Expression of CD133 and other putative stem cell markers in uveal melanoma

2011 ◽  
Vol 21 (5) ◽  
pp. 405-416 ◽  
Author(s):  
Michelle Thill ◽  
Marc Jean Berna ◽  
Rebecca Grierson ◽  
Inna Reinhart ◽  
Tobias Voelkel ◽  
...  
2014 ◽  
Vol 9 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Willam Sterlacci ◽  
Spasenija Savic ◽  
Michael Fiegl ◽  
Ellen Obermann ◽  
Alexandar Tzankov

2013 ◽  
Vol 91 ◽  
pp. 0-0
Author(s):  
Z STEIBER ◽  
G HALMOS ◽  
Z CSERHATI ◽  
A TRESZL

Cancers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 5990
Author(s):  
Aaron B. Beasley ◽  
Timothy W. Isaacs ◽  
Tersia Vermeulen ◽  
James Freeman ◽  
Jean-Louis DeSousa ◽  
...  

(1) Background: The stratification of uveal melanoma (UM) patients into prognostic groups is critical for patient management and for directing patients towards clinical trials. Current classification is based on clinicopathological and molecular features of the tumour. Analysis of circulating tumour cells (CTCs) has been proposed as a tool to avoid invasive biopsy of the primary tumour. However, the clinical utility of such liquid biopsy depends on the detection rate of CTCs. (2) Methods: The expression of melanoma, melanocyte, and stem cell markers was tested in a primary tissue microarray (TMA) and UM cell lines. Markers found to be highly expressed in primary UM were used to either immunomagnetically isolate or immunostain UM CTCs prior to treatment of the primary lesion. (3) Results: TMA and cell lines had heterogeneous expression of common melanoma, melanocyte, and stem cell markers. A multi-marker panel of immunomagnetic beads enabled isolation of CTCs in 37/43 (86%) patients with UM. Detection of three or more CTCs using the multi-marker panel, but not MCSP alone, was a significant predictor of shorter progression free (p = 0.040) and overall (p = 0.022) survival. (4) Conclusions: The multi-marker immunomagnetic isolation protocol enabled the detection of CTCs in most primary UM patients. Overall, our results suggest that a multi-marker approach could be a powerful tool for CTC separation for non-invasive prognostication of UM.


The Prostate ◽  
2010 ◽  
Vol 71 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Andrey V. Ugolkov ◽  
Laurie J. Eisengart ◽  
Chunyan Luan ◽  
Ximing J. Yang

Author(s):  
Joseph Wahler ◽  
Jae Young So ◽  
Larry C. Cheng ◽  
Hubert Maehr ◽  
Milan Uskokovic ◽  
...  

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