scholarly journals Manipulation of lipid bioaccessibility influences postprandial vitamin E concentrations in healthy human subjects: implications for inflammation, oxidative stress and vascular responses.

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Sarah E Berry ◽  
Karen Lapsley ◽  
Elizabeth A Tydeman ◽  
Hannah B Lewis ◽  
Ravneet Phalora ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Human Subjects ◽  
Healthy Human ◽  
Vascular Responses ◽  
Healthy Human Subjects

scholarly journals Hemorrhage simulated by lower body negative pressure provokes an oxidative stress response in healthy young adults

2019 ◽  
Vol 244 (3) ◽  
pp. 272-278
Author(s):  
Flora S Park ◽  
Victoria L Kay ◽  
Justin D Sprick ◽  
Alexander J Rosenberg ◽  
Garen K Anderson ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Negative Pressure ◽  
Lower Body Negative Pressure ◽  
Human Subjects ◽  
Oxidative Stress Response ◽  
Lower Body ◽  
Healthy Human ◽  
Healthy Human Subjects ◽  
Simulated Hemorrhage

Hemorrhage is a leading cause of potentially preventable death in both civilian and military trauma settings. Lower body negative pressure (LBNP) is a validated, non-invasive, and reproducible approach to simulate hemorrhage by inducing central hypovolemia in healthy conscious humans. The oxidative stress response to simulated hemorrhage via LBNP has not been quantified. We hypothesized that systemic markers of oxidative stress would increase with application of maximal, pre-syncopal limited LBNP. Fifteen healthy human subjects (11 M/4 F; age 27 ± 1 y) were recruited for a single LBNP experiment to presyncope (chamber pressure was progressively reduced every 5-min in a stepwise manner). Heart rate was assessed via ECG, arterial pressure and stroke volume (SV) were measured continuously via finger photoplethysmography, muscle oxygen saturation (SmO2) was measured via near-infrared spectroscopy, and venous blood samples were collected at baseline and presyncope. Plasma samples were analyzed for F2-isoprostanes (F2-IsoP), a global marker of oxidative stress. The magnitude of central hypovolemia, indexed by the maximal decrease (%Δ) in SV, ranged from 27 to 74% (53.5 ± 3.9%; P < 0.001), and mean arterial pressure (MAP) decreased by 12.6 ± 2.6% ( P < 0.001 vs. pre-LBNP baseline). F2-IsoP increased by 28.5 ± 11.6% ( P = 0.05) from baseline (24 ± 2 pg/mL) to presyncope (29 ± 3 pg/mL). The increase in F2-IsoP was not associated with %Δ SV ( r = 0.21, P = 0.46), %Δ MAP ( r = 0.05, P = 0.86), %Δ SmO2 ( r = 0.05, P = 0.90), or the maximum level of LBNP attained ( r = 0.35, P = 0.20). Simulated hemorrhage induced by LBNP to presyncope elicited an increase in oxidative stress, but this response was not associated with the magnitude of central hypovolemia, hypotension, or the decrease in peripheral muscle tissue oxygen saturation. These findings have important implications for the study of hemorrhage using LBNP, and future investigations of interventions targeting oxidative stress. Impact statement We characterize the systemic oxidative stress response in young, healthy human subjects with exposure to simulated hemorrhage via application of lower body negative pressure (LBNP). Prior work has demonstrated that LBNP and actual blood loss evoke similar hemodynamic and immune responses (i.e. white blood cell count), but it is unknown whether LBNP elicits oxidative stress resembling that produced by blood loss. We show that LBNP induces a 29% increase in F2-isoprostanes, a systemic marker of oxidative stress. The findings of this investigation may have important implications for the study of hemorrhage using LBNP, including future assessments of targeted interventions that may reduce oxidative stress, such as novel fluid resuscitation approaches.

scholarly journals Oxidised fish oil does not influence established markers of oxidative stress in healthy human subjects: a randomised controlled trial

2011 ◽  
Vol 108 (2) ◽  
pp. 315-326 ◽  
Author(s):  
Inger Ottestad ◽  
Gjermund Vogt ◽  
Kjetil Retterstøl ◽  
Mari C. Myhrstad ◽  
John-Erik Haugen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Fish Oil ◽  
Human Subjects ◽  
Controlled Study ◽  
Healthy Human ◽  
Significant Difference ◽  
Healthy Human Subjects ◽  
Markers Of Oxidative Stress ◽  
Randomised Controlled

Intake of fish oil reduces the risk of CHD and CHD deaths. Marine n-3 fatty acids (FA) are susceptible to oxidation, but to our knowledge, the health effects of intake of oxidised fish oil have not previously been investigated in human subjects. The aim of the present study was to investigate markers of oxidative stress, lipid peroxidation and inflammation, and the level of plasma n-3 FA after intake of oxidised fish oil. In a double-blinded randomised controlled study, healthy subjects (aged 18–50 years, n 54) were assigned into one of three groups receiving capsules containing either 8 g/d of fish oil (1·6 g/d EPA+DHA; n 17), 8 g/d of oxidised fish oil (1·6 g/d EPA+DHA; n 18) or 8 g/d of high-oleic sunflower oil (n 19). Fasting blood and morning spot urine samples were collected at weeks 0, 3 and 7. No significant changes between the different groups were observed with regard to urinary 8-iso-PGF2α; plasma levels of 4-hydroxy-2-hexenal, 4-hydroxy-2-nonenal and α-tocopherol; serum high sensitive C-reactive protein; or activity of antioxidant enzymes in erythrocytes. A significant increase in plasma level of EPA+DHA was observed in both fish oil groups, but no significant difference was observed between the fish oil groups. No changes in a variety of in vivo markers of oxidative stress, lipid peroxidation or inflammation were observed after daily intake of oxidised fish oil for 3 or 7 weeks, indicating that intake of oxidised fish oil may not have unfavourable short-term effects in healthy human subjects.

The effect of hydrogen peroxide-induced oxidative stress on leukocytes depends on age and physical training in healthy human subjects carrying the same genotypes of antioxidant enzymes' gene polymorphisms

2010 ◽  
Vol 22 (6) ◽  
pp. 807-812 ◽  
Author(s):  
Ana Luisa Miranda-Vilela ◽  
Penha Cristina Z. Alves ◽  
Arthur K. Akimoto ◽  
Luiz Carlos S. Pereira ◽  
Maria de Nazaré Klautau-Guimarães ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Enzymes ◽  
Physical Training ◽  
Gene Polymorphisms ◽  
Human Subjects ◽  
Healthy Human ◽  
Healthy Human Subjects

Bioavailability of catechins from guaraná (Paullinia cupana) and its effect on antioxidant enzymes and other oxidative stress markers in healthy human subjects

2016 ◽  
Vol 7 (7) ◽  
pp. 2970-2978 ◽  
Author(s):  
Lina Yonekura ◽  
Carolina Aguiar Martins ◽  
Geni Rodrigues Sampaio ◽  
Marcela Piedade Monteiro ◽  
Luiz Antônio Machado César ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Subjects ◽  
Antioxidant Enzyme Activity ◽  
Oxidative Stress Markers ◽  
Antioxidant Action ◽  
Healthy Individuals ◽  
Healthy Human ◽  
Paullinia Cupana ◽  
Stress Markers ◽  
Healthy Human Subjects

Guaraná catechins are bioavailable and reduce oxidative stress in healthy individuals by direct antioxidant action and increase of antioxidant enzyme activity.

scholarly journals Ergometry Induces Systemic Oxidative Stress in Healthy Human Subjects

2010 ◽  
Vol 221 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Maja Antoncic-Svetina ◽  
Davor Sentija ◽  
Ana Cipak ◽  
Davor Milicic ◽  
Andreas Meinitzer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Subjects ◽  
Healthy Human ◽  
Systemic Oxidative Stress ◽  
Healthy Human Subjects

Chronic stress decreases circulating endocannabinoid 2-arachidonoylglycerol in healthy human subjects

2015 ◽  
Author(s):  
Buqing Yi ◽  
Igor Nichiporuk ◽  
Matthias Feuerecker ◽  
Gustav Schelling ◽  
Alexander Chouker
Keyword(s):  
Chronic Stress ◽  
Human Subjects ◽  
Healthy Human ◽  
Healthy Human Subjects
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