Use of Detached Leaves to Evaluate Tobacco Haploids and Doubled Haploids for Resistance to Tobacco Mosaic Virus,Meloidogyne incognita,andPseudomonas syringaepv.tabaci

1987 ◽  
Vol 77 (1) ◽  
pp. 60 ◽  
Author(s):  
Rebeca C. Rufty
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Meloidogyne Incognita ◽  
Doubled Haploids ◽  
Detached Leaves

Ethylene production by detached leaves infected with tobacco mosaic virus

Virology ◽  
1970 ◽  
Vol 40 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Y. Nakagaki ◽  
T. Hirai ◽  
Mark A. Stahmann
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ethylene Production ◽  
Detached Leaves

scholarly journals Screening Tomato Seedlings for Multiple Disease Resistance

1992 ◽  
Vol 117 (4) ◽  
pp. 622-627 ◽  
Author(s):  
W. Alan Erb ◽  
Randall C. Rowe
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Meloidogyne Incognita ◽  
Root Rot ◽  
The Other ◽  
Spore Suspension ◽  
Tomato Seedlings ◽  
Multiple Disease Resistance ◽  
Crown And Root Rot ◽  
Time Required

Two procedures for screening tomato (Lycopersicon esculentum Mill.) seedlings for resistance to three pathogens were developed. In one scheme, seeds were sprayed with a spore suspension of Fusarium oxysporum f. sp. radicis-lycopersici Jarvis & Shoemaker (fusarium crown and root rot). Resistant seedlings were root-dipped 2.5 weeks later in a spore suspension of Verticillium dahliae Kleb. (verticillium wilt), and 1 week following the root dip, leaves were rubbed with tobacco mosaic virus. In the other scheme, 2-week-old seedlings were dipped in a spore suspension of F. oxysporum Schlecht f. sp. lycopersici (Sacc.) Snyd. & Hans. races 1 and 2 (fusarium wilt). Resistant seedlings were root-drenched 1.5 weeks later with a suspension of Meloidogyne incognita Kofoid & White (rootknot nematode), and 1 week following, the leaves were rubbed with tobacco mosaic virus. These procedures were effective for disease screening, and their use should reduce the time required for development of two multiple disease-resistant populations. Inbreds from each population could be crossed to produce hybrids resistant to five pathogens.

Quantitative Measurements on the Ion Etching of TMV

1973 ◽  
Vol 31 ◽  
pp. 336-337
Author(s):  
Irwin Bendet ◽  
Nabil Rizk
Keyword(s):  
Electron Microscope ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ion Current ◽  
Beam Diameter ◽  
Ion Etching ◽  
Preliminary Results ◽  
Quantitative Measurements ◽  
Monitoring Devices

Preliminary results reported last year on the ion etching of tobacco mosaic virus indicated that the diameter of the virus decreased more rapidly at 10KV than at 5KV, perhaps reaching a constant value before disappearing completely.In order to follow the effects of ion etching on TMV more quantitatively we have designed and built a second apparatus (Fig. 1), which incorporates monitoring devices for measuring ion current and vacuum as well as accelerating voltage. In addition, the beam diameter has been increased to approximately 1 cm., so that ten electron microscope grids can be exposed to the beam simultaneously.

Tobacco Mosaic Virus-Infected Tissue

1985 ◽  
Vol 43 ◽  
pp. 510-511
Author(s):  
Egbert W. Henry
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Chlorogenic Acid ◽  
Mosaic Virus ◽  
Host Resistance ◽  
Oxidative Metabolism ◽  
Leaf Tissue ◽  
Vein Clearing ◽  
Basal Septum ◽  
Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

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