scholarly journals Diabetes‐related alterations in second messenger regulation of potassium channel activity in corporal smooth muscle cells

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Weixin Zhao ◽  
Peter R. Brink ◽  
George J Christ
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Potassium Channel ◽  
Second Messenger ◽  
Muscle Cells ◽  
Channel Activity ◽  
Potassium Channel Activity

Fatty acid modifies Ca 2+ -dependent potassium channel activity in smooth muscle cells from the human aorta

1989 ◽  
Vol 237 (1288) ◽  
pp. 259-266 ◽  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Single Channel ◽  
Membrane Surface ◽  
Muscle Cells ◽  
Human Aorta ◽  
Channel Activity ◽  
Patch Clamp Technique ◽  
Membrane Fluidization ◽  
Potassium Channel Activity

By using the patch–clamp technique the effect of 2-decenoic acid (DA) on Ca 2+ -activated potassium (K + ) channels in the membrane of smooth muscle cells from the human aorta was studied. In the presence of 0.5 μM Ca 2+ and 2 mM Mg 2+ on the cytoplasmic side of the membrane, a more than tenfold elevation in the probability of the channels being open ( p o ) was observed under the effect of DA. With divalent cation concentrations of less than 1 nM DA caused a more than twofold elevation in p o . In the DA-treated membranes Mg 2+ ions, which normally fail to activate the channels, brought about a nearly threefold increase in the channel activity when applied to the inner membrane surface. Channel sensitivity to the activating effect of cytoplasmic Ca 2+ ions did not increase with the application of DA. Single-channel conductance was unchanged by DA exposure. We suggest that DA alters the Ca 2+ -binding mechanism of the channel, increasing its sensitivity to Mg 2+ ions, presumably owing to membrane fluidization.

Ketamine blocks Ca2+-activated K+ channels in rabbit cerebral arterial smooth muscle cells

2003 ◽  
Vol 285 (3) ◽  
pp. H1347-H1355 ◽  
Author(s):  
Jin Han ◽  
Nari Kim ◽  
Hyun Joo ◽  
Euiyong Kim
Keyword(s):  
Smooth Muscle ◽  
Patch Clamp ◽  
Smooth Muscle Cells ◽  
Cerebral Arteries ◽  
Muscle Cells ◽  
Channel Activity ◽  
Patch Clamp Technique ◽  
Arterial Smooth Muscle ◽  
Clamp Technique ◽  
Arterial Smooth Muscle Cells

Although ketamine and Ca2+-activated K+ (KCa) channels have been implicated in the contractile activity regulation of cerebral arteries, no studies have addressed the specific interactions between ketamine and the KCa channels in cerebral arteries. The purpose of this study was to examine the direct effects of ketamine on KCa channel activities using the patch-clamp technique in single-cell preparations of rabbit middle cerebral arterial smooth muscle. We tested the hypothesis that ketamine modulates the KCa channel activity of the cerebral arterial smooth muscle cells of the rabbit. Vascular myocytes were isolated from rabbit middle cerebral arteries using enzymatic dissociation. Single KCa channel activities of smooth muscle cells from rabbit cerebral arteries were recorded using the patch-clamp technique. In the inside-out patches, ketamine in the micromolar range inhibited channel activity with a half-maximal inhibition of the ketamine conentration value of 83.8 ± 12.9 μM. The Hill coefficient was 1.2 ± 0.3. The slope conductance of the current-voltage relationship was 320.1 ± 2.0 pS between 0 and +60 mV in the presence of ketamine and symmetrical 145 mM K+. Ketamine had little effect on either the voltage-dependency or open- and closed-time histograms of KCa channel. The present study clearly demonstrates that ketamine inhibits KCa channel activities in rabbit middle cerebral arterial smooth muscle cells. This inhibition of KCa channels may represent a mechanism for ketamine-induced cerebral vasoconstriction.

Effect of Testosterone on Potassium Channel Opening in Human Corporal Smooth Muscle Cells

2008 ◽  
Vol 5 (4) ◽  
pp. 822-832 ◽  
Author(s):  
Deok Hyun Han ◽  
Mee Ree Chae ◽  
Jae Hun Jung ◽  
Insuk So ◽  
Jong Kwan Park ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Potassium Channel ◽  
Muscle Cells ◽  
Channel Opening

scholarly journals Effects of ketamine on nicorandil induced ATP-sensitive potassium channel activity in cell line derived from rat aortic smooth muscle

2010 ◽  
Vol 57 (3,4) ◽  
pp. 237-244 ◽  
Author(s):  
Takashi Kawano ◽  
Katsuya Tanaka ◽  
Yinhua ◽  
Satoru Eguchi ◽  
Hiroaki Kawano ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Potassium Channel ◽  
Cell Line ◽  
Channel Activity ◽  
Aortic Smooth Muscle ◽  
Potassium Channel Activity
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