RELATIONSHIP OF GLEASON SCORE (GS), PROSTATE SPECIFIC ANITGEN (PSA), CLINICAL STAGE (CS), AND SEMINAL VESICLE INVOLVEMENT (SVI) TO PELVIC LYMPH NODE (PLN) METASTASES AS DETERMINED BY REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) FOR PSA AND PROSTATE SPECIFIC MEMBRANE ANTIGEN (PSMA) IN MEN WITH PROSTATE CANCER

1999 ◽  
pp. 241
Author(s):  
Anna C. Ferrari ◽  
Nelson N. Stone ◽  
William Wallace ◽  
John Mandeli ◽  
Pam Unger ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Clinical Stage ◽  
Prostate Specific Membrane Antigen ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Seminal Vesicle Involvement ◽  
Polymerase Chain ◽  
Relationship Of ◽  
Specific Membrane

Enhanced detection of hematogenous circulating prostatic cells in patients with prostate adenocarcinoma by using nested reverse transcription polymerase chain reaction assay based on prostate-specific membrane antigen

1995 ◽  
Vol 41 (12) ◽  
pp. 1698-1704 ◽  
Author(s):  
S Loric ◽  
F Dumas ◽  
P Eschwege ◽  
P Blanchet ◽  
G Benoit ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Prostate Adenocarcinoma ◽  
Prostate Specific Membrane Antigen ◽  
Rt Pcr ◽  
Hematogenous Spread ◽  
Chain Reaction ◽  
Prostate Cells ◽  
Polymerase Chain ◽  
Specific Membrane

Abstract We report the development of a new sensitive nested reverse transcription-polymerase chain reaction (RT-PCR) assay, using primers derived from the prostate-specific membrane antigen (PSM) cDNA sequence, to detect an hematogenous spread of prostate adenocarcinoma cells. In 60 patients with a biopsy-proven prostate cancer, PSM and PSA RT-PCR detected circulating prostate cells in 40 and 20 patients, respectively. In pT4 M+ and pT3 M+ disease patients, nested PSM primers detected cells in 28 of 33 patients (85%), whereas nested PSA primers detected cells in 17 of 33 (51%). In patients with organ-confined cancer spread (pT2a and pT2b patients) before radical prostatectomy, nested PSM RT-PCR detected circulating prostatic epithelial cells in 6 of 17 patients (35%), which suggests that an hematogenous spread of prostate cells may occur early in prostate cancer history. Altogether, these results suggest that the detection of PSM-expressing cells in blood may predict the development of cancer in patients without clinically apparent prostate cancer. Nevertheless, the potential application and the clinical significance of detection of hematogenous prostate cells through the use of nested PSM primers need an extensive longitudinal study.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of circulating neoplastic cells by reverse-transcriptase polymerase chain reaction in malignant melanoma: association with clinical stage and prognosis.

1996 ◽  
Vol 14 (7) ◽  
pp. 2091-2097 ◽  
Author(s):  
B Mellado ◽  
D Colomer ◽  
T Castel ◽  
M Muñoz ◽  
E Carballo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Malignant Melanoma ◽  
Prognostic Factor ◽  
Peripheral Blood ◽  
Clinical Stage ◽  
Melanoma Cells ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Circulating Neoplastic Cells

PURPOSE Circulating melanoma cells can be detected in peripheral blood by means of tyrosinase mRNA amplification by reverse-transcriptase polymerase chain reaction (RT-PCR). We conducted a prospective study to evaluate the clinical significance of the presence of circulating neoplastic cells in the blood of patients with malignant melanoma (MM). METHODS A sensitive RT-PCR assay was used to detect tyrosinase mRNA in the peripheral blood of patients with stages I to IV melanoma. Healthy subjects or patients with other malignancies were used as negative controls. RESULTS Ninety-one assessable patients were included in the study. There was a statistically significant association between RT-PCR positivity and clinical stage. Circulating melanoma cells were detected in 36% of patients with localized disease (stages I and II), in 45% of patients with regional nodal involvement (stage III), and in 94% of patients with metastatic disease (stage IV) (P < .001). In stage II-III patients who were RT-PCR-positive for mRNA tyrosinase in blood, the recurrence rate and disease-free survival were significantly worse than patients who were RT-PCR-negative. In multivariate analysis, RT-PCR was an independent prognostic factor for recurrence in patients with nonmetastatic disease (P = .002). CONCLUSION The detection of circulating melanoma cells in peripheral blood by RT-PCR correlated with the clinical stage of patients with melanoma and was an independent prognostic factor for recurrence. Further studies are warranted to better assess the significance of this test in the evaluation of prognosis, early detection of relapse, and in monitoring the effectiveness of systemic therapy.

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