accessions based on lesion type and sporulation, and defined incomplete resistance and partial resistance in the soybean–P. pachyrhizi interaction.

Media and Affect: A Comparison of Videotape, Audiotape, and Print

1980 ◽  
Vol 10 (3) ◽  
pp. 229-247 ◽  
Author(s):  
Ruth Stroud Machula
Keyword(s):  
Affective Response ◽  
Cognitive Learning ◽  
Semantic Differential ◽  
Affective Responses ◽  
The Other ◽  
Analysis Of Covariance ◽  
University Of Michigan ◽  
University Of Illinois ◽  
Video Group ◽  
The University

Students at the University of Illinois and the University of Michigan participated in an experiment to determine if different affective responses would result from exposure to three different forms of media, each presenting the same content. One group of students viewed a videotape, another listened to an audiotape, and a third read a printed transcript. A semantic differential was used to measure affective response, and an objective test was administered to measure cognitive learning. Results showed the video group to be perceiving the presentation less favorably than were the other two groups; however, they were perceiving two of the participants more favorably than were the others. An analysis of covariance between pre- and posttest scores of cognitive learning showed that subjects receiving the audiotape version had learned significantly less than those receiving the other treatments.

The Role of Visual Attention in Head-Up Displays: Design Implications for Varying Symbology Intensity

1995 ◽  
Vol 39 (1) ◽  
pp. 50-54 ◽  
Author(s):  
Patricia A. May ◽  
Christopher D. Wickens
Keyword(s):  
Visual Attention ◽  
Event Detection ◽  
Tracking Error ◽  
Weather Conditions ◽  
The Other ◽  
Focused Attention ◽  
University Of Illinois ◽  
The University ◽  
Cruise Flight

Twenty pilots from the University of Illinois flew a low fidelity simulator during cruise flight. The intensity of the display symbology was manipulated in three different weather conditions to influence the discriminability of the instrumentation. The symbology was displayed in either head-up or head-down locations, with equivalent optical distances and display formats. Half of the subjects flew with a conformal symbology set, while the other half flew with a partially conformal symbology set. Responses to near and far domain events were measured, and tracking error of the three axes of control was calculated. The results revealed a head-up advantage to the far domain event detection and a head-down advantage to the near domain event detection. Performance in the head-up condition approached that of the otherwise superior head-down condition when an appreciable contrast between the symbology and the background environment was provided. The results are discussed in terms of an effect of the modulation of focused attention.

scholarly journals First Report of Soybean Rust Caused by Phakopsora pachyrhizi on Kudzu (Pueraria montana var. lobata) in Kentucky

Plant Disease ◽  
2006 ◽  
Vol 90 (6) ◽  
pp. 834-834 ◽  
Author(s):  
D. E. Hershman ◽  
P. R. Bachi ◽  
C. L. Harmon ◽  
P. F. Harmon ◽  
M. E. Palm ◽  
...  
Keyword(s):  
United States ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Morphological Examination ◽  
Causal Organism ◽  
First Report ◽  
Monitoring Effort ◽  
Health Progress ◽  
On Line ◽  
Pueraria Montana

Phakopsora pachyrhizi, the causal organism of soybean rust, was first observed in the continental United States on 6 November 2004 (2). On 11 November 2005, as part a national soybean rust monitoring effort, 75 leaves of kudzu (Pueraria montana var. lobata) were arbitrarily collected from a patch growing in Princeton, Caldwell County, Kentucky (37.106650°N, 87.886120°W) that had been periodically scouted for the presence of the disease since May 2005. Upon microscopic examination of the nonincubated sample, a small (˜2.0 cm2) area of one leaf exhibited lesions, uredinia, and urediniospores characteristic of those reported for P. pachyrhizi (the Asian species) and P. meibomiae (the New World species) (2). No other infected leaves were observed despite repeated visits to the site and collection and observation of nearly 200 leaves. On 16 November 2005, one-half of the symptomatic tissue was sent by overnight courier to the USDA/APHIS/PPQ/NIS Laboratory, Beltsville, MD and the other half was sent to the Southern Plant Diagnostic Network Laboratory (SPDN), University of Florida, Gainesville. Both laboratories confirmed that the rust was a Phakopsora spp. on the basis of morphological examination. The preliminary polymerase chain reaction (PCR) testing conducted by the SPDN according to Harmon et al. (1) indicated the presence of P. pachyrhizi that was confirmed by the USDA/NPGBL using the validated modified real-time PCR assay described previously (2). The field diagnosis of P. pachyrhizi and preliminary PCR results were officially confirmed by USDA/APHIS on 18 November 2005. To our knowledge, this is the first report of P. pachyrhizi on kudzu or any host in Kentucky, and currently, the northernmost report of soybean rust on any host in the continental United States. References: (1) P. F. Harmon et al. On-line publication, doi:10.1094/PHP-2005-0613-O1-RS. Plant Health Progress, 2005. (2) R. W. Schneider et al. Plant Dis. 89:774, 2005.

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